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Zootecnia Tropical
Instituto Nacional de Investigaciones Agrícolas Venezuela
ISSN: 0798-7269
Vol. 28, No. 1, 2010, pp. 83-91
Bioline Code: zt10011
Full paper language: English
Document type: Research Article
Document available free of charge

Zootecnia Tropical, Vol. 28, No. 1, 2010, pp. 83-91

 es
Pineda, Juan; Principal, Judith; Barrios, Carlos; Milla, Deivis; Solano, Yohan & Gil, Elizabeth

Resumen

La antracnosis causada por el hongo Colletotrichum gloeosporioides check for this species in other resources , es una patología que ocasiona pudriciones postcosecha y reduce el valor comercial de muchas frutas en Venezuela. Con el propósito de evaluar la acción fungistática del propóleos de Apis mellifera check for this species in other resources sobre cepas de C. gloeosporioides que afecta frutos de aguacate ( Persea americana check for this species in other resources ), lechosa ( Carica papaya check for this species in other resources ) y parchita ( Passiflora edulis check for this species in other resources ), se utilizó un propóleos proveniente de la Estación de Apicultura, Sub-estación Guaremal, UCLA. Los tratamientos fueron: Testigo (sin tratar) y diluciones del propóleos en etanol al 0%, 15%, 20% y 30%. En medio de cultivo Agar-Papa-Dextrosa (APD), se colocó en cada cápsula 4 círculos de papel filtro, previamente remojados durante 5 minutos en el propóleos a la dilución necesaria y un disco de la cepa respectiva de C. gloeosporioides en el centro de la misma. Las mediciones se realizaron diariamente hasta que el testigo alcanzó el máximo radio. En la dilución se determinó la presencia de metabolitos secundarios. Se observaron diferencias significativas entre los tratamientos, al compararlos con el testigo, el menor desarrollo del hongo ocurrió, para las cepas provenientes de aguacate y parchita, con propóleos al 30% (27,6 mm y 28,9 mm, respectivamente), y para la de lechosa, con propóleos al 20% (25,3 mm). La inhibición del crecimiento estuvo alrededor del 30%. Las diluciones del propóleos al 15%, 20% y 30% estadísticamente no presentaron diferencias significativas entre sí, por lo cual resultaron similares en su efecto. Se demostró el efecto supresor del propóleos sobre el crecimiento micelial de C. gloeosporioides y la presencia de compuestos flavonoides en el extracto.

Palabras-clave
antracnosis, Colletotrichum gloeosporioides, propóleos, fungistático

 
 en In vitro fungistatic property of propolis on three Colletotrichum gloeosporioides check for this species in other resources isolates
Pineda, Juan; Principal, Judith; Barrios, Carlos; Milla, Deivis; Solano, Yohan & Gil, Elizabeth

Abstract

Antracnose caused by the fungus Colletotrichum gloeosporioides, check for this species in other resources is a pathology that causing fruit rot and reducing the commercial value of many fruits in Venezuela. In order to evaluate the antifungic property of Apis mellifera check for this species in other resources propolis on isolates of C. gloeosporioides that affect fruits of avocado ( Persea americana check for this species in other resources ), papaya ( Carica papaya check for this species in other resources ) and passion fruit ( Passiflora edulis check for this species in other resources ), a study was done using a propolis collected from the Guaremal Apicultural Station, UCLA. The treatments were: control (no treated) and dilutions of the propolis in ethanol to 0%, 15%, 20% and 30%. On PDA culture medium, was placing in each Petri dish four circles of paper filter, previously soaked during 5 minutes in the propolis to the necessary dilution, and a disc of the respective strain of C. gloeosporioides in the center of the same one. The measurements were made daily until the control treatment reached the maximum ratio. In the propolis dilution the presence of secondary metabolites was determined. Significant differences between treatments were observed as far as development of the fungus, when comparing them with control treatment, the smaller development of the fungus happened, for the stocks of avocado and passion fruit, with propolis to 30% (27.6 mm and 28.9 mm, respectively), and for the one of papaya, with propolis to 20% (25.3 mm). The inhibition of the growth was around 30%. The propolis dilutions at 15, 20 and 30% statistically did not present significant differences to each other, thus those were similar in their effect. A suppressor effect of the propolis on the micelial growth of C. gloeosporioides and presence of flavonoids compounds in extract were demonstrated.

Keywords
antracnose, Colletotrichum gloeosporioides, honeybee propolis, fungistatic.

 
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