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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 35, No. 6, 2014, pp. 492-499
Bioline Code: zr14059
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 35, No. 6, 2014, pp. 492-499

 en Isolation and identification of symbiotic bacteria from the skin, mouth, and rectum of wild and captive tree shrews
LI, Gui; LAI, Ren; DUAN, Gang; LYU, Long-Bao; ZHANG, Zhi-Ye; LIU, Huang & XIANG, Xun

Abstract

Endosymbionts influence many aspects of their hosts' health conditions, including physiology, development, immunity, metabolism, etc. Tree shrews ( Tupaia belangeri chinensis check for this species in other resources ) have attracted increasing attention in modeling human diseases and therapeutic responses due to their close relationship with primates. To clarify the situation of symbiotic bacteria from their body surface, oral cavity, and anus, 12 wild and 12 the third generation of captive tree shrews were examined. Based on morphological and cultural characteristics, physiological and biochemical tests, as well as the 16S rDNA full sequence analysis, 12 bacteria strains were isolated and identified from the wild tree shrews: body surface: Bacillus subtilis check for this species in other resources (detection rate 42%), Pseudomonas aeruginosa check for this species in other resources (25%), Staphlococcus aureus check for this species in other resources (33%), S. Epidermidis check for this species in other resources (75%), Micrococcus luteus check for this species in other resources (25%), Kurthia gibsonii check for this species in other resources (17%); oral cavity: Neisseria mucosa check for this species in other resources (58%), Streptococcus pneumonia check for this species in other resources (17%); anus: Enterococcus faecalis check for this species in other resources (17%), Lactococus lactis check for this species in other resources (33%), Escherichia coli check for this species in other resources (92%), Salmonella typhosa (17%); whereas, four were indentified from the third generation captive tree shrews: body surface: S. epidermidis (75%); oral cavity: N.mucosa (67%); anus: L. lactis (33%), E. coli (100%). These results indicate that S. epidermidis, N. mucosa, L. lactis and E. coli were major bacteria in tree shrews, whereas, S. aureus, M. luteus, K. gibsonii, E. faecalis and S. typhosa were species-specific flora. This study facilitates the future use of tree shrews as a standard experimental animal and improves our understanding of the relationship between endosymbionts and their hosts.

Keywords
Tree shrew; Microbial; Separation; Identification

 
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