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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 36, No. 3, 2015, pp. 174-177
Bioline Code: zr15020
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 36, No. 3, 2015, pp. 174-177

 en Purification and characterization of cholecystokinin from the skin of salamander Tylototriton verrucosus check for this species in other resources
JIANG, Wen-Bin; HAKIM, Ma; LUO, Lei; LI, Bo-Wen; YANG, Shi-Long; SONG, Yu-Zhu; LAI, Ren & LU, Qiu-Min

Abstract

As a group of intestinal hormones and neurotransmitters, cholecystokinins (CCKs) regulate and affect pancreatic enzyme secretion, gastrointestinal motility, pain hypersensitivity, digestion and satiety, and generally contain a DYMGWMDFG sequence at the C-terminus. Many CCKs have been reported in mammals. However, only a few have been reported in amphibians, such as Hyla nigrovittata, Xenopus laevis check for this species in other resources , and Rana catesbeiana check for this species in other resources , with none reported in urodele amphibians like newts and salamanders. Here, a CCK called CCK-TV was identified and characterized from the skin of the salamander Tylototriton verrucosus check for this species in other resources . This CCK contained an amino acid sequence of DYMGWMDF-NH2 as seen in other CCKs. A cDNA encoding the CCK precursor containing 129 amino acid residues was cloned from the cDNA library of T. verrucosus skin. The CCK-TV had the potential to induce the contraction of smooth muscle strips isolated from porcine gallbladder, eliciting contraction at a concentration of 5.0x10-11 mol/L and inducing maximal contraction at a concentration of 2.0x10-6 mol/L. The EC50 was 13.6 nmol/L. To the best of our knowledge, this is the first report to identify the presence of a CCK in an urodele amphibian.

Keywords
Cholecystokinin; Salamander; Skin; Amphibian

 
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