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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 35, No. 3, 2014, pp. 186-195
Bioline Code: zr14022
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 35, No. 3, 2014, pp. 186-195

 en Replication potentials of HIV-1/HSIV in PBMCs from northern pig-tailed macaque (Macaca leonina check for this species in other resources )
LEI, Ai-Hua; ZHANG, Gao-Hong; TIAN, Ren-Rong; ZHU, Jia-Wu; ZHENG, Hong-Yi; PANG, Wei & ZHENG, Yong-Tang

Abstract

The northern pig-tailed macaque (Macaca leonina check for this species in other resources ) has been identified as an independent species of Old World monkey, and we previously found that PBMCs from M. leonina were susceptible to human immunodeficiency virus type 1 (HIV-1), which may be due to the absence of a TRIM5 protein restricting HIV-1 replication. Here we investigated the infection potentials of six laboratory adapted HIV-1 strains and three primary HIV-1 isolates in PBMCs from M. leonina. The results indicate that these strains are characterized by various but low replication levels, among which, HIV-1NL4-3 shows the highest replication ability. Based on the abundant evidence of species-specific interactions between restriction factors APOBEC3 and HIV/SIV-derived Vif protein, we subsequently examined the replication potentials of vif -substituted HIV-1 (HSIV) in M. leonina PBMCs. Notably, HSIV-vifmac and stHIV-1SV chimeras, two HIV-1NL4-3-derived viruses encoding the viral infectivity factor (Vif) protein from SIVmac239, replicated robustly in cells from M. leonina, which suggests that HSIV could effectively antagonize the antiviral activity of APOBEC3 proteins expressed in cells of M. leonina. Therefore, our data demonstrate that M. leonina has the potential to be developed into a promising animal model for human AIDS.

Keywords
HIV-1; HSIV; Replication; PBMC; Northern pig-tailed macaque (Macaca leonina)

 
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