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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 35, No. 2, 2014, pp. 81-91
Bioline Code: zr14009
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 35, No. 2, 2014, pp. 81-91

 en Sequencing and phylogenetic analysis of the Pyrgilauda ruficollis check for this species in other resources (Aves, Passeridae) complete mitochondrial genome
MA, Yong-Gui; HUANG, Yuan & LEI, Fu-Min

Abstract

In this study, both long PCR and conserved primers walking sequencing methods were used to determine the complete sequence of the of Pyrgilauda ruficollis check for this species in other resources mitochondrial genome (KC836121). The results showed that the complete mitochondrial genome of P. ruficollis is 1 6909 bp in length with 55.0% A+T content, harboring the typical 37 genes. The mitogenome had the same gene order with that of Podoces hendersoni check for this species in other resources All protein coding genes started with ATG codon, except ND3 with GTG. For the stop codon usage, most genes terminate with codons TAA or TAG, but ND5 terminated with AGA, while ND1 and COI genes with AGG, and both the genes COIII and ND4 have an incomplete termination codon (T). The secondary structures of 22 tRNA genes were also predicted, showing that all tRNAs can form typical clover-leaf secondary structures, except for the tRNASer (AGN) which loses the DHU arm, while tRNAPhe harbor an extra nucleotide inserted in the TψC arm. The predicted secondary structures of 12S rRNA and 16S rRNA exhibit 47 helices in 4 domains and 60 helices in 6 domains respectively. The control region of P. ruficollis with the length of 1 305 bp was located between tRNAGlu and tRNAPhe, and typical domains of which could be found as other bird groups. Using the data from 13 mitochondrial protein-coding genes, results of a final phylogenetic analysis strongly supports the traditional view that P. ruficollis is closely related with Passeridae and Fringillidae.

Keywords
Pyrgilauda ruficollis; Complete mitochondrial genome; Phylogeny

 
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