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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 37, No. 4, 2016, pp. 205-213
Bioline Code: zr16031
Full paper language: English
Document type: Review Article
Document available free of charge

Zoological Research, Vol. 37, No. 4, 2016, pp. 205-213

 en Generation of genetically modified mice using CRISPR/Cas9 and haploid embryonic stem cell systems
Jin, Li-Fang & Li, Jin-Song

Abstract

With the development of high-throughput sequencing technology in the post-genomic era, researchers have concentrated their efforts on elucidating the relationships between genes and their corresponding functions. Recently, important progress has been achieved in the generation of genetically modified mice based on CRISPR/Cas9 and haploid embryonic stem cell (haESC) approaches, which provide new platforms for gene function analysis, human disease modeling, and gene therapy. Here, we review the CRISPR/Cas9 and haESC technology for the generation of genetically modified mice and discuss the key challenges in the application of these approaches.

Keywords
CRISPR/Cas9; Haploid embryonic stem cells; Mouse; Genetic modification

 
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