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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 36, No. 5, 2015, pp. 305-309
Bioline Code: zr15033
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 36, No. 5, 2015, pp. 305-309

 en Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole ( Cynoglossus semilaevis check for this species in other resources )
WANG, Tian-Zi; SUN, Ai; WANG, Na; CUI, Zhong-Kai; CHEN, Song-Lin & SHA, Zhen-Xia

Abstract

An astroglial cell line was established from the brain of half smooth tongue sole ( Cynoglossus semilaevis check for this species in other resources ) and was designated as CSAC. CSAC shows the morphological homogeneity of epithelial cells. The cell identity was tested by the presence of glial fibrillary acidic protein (GFAP), which was revealed by RT-PCR and immunofluorescence. The cell line was optimally maintained at 24 °C in minimum essential medium supplemented with HEPES, antibiotics, 20% fetal bovine serum, 2-Mercaptoethanol (2-Me) and basic fibroblast growth factor. Chromosome analysis revealed that the CSAC cells maintained a normal diploid chromosome number (2n=42). The fluorescent signals were observed in CSAC after the cells were transfected with green fluorescent protein (GFP) reporter plasmids. The CSAC cell line may serve as a valuable tool for studies on the potential functions of fish astroglial cells.

Keywords
Half-smooth tongue sole; Brain astroglial cell; Cynoglossus semilaevis

 
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