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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 41, No. 3, 2020, pp. 314-327
Bioline Code: zr20033
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 41, No. 3, 2020, pp. 314-327

 en Novel insights into host-pathogen interactions of large yellow croakers ( Larimichthys crocea check for this species in other resources ) and pathogenic bacterium Pseudomonas plecoglossicida check for this species in other resources using time-resolved dual RNA-seq of infected spleens
Tang, Yi; Xin, Ge; Zhao, Ling-Min; Huang, Li-Xing; Qin, Ying-Xue; Su, Yong-Quan; Zheng, Wei-Qiang; Wu, Bin; Lin, Nan & Yan, Qing-Pi

Abstract

Host-pathogen interactions are highly complex, involving large dynamic changes in gene expression during infection. These interactions are fundamental to understanding anti-infection immunity of hosts, as well as the pathogenesis of pathogens. For bacterial pathogens interacting with animal hosts, time-resolved dual RNA-seq of infected tissue is difficult to perform due to low pathogen load in infected tissue. In this study, an acute infection model of Larimichthys crocea infected by Pseudomonas plecoglossicida was established. The spleens of infected fish exhibited typical symptoms, with a maximum bacterial load at two days post-injection (dpi). Time-resolved dual RNA-seq of infected spleens was successfully applied to study host-pathogen interactions between L. crocea and P. plecoglossicida. The spleens of infected L. crocea were subjected to dual RNA-seq, and transcriptome data were compared with those of noninfected spleens or in vitro cultured bacteria. Results showed that pathogen-host interactions were highly dynamically regulated, with corresponding fluctuations in host and pathogen transcriptomes during infection. The expression levels of many immunogenes involved in cytokine-cytokine receptor, Toll-like receptor signaling, and other immune-related pathways were significantly up-regulated during the infection period. Furthermore, metabolic processes and the use of oxygen in L. crocea were strongly affected by P. plecoglossicida infection. The WGCNA results showed that the metabolic process was strongly related to the entire immune process. For P. plecoglossicida, the expression levels of motility-related genes and flagellum assembly-related genes were significantly up-regulated. The results of this study may help to elucidate the interactions between L. crocea and P. plecoglossicida.

Keywords
Larimichthys crocea; Pseudomonas plecoglossicida; Dual RNA-seq; Host-pathogen interactions; Dynamic transcriptome

 
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