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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 41, No. 3, 2020, pp. 281-291
Bioline Code: zr20031
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 41, No. 3, 2020, pp. 281-291

 en Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens
Jiang, Zi-Qin; Wu, Han-Yu; Tian, Jing; Li, Ning & Hu, Xiao-Xiang

Abstract

Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. In this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells (PGCs) to generate transgenic chickens. The lentiviral vectors were pseudotyped with a modified Sindbis virus envelope protein (termed M168) and conjugated with an antibody specific to PGC membrane proteins. We demonstrated that these optimized M168-pseudotyped lentiviral vectors conjugated with SSEA4 antibodies successfully targeted transduction of PGCs in vitro and in vivo. Compared with the control, 50.0%–66.7% of chicken embryos expressed green fluorescent protein (GFP) in gonads transduced by the M168-pseudotyped lentivirus. This improved the targeted transduction efficiency by 30.0%–46.7%. Efficient chimerism of exogenous genes was also observed. This targeting technology could improve the efficiency of germ line transmission and provide greater opportunities for transgenic poultry studies.

Keywords
M168-pseudotyped lentiviral vectors; Primordial germ cells; Targeted transduction; Transgenic chickens; SSEA4

 
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