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Zootecnia Tropical
Instituto Nacional de Investigaciones Agrícolas Venezuela
ISSN: 0798-7269
Vol. 30, No. 1, 2012, pp. 9-15
Bioline Code: zt12002
Full paper language: English
Document type: Research Article
Document available free of charge

Zootecnia Tropical, Vol. 30, No. 1, 2012, pp. 9-15

 es Reactivación de un cepario de Babesia bigemina check for this species in other resources , Babesia bovis check for this species in other resources y Anaplasma marginale check for this species in other resources , para estudios experimentales
Madrid, Carmen; Fuentes, Henrry; Romero, Williams; Álvarez, Ana & Espinoza, Emir

Resumen

El uso de microorganismos conservados ( Babesia check for this species in other resources sp y Anaplasma check for this species in other resources sp) son claves en la solución de los graves problemas generados por los hemotrópicos de interés veterinario en Venezuela. Aislados de Anaplasma marginale check for this species in other resources , Babesia bigemina check for this species in other resources y Babesia bovis check for this species in other resources criopreservados en nitrógeno líquido a (− 196)°C por largo tiempo, se inocularon en becerros esplenectomizados, con el objeto de evaluar la viabilidad de mismos. Los resultados mostraron alta patogenidad de los aislados, con manifestaciones clínicas de fi ebre, anorexia, incoordinación (B. bovis) y hemoglobinuria (B. bigemina). La virulencia de los hemoparásitos medida por la disminución del hematocrito, indicó mayor severidad para los aislados de B. bigemina y A. marginale. La criopreservación de estos parásitos permitirá la producción de antígenos en cantidad y calidad para análisis de rutina de estas enfermedades de interés veterinario, desde el punto de vista parasitológico, serológico y de biología molecular.

Palabras-clave
Reactivación, viabilidad, Anaplasma marginale, Babesia sp.

 
 en Reactivaction of parasites stock Babesia bigemina check for this species in other resources , Babesia bovis check for this species in other resources y Anaplasma marginale check for this species in other resources , for experimental studies
Madrid, Carmen; Fuentes, Henrry; Romero, Williams; Álvarez, Ana & Espinoza, Emir

Abstract

The uses of conserved microorganisms ( Babesia check for this species in other resources sp and Anaplasma check for this species in other resources sp) are keys in the solution of serious problems generated by veterinary haemotropic parasites of serious concern in Venezuela. Isolated Anaplasma marginale check for this species in other resources , Babesia bigemina check for this species in other resources and Babesia bovis check for this species in other resources cryopreserved in liquid nitrogen at (− 196)°C long splenectomised calves were inoculated with the object of assessing their viability. Results showed high pathogenecity of isolates, with clinical manifestations of fever, anorexia, incoordination (B. bovis) and haemoglobinuria (B. bigemina). The virulence of the hemoparasites measured by the decrease of hematocrit indicated greater severity for isolates of B. bigemina and A. marginale. Cryopreservation of these parasites allows antigen production in quantity and quality for routine analysis of these diseases of veterinarian interest, from the viewpoint parasitological, serological and molecular biology.

Keywords
Reactivaction, viability, Anaplasma marginale, Babesia sp.

 
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