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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 36, No. 4, 2015, pp. 182
Bioline Code: zr15028
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 36, No. 4, 2015, pp. 182

 en Extraction and identification of membrane proteins from black widow spider eggs
FU, Si-Ling; LI, Jiang-Lin; CHEN, Jia; WANG, Qiu-Ting; LI, Jian-Jun & WANG, Xian-Chun

Abstract

The eggs of oviparous animals are storehouses of maternal proteins required for embryonic development. Identification and molecular characterization of such proteins will provide much insight into the regulation of embryonic development. We previously analyzed soluble proteins in the eggs of the black widow spider ( Latrodectus tredecimguttatus check for this species in other resources ), and report here on the extraction and mass spectrometric identification of the egg membrane proteins. Comparison of different lysis solutions indicated that the highest extraction of the membrane proteins was achieved with 3%-4% sodium laurate in 40 mmol/L Tris-HCl buffer containing 4% CHAPS and 2% DTT (pH 7.4). SDS-PAGE combined with nLC-MS/MS identified 39 proteins with membrane-localization annotation, including those with structural, catalytic, and regulatory activities. Nearly half of the identified membrane proteins were metabolic enzymes involved in various cellular processes, particularly energy metabolism and biosynthesis, suggesting that relevant metabolic processes were active during the embryonic development of the eggs. Several identified cell membrane proteins were involved in the special structure formation and function of the egg cell membranes. The present proteomic analysis of the egg membrane proteins provides new insight into the molecular mechanisms of spider embryonic development.

Keywords
Latrodectus tredecimguttatus; Egg; Membrane protein; Extraction; Identification

 
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