search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 39, No. 2, 2018, pp. 58-71
Bioline Code: zr18008
Full paper language: English
Document type: Review Article
Document available free of charge

Zoological Research, Vol. 39, No. 2, 2018, pp. 58-71

 en In vivo genome editing thrives with diversified CRISPR technologies
Ma, Xun; Wong, Avery Sum-Yu; Tam, Hei-Yin; Tsui, Samuel Yung-Kin; Chung, Dittman Lai-Shun & Feng, Bo

Abstract

Prokaryotic type II adaptive immune systems have been developed into the versatile CRISPR technology, which has been widely applied in site-specific genome editing and has revolutionized biomedical research due to its superior efficiency and flexibility. Recent studies have greatly diversified CRISPR technologies by coupling it with various DNA repair mechanisms and targeting strategies. These new advances have significantly expanded the generation of genetically modified animal models, either by including species in which targeted genetic modification could not be achieved previously, or through introducing complex genetic modifications that take multiple steps and cost years to achieve using traditional methods. Herein, we review the recent developments and applications of CRISPR-based technology in generating various animal models, and discuss the everlasting impact of this new progress on biomedical research.

Keywords
CRISPR/Cas9; Genome editing; Animal models

 
© Copyright 2018 - Zoological Research
Alternative site location: http://www.zoores.ac.cn/

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil