An experiment was carried out in order to study, in laboratory condition, the pathogenicity of
Beauveria brongniartii
(strain LF-05)
against
Musca domestica
. The conidia of
B. brongniartii were obtained from Perú.
The fungus was propagated in potato-dextrose-agar culture medium, and later in semi-crude sterilized rice. F
1
flies were obtained from wild specimens which were captured with mesh in a poultry breeding farm (Betijoque,
Trujillo state, Venezuela). In the laboratory, one hundred and sixty adult flies from 3 days of age, divided into
8 lots with 17 to 40 per bottle flies were anesthetized with ether, and then applied
B. brongniartii suspensions
diluted in water from 10
8
conidia/mL, resulting in sis treatments: control, 1.2 x 10
3
, 1.2 x 10
4
, 1.2 x 10
5
, 1.2 x
10
6
, and 1.2 x 10
7
conidia/mL. Healthy controls with 100 flies were used with only ether as anesthesia. The
counting of fallen flies, both in the experimental and bottles at checkpoints, it was up to 24 days. Using the
Probit® methodology, it was found that the LT
50
and LT
95
were 11.08 and 13.25 days earlier than control group,
respectively. Results showed that spores of
B. brongniartii (strain LF-05), at 1.2 x107
conidia/mL, resulted in
95% mortality of
M. domestica in 9.27 days. The use of nebulizations with similar concentration of spores to fly
control in local production units of poultry and cattle were speculated.