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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 35, No. 6, 2014, pp. 528-536
Bioline Code: zr14062
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 35, No. 6, 2014, pp. 528-536

 en Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation
HE, Ya; WANG, Zheng-Huan & WANG, Xiao-Ming

Abstract

Cervus sichuanicus is a species of sika deer ( Cervus nippon check for this species in other resources Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalskii check for this species in other resources . Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.

Keywords
Sichuan sika deer; Microsatellite; Genetic diversity; Population structure

 
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