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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 34, No. 4, 2013, pp. 399-405
Bioline Code: zr13062
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 34, No. 4, 2013, pp. 399-405

 en Cloning and expression analysis of the NFκB inhibitor IκBα of ayu ( Plecoglossus altivelis check for this species in other resources )
ZHANG, Wen-Qing; GONG, Yi-Fu; ZHANG, Li; LI, Jun & LIU, Xiao-Dan

Abstract

The NFκB inhibitor (IκBα) is an integral part of NFκB/IκB signaling pathways, which plays roles in a variety of immune responses, such as bacterial infection resistance. By interacting with nuclear transcription factor NFκB, IκBα controls a variety of biological immune gene expressions. In this study, full-length cDNA (1341 bp) of the NFκB inhibitor IκBα (PaIκBα, GenBank Accession No. JN801027) of Plecoglossus altivelis check for this species in other resources was obtained by RACE and PCR, and included a 5' untranslated region (UTR) (64 bp), a 3' untranslated region (UTR) (341 bp) and an open reading frame (ORF) (936 bp) encoding a polypeptide of 311 amino acids. PaIκBα had high homology with other IκBαs, containing a conserved ankyrin repeat domain, which was required for interacting with NFκB, a PEST sequence in the C-terminus and a signal responsive domain in the N-terminus. The deduced amino acid sequence of PaIκBα shared 95% homology with Osmerus mordax check for this species in other resources , and 76%, 75%, 70%, and 68% homology with Salmo salar check for this species in other resources , Oncorhynchus mykiss check for this species in other resources , Nile tilapia check for this species in other resources , and Siniperca chuatsi check for this species in other resources , respectively. Phylogenetic analysis revealed that IκBα of ayu and Osmerus mordax, Salmo salar, Oncorhynchus mykiss, Nile tilapia, and Siniperca chuatsi were in the same phylogenetic tree. RT-PCR analysis showed that PaIκBα mRNA expression was highest in the liver, kidney, intestine, and gills, then followed by the spleen, brain and muscle, and was lowly expressed in the heart. Likewise, after Aeromonas hydrophila check for this species in other resources infection, the mRNA level of ayu PaIκBα in the liver was also up-regulated.

Keywords
Plecoglossus altivelis; IκBα; Clone; Aeromonas hydrophila; Gene expression

 
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