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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 37, No. 2, 2016, pp. 96-102
Bioline Code: zr16016
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 37, No. 2, 2016, pp. 96-102

 en Molecular cloning, pathologically-correlated expression and functional characterization of the colonystimulating factor 1 receptor (CSF-1R) gene from a teleost, Plecoglossus altivelis check for this species in other resources
CHEN, Qiang; LU, Xin-Jiang; LI, Ming-Yun & CHEN, Jiong

Abstract

Colony-stimulating factor 1 receptor (CSF-1R) is an important regulator of monocytes/macrophages (MO/MΦ). Although several CSF-1R genes have been identified in teleosts, the precise role of CSF- 1R in ayu ( Plecoglossus altivelis check for this species in other resources ) remains unclear. In this study, we characterized the CSF-1R homologue from P. altivelis, and named it PaCSF-1R. Multiple sequence alignment and phylogenetic tree analysis showed that PaCSF-1R was most closely related to that of Japanese ricefish (Oryzias latipes). Tissue distribution and expression analysis showed that the PaCSF-1R transcript was mainly expressed in the head kidney-derived MO/MΦ, spleen, and head kidney, and its expression was significantly altered in various tissues upon Vibrio anguillarum infection. After PaCSF-1R neutralization for 48 h, the phagocytic activity of MO/MΦ was significantly decreased, suggesting that PaCSF-1R plays a role in regulating the phagocytic function of ayu MO/MΦ.

Keywords
Colony-stimulating factor 1 receptor; Pathologically-correlated expression; Monocytes/ macrophages; Phagocytosis; Sequence analysis

 
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