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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 36, No. 3, 2015, pp. 133-141
Bioline Code: zr15016
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 36, No. 3, 2015, pp. 133-141

 en Molecular characterization of an IL-1β gene from the large yellow croaker ( Larimichthys crocea check for this species in other resources ) and its effect on fish defense against Vibrio alginolyticus check for this species in other resources infection
WU, Jun; SHI, Yu-Hong; ZHANG, Xue-Heng; LI, Chang-Hong; LI, Ming-Yun & CHEN, Jiong

Abstract

Interleukin 1β (IL-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the cDNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea check for this species in other resources . Phylogenetic analysis indicated that the IL-1β (LcIL-1β) gene was most closely related to that of European seabass ( Dicentrarchus labrax check for this species in other resources ), sharing 67.8% amino acid identity. In healthy large yellow croaker, LcIL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus check for this species in other resources infection, LcIL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant LcIL-1β (rLcIL-1β) improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, rLcIL-1β induced monocytes/macrophages (MO/MΦ) chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that LcIL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.

Keywords
Interleukin 1β; Large yellow croaker; Survival rate; Vibrio alginolyticus; Monocytes/ macrophages

 
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