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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 40, No. 3, 2019, pp. 205-210
Bioline Code: zr19020
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 40, No. 3, 2019, pp. 205-210

 en Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis
Cheng, Ruo-Mei; Tang, Xiao-Peng; Long, Ai-Lin; Mwangi, James; Lai, Ren; Sun, Rui-Pu; Long, Cheng-Bo & Zhang, Zhen-Qing

Abstract

Protease inhibitors have been reported rarely from the leech Hirudinaria manillensis. In this study, we purified a novel protease inhibitor (bdellin-HM-2) with anticoagulant properties from H. manillensis. With a molecular weight of 1.4x104, bdellin-HM-2 was also characterized with three intra-molecular disulfide bridges at the N-terminus and multiple HHXDD and HXDD motifs at the C-terminus. cDNA cloning revealed that the putative nucleotide-encoding protein of bdellin-HM-2 contained 132 amino acids and was encoded by a 399 bp open reading frame (ORF). Sequence alignment showed that bdellin-HM-2 shared similarity with the “non-classical” Kazal-type serine protease inhibitors, but had no inhibitory effect on trypsin, elastase, chymotrypsin, kallikrein, factor XIIa (FXIIa), factor XIa (FXIa), factor Xa (FXa), thrombin, or plasmin. Bdellin-HM-2 showed anticoagulant effects by prolonging the activated partial thromboplastin time (aPTT), indicating a role in enabling H. manillensis to obtain a blood meal from its host. Our results suggest that bdellin-HM-2 may play a crucial role in blood-sucking in this leech species and may be a potential candidate for the development of clinical anti-thrombotic drugs.

Keywords
Hirudinaria manillensis; Bdellin-HM-2; "Non-classical” Kazal inhibitors; Blood sucking; Anticoagulant; Anti-thrombotic drugs

 
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