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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 41, No. 5, 2020, pp. 539-551
Bioline Code: zr20057
Full paper language: English
Document type: Research Article
Document available free of charge

Zoological Research, Vol. 41, No. 5, 2020, pp. 539-551

 en Assessment of mitochondrial function in metabolic dysfunction-associated fatty liver disease using obese mouse models
Zhao, Qiong-Ya; Ge, Ling-Hong; Zhang, Kun; Chen, Hai-Feng; Zhan, Xin-Xin; Yang, Yue; Dang, Qing-Lin; Zheng, Yi; Zhou, Huai-Bin; Lyu, Jian-Xin & Fang, He-Zhi

Abstract

Metabolic dysfunction-associated fatty liver disease (MAFLD) is characterized by deregulated hepatic lipid metabolism; however, the association between MAFLD development and mitochondrial dysfunction has yet to be confirmed. Herein, we employed high-resolution respirometry, blue native polyacrylamide gel electrophoresis-based in-gel activity measurement and immunoblot analysis to assess mitochondrial function in obesity-induced mouse models with varying degrees of MAFLD. Results showed a slight but significant decrease in hepatic mitochondrial respiration in some MAFLD mice compared to mice fed a standard diet. However, the activities and levels of mitochondrial oxidative phosphorylation complexes remained unchanged during obesity-induced MAFLD progression. These results suggest that mitochondrial function, particularly oxidative phosphorylation, was mildly affected during obesity-induced MAFLD development. Moreover, transcriptome profiling of mouse and human liver tissues with varying degrees of MAFLD revealed that the decreased activation of mitochondria-related pathways was only associated with MAFLD of a high histological grade, whereas the major regulators of mitochondrial biogenesis were not altered in mice or humans during MAFLD development. Collectively, our results suggest that impaired hepatic mitochondrial function is not closely associated with obesity-induced MAFLD. Therefore, therapeutic strategies targeting mitochondria for the treatment of MAFLD should be reconsidered.

Keywords
Obesity; Mitochondria; Metabolic dysfunction-associated fatty liver disease; Hepatic steatosis; Steatohepatitis

 
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