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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 37, No. 5, 2016, pp. 307-312
Bioline Code: zr16046
Full paper language: English
Document type: Report
Document available free of charge

Zoological Research, Vol. 37, No. 5, 2016, pp. 307-312

 en Identification of Sphaeroma terebrans check for this species in other resources via morphology and the mitochondrial cytochrome c oxidase subunit I (COI) gene
Li, Xiu-Feng; Han, Chong; Zhong, Cai-Rong; Xu, Jun-Qiu & Huang, Jian-Rong

Abstract

Sphaeroma terebrans check for this species in other resources , a wood-boring isopoda, is distributed worldwide in tropical and subtropical mangroves. The taxonomy of S. terebrans is usually based on morphological characteristics, with its molecular identification still poorly understood. The number of teeth on the uropodal exopod and the length of the propodus of the seventh pereopod are considered as the major morphological characteristics in S. terebrans, which can cause difficulty in regards to accurate identification. In this study, we identified S. terebrans via molecular and morphological data. Furthermore, the validity of the mitochondrial cytochrome c oxidase subunit I (COI) gene as a DNA barcode for the identification of genus Sphaeroma check for this species in other resources , including species S. terebrans, S. retrolaeve check for this species in other resources , and S. serratum check for this species in other resources , was examined. The mitochondrial COI gene sequences of all specimens were sequenced and analysed. The interspecific Kimura 2-parameter distances were higher than intraspecific distances and no intraspecific-interspecific distance overlaps were observed. In addition, genetic distance and nucleotide diversity (π) exhibited no differences within S. terebrans. Our results revealed that the mitochondrial COI gene can serve as a valid DNA barcode for the identification of S. terebrans. Furthermore, the number of teeth on the uropodal exopod and the length of the propodus of the seventh pereopod were found to be unreliable taxonomic characteristics for S. terebrans.

Keywords
Sphaeroma terebrans; DNA barcode; COI gene; Molecular identification

 
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