About Bioline  All Journals  Testimonials  Membership  News

Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 36, No. 1, 2015, pp. 54-58
Bioline Code: zr15007
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 36, No. 1, 2015, pp. 54-58

 en Effects of alcohol on H3K9 acetylation in mouse pre-implantation embryos
DING, Fang; CHEN, Li; LIU, Yong; WU, Feng-Rui; DING, Biao; LI, Wen-Yong & WANG, Rong


It is well known that excessive long-term alcohol consumption is harmful, especially in pregnant women. In the present study, the Kunming white mouse was used as an animal model and indirect immunofluorescence was performed to analyze the toxic effects of alcohol on early pre-implantation embryos. H3K9 acetylation immunofluorescence could not be detected in MII oocytes. H3K9 acetylation levels in the treatment group were higher than in the control group during the morula stage, and contrary to results during the blastocyst stage. Other stages showed no obvious differences for in vivo embryos. For in vitro embryos, almost no difference was found between the two experimental groups across all stages, and both groups showed increasing H3K9 acetylation levels (except at the 2-cell stage). This study shows that H3K9 acetylation levels in early pre-implantation embryos are notably impacted by excessive alcohol ingestion by females. These data are the first step in understanding the epigenetic mechanism of alcohol toxicity in early pre-implantation mouse embryos.

Alcohol intake; Pre-implantation embryos; H3K9 acetylation; Mice; Embryo development

© Copyright 2015 - Zoological Research
Alternative site location:

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil