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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 35, No. 5, 2014, pp. 380-388
Bioline Code: zr14047
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 35, No. 5, 2014, pp. 380-388

 en Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel ( Monopterus albus check for this species in other resources )
Xu, Qiao-Qing; Yang, Dai-Qin; Tuo, Rui; Wan, Jing; Chang, Ming-Xian & Nie, Pin

Abstract

The Asian swamp eel ( Monopterus albus check for this species in other resources ) is one of the most economically important freshwater fish in East Asia, but data on the immune genes of M. albus are scarce compared to other commercially important fish. A better understanding of the eel's immune responses may help in developing strategies for disease management, potentially improving yields and mitigating losses. In mammals, interferon regulatory factors (IRFs) play a vital role in both the innate and adaptive immune system; though among teleosts IRF4 and IRF10 have seldom been studied. In this study, we characterized IRF4 and IRF10 from M. albus (maIRF4 and maIRF10) and found that maIRF4 cDNA consists of 1 716 nucleotides encoding a 451 amino acid (aa) protein, while maIRF10 consists of 1 744 nucleotides including an open reading frame (ORF) of 1 236 nt encoding 411 aa. The maIRF10 gene was constitutively expressed at high levels in a variety of tissues, while maIRF4 showed a very limited expression pattern. Expression of maIRF4 and maIRF10 in head kidney, and spleen tissues was significantly up-regulated from 12 h to 48 h post-stimulation with polyinosinic: polycytidylic acid (poly I:C), lipopolysaccharide (LPS) and a common pathogenic bacteria Aeromonas hydrophila check for this species in other resources . These results suggest that IRF4 and IRF10 play roles in immune responses to both viral and bacterial infections in M. albus.

Keywords
Monopterus albus; IRF4; IRF10; Poly I:C; Up-regulation

 
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