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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 34, No. 5, 2013, pp. 446-452
Bioline Code: zr13069
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 34, No. 5, 2013, pp. 446-452

 en Establishment of full-sib families of Branchiostoma japonicum check for this species in other resources and the relationship between early development patterns and larvae survival rates
LI, Wei-Ye & WANG, Yi-Quan

Abstract

One general requirement of individual laboratory animals is that they have known genetic backgrounds. However, ensuring such genetic similarity is difficult, and can be facilitated by breeding a full strain for experimentation. To this end, the authors bred 34 full-sib families of amphioxus larvae/embryos. Due to the high mortality of the embryos and larvae, only seven full-sib families of juvenile amphioxus Branchiostoma japonicum check for this species in other resources were obtained. Among them, the highest and lowest survival ratios were 32.4% and 1.67%, respectively, whereas the shortest metamorphosis and longest larva duration were 24 d and 42 d, respectively. These results demonstrate the feasibility of establishing full-sib families of amphioxus, and provide fundamental data needed for the future breeding of amphioxus strains.

Keywords
Amphioxus; Full-sib family; Propagation; Development; Survival ratio

 
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