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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 38, No. 6, 2017, pp. 449-458
Bioline Code: zr17043
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 38, No. 6, 2017, pp. 449-458

 zh 14种萤火虫(鞘翅目:萤科)基因组大小的测定
Liu, Gui-Chun; Dong, Zhi-Wei; He, Jin-Wu; Zhao, Ruo-Ping; Wang, Wen & Li, Xue-Yan


基因组大小数据是比较基因组进化研究的基础,也是对非模型生物基因组测序成本和难度的很好估计。在本研究中,我们应用流式细胞术估计了14种6属萤火虫的基因组大小(萤亚科Lampyrniae:2个属;熠萤亚科Luciolinae:3属;亚科位置不确定:1属)。我们的研究表明,萤科昆虫单倍体基因组大小在0.42到1.31 pg之间,基因组大小变化为3.1倍。基因组大小在所研究的亚科和属中有很大变异,扁萤属(Lamprigera)具有较大的基因组,而窗萤属( Pyrocoelia check for this species in other resources )具有较小的基因组。在我们的研究中,萤火虫基因组大小与其形态特征(如身体长度、身体宽度、眼睛宽度和触角长度)之间没有相关性。我们的研究提供了更多萤科昆虫基因组大小的数据;此外,研究结果将有助于阐明非模型生物基因组测序的成本和难度,并将有助于促进对萤火虫生物荧光起源的进化基因组学研究。


单倍体基因组大小; 萤火虫; 流式细胞术; 进化

 
 en Genome size of 14 species of fireflies (Insecta, Coleoptera, Lampyridae)
Liu, Gui-Chun; Dong, Zhi-Wei; He, Jin-Wu; Zhao, Ruo-Ping; Wang, Wen & Li, Xue-Yan

Abstract

Eukaryotic genome size data are important both as the basis for comparative research into genome evolution and as estimators of the cost and difficulty of genome sequencing programs for non-model organisms. In this study, the genome size of 14 species of fireflies (Lampyridae) (two genera in Lampyrinae, three genera in Luciolinae, and one genus in subfamily incertae sedis) were estimated by propidium iodide (PI)-based flow cytometry. The haploid genome sizes of Lampyridae ranged from 0. 42 to 1. 31 pg, a 3. 1-fold span. Genome sizes of the fireflies varied within the tested subfamilies and genera. Lamprigera and Pyrocoelia check for this species in other resources species had large and small genome sizes, respectively. No correlation was found between genome size and morphological traits such as body length, body width, eye width, and antennal length. Our data provide additional information on genome size estimation of the firefly family Lampyridae. Furthermore, this study will help clarify the cost and difficulty of genome sequencing programs for non-model organisms and will help promote studies on firefly genome evolution, such as the origin of firefly bioluminescence.

Keywords
Haploid genome size; Firefly; Flow cytometry; Evolution

 
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