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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 35, No. 4, 2014, pp. 300-306
Bioline Code: zr14035
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 35, No. 4, 2014, pp. 300-306

 en Molecular cloning and function analysis of insulin-like growth factor-binding protein 1a in blunt snout bream ( Megalobrama amblycephala check for this species in other resources )
TIAN, Yu-Mei; CHEN, Jie; TAO, Yang; JIANG, Xia-Yun & ZOU, Shu-Ming

Abstract

Insulin-like growth factor-binding protein 1 (IGFBP-1), a hypoxia-induced protein, is a member of the IGFBP family that regulates vertebrate growth and development. In this study, full-length IGFBP-1a cDNA was cloned from a hypoxia-sensitive Cyprinidae fish species, the blunt snout bream ( Megalobrama amblycephala check for this species in other resources ). IGFBP-1a was expressed in various organs of adult blunt snout bream, including strongly in the liver and weakly in the gonads. Under hypoxia, IGFBP-1a mRNA levels increased sharply in the skin, liver, kidney, spleen, intestine and heart tissues of juvenile blunt snout bream, but recovered to normal levels after 24-hour exposure to normal dissolved oxygen. In blunt snout bream embryos, IGFBP-1a mRNA was expressed at very low levels at both four and eight hours post-fertilization, and strongly at later stages. Embryonic growth and development rates decreased significantly in embryos injected with IGFBP-1a mRNA. The average body length of IGFBP-1a-overexpressed embryos was 82.4% of that of the control group, and somite numbers decreased to 85.2%. These findings suggest that hypoxia-induced IGFBP-1a may inhibit growth in this species under hypoxic conditions.

Keywords
Megalobrama amblycephala; IGFBP-1a; Hypoxia; Overexpression

 
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