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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 41, No. 4, 2020, pp. 410-422
Bioline Code: zr20043
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 41, No. 4, 2020, pp. 410-422

 en Dual RNA-seq provides novel insight into the roles of dksA from Pseudomonas plecoglossicida check for this species in other resources in pathogen-host interactions with large yellow croakers ( Larimichthys crocea check for this species in other resources )
Wang, Lu-Ying; Liu, Zi-Xu; Zhao, Ling-Min; Huang, Li-Xing; Qin, Ying-Xue; Su, Yong-Quan; Zheng, Wei-Qiang; Wang, Fan & Yan, Qing-Pi


Pseudomonas plecoglossicida is a rod-shaped, gram-negative bacterium with flagella. It causes visceral white spot disease and high mortality in Larimichthys crocea during culture, resulting in serious economic loss. Analysis of transcriptome and quantitative real-time polymerase chain reaction (PCR) data showed that dksA gene expression was significantly up-regulated after 48 h of infection with Epinephelus coioides check for this species in other resources (log 2FC=3.12, P<0.001). RNAi of five shRNAs significantly reduced the expression of dksA in P. plecoglossicida, and the optimal silencing efficiency was 96.23%. Compared with wild-type strains, the symptoms of visceral white spot disease in L. crocea infected with RNAi strains were reduced, with time of death delayed by 48 h and mortality reduced by 25%. The dksA silencing led to a substantial down-regulation in cellular component-, flagellum-, and ribosome assembly-related genes in P. plecoglossicida, and the significant up-regulation of fliC may be a way in which virulence is maintained in P. plecoglossicida. The GO and KEGG results showed that RNAi strain infection in L. crocea led to the down-regulation of inflammatory factor genes in immune-related pathways, which were associated with multiple immune response processes. Results also showed that dksA was a virulence gene in P. plecoglossicida. Compared with the wild-type strains, RNAi strain infection induced a weaker immune response in L. crocea.

Dual RNA-seq; dksA; Pathogen-host interactions; Pseudomonas plecoglossicida; Larimichthys crocea

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