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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 34, No. 6, 2013, pp. 610-616
Bioline Code: zr13096
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 34, No. 6, 2013, pp. 610-616

 en Application of ND-FISH in amphibians
CHANG, Xiao-Ai; XIA, Yun & ZENG, Xiao-Mao

Abstract

The recently popularized non-denaturing fluorescence in situ hybridization (ND-FISH) is a new technique that is both quick and efficient, in part because denaturing of both of the probes and the chromosomes is unnecessary. Synthetic simple sequence repeats (SSRs) labeled with fluorescein are used as probes to detect SSR-enriched chromosome regions and provide markers to identify the chromosomes. To date this method has not been applied to amphibians, even though the polymorphism of the distribution of SSRs may help to advance genetic polymorphism research. This paper also improved the double-colour FISH method by simultaneously using probes labelled with fluorescein and probes labelled with DIG to get double-color signals. This study found 5 SSRs markers that may be useful in the polymorphism research, and that the amphibian chromosomes must be denatured in ND-FISH.

Keywords
ND-FISH; SSRs; Marker selection; Double-colour FISH

 
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