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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 34, No. 5, 2013, pp. 509-518
Bioline Code: zr13081
Full paper language: English
Document type: Review Article
Document available free of charge

Zoological Research, Vol. 34, No. 5, 2013, pp. 509-518

 en Transcription activator-like effectors(TALEs)based genome engineering
ZHAO, Mei-Wei; DUAN, Cheng-Li & LIU, Jiang

Abstract

Systematic reverse-engineering of functional genome architecture requires precise modifications of gene sequences and transcription levels. The development and application of transcription activator-like effectors(TALEs) has created a wealth of genome engineering possibilities. TALEs are a class of naturally occurring DNA-binding proteins found in the plant pathogen Xanthomonas check for this species in other resources species. The DNA-binding domain of each TALE typically consists of tandem 34-amino acid repeat modules rearranged according to a simple cipher to target new DNA sequences. Customized TALEs can be used for a wide variety of genome engineering applications, including transcriptional modulation and genome editing. Such "genome engineering" has now been established in human cells and a number of model organisms, thus opening the door to better understanding gene function in model organisms, improving traits in crop plants and treating human genetic disorders.

Keywords
TALEs; Transcriptional modulation; Genome editing; Double-strand break; Non-homologous end joining; Homologous recombination

 
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