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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 37, No. 5, 2016, pp. 301-306
Bioline Code: zr16045
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 37, No. 5, 2016, pp. 301-306

 en Identification of candidate piRNAs in the gonads of Paralichthys olivaceus check for this species in other resources (Japanese flounder)
Wang, Chun-Lei; Wang, Zhi-Peng; Wang, Jia-Qi & Chen, Xiao-Wu

Abstract

Piwi-interacting RNA (piRNA) plays an important role in the gonadal development and maintenance of Teleostei. In this study, piRNA libraries derived from the adult gonads of Japanese flounder ( Paralichthys olivaceus check for this species in other resources ) were generated using next-generation sequencing technology. Using zebrafish piRNAs as a reference, 5 865 unique candidate piRNAs were identified; 289 candidate piRNA clusters (PRCs) were generated from the above piRNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias, 24 testis-specific, and 131 testis-bias PRCs were found. The relative expression levels of seven PRCs were validated through quantitative reverse transcription-polymerase chain reaction. The results of this study will help facilitate exploration of the development and maintenance of the phenotypic sex mechanism in P. olivaceus.

Keywords
Paralichthys olivaceus; Ovary; Testis; piRNA

 
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