The systematics of Semisulcospiridae in China is revised here based on morphological characters and mitochondrial phylogenetics. Phylogenetic relationships within the Chinese semisulcospirids were assessed via DNA sequences from mitochondrial analysis (cytochrome c oxidase I and 16S rRNA). This research contains most morphospecies of semisulcospirids previously recorded in China. Based on these results, the family of Chinese Semisulcospiridae is represented by three genera: i.e., viviparous Semisulcospira
Böttger, 1886, oviparous Hua
Chen, 1943 and Koreoleptoxis
Burch and Jung, 1988. These genera can be distinguished from each other by reproductive anatomy, reproductive mode, and radula features. Species of Hua
are mainly distributed in southwest China and Guangxi, whereas Koreoleptoxis
are mainly distributed in south and northeast China.