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Zootecnia Tropical
Instituto Nacional de Investigaciones Agrícolas Venezuela
ISSN: 0798-7269
Vol. 30, No. 1, 2012, pp. 109-114
Bioline Code: zt12012
Full paper language: English
Document type: Note
Document available free of charge

Zootecnia Tropical, Vol. 30, No. 1, 2012, pp. 109-114

 en Molecular evidence of transplacental transmission of Anaplasma marginale check for this species in other resources in zebu neonatal calves of Venezuela
Meldonado, Jesús; Coronado, Alfredo; Kowalski, Andrés & Medina, Juan

Abstract

Anaplasma marginale check for this species in other resources is the most prevalent protobacteria in cattle worldwide. This pathogen multiplies in the erythrocytes and endothelium causing a hemolytic disease called anaplasmosis, which in its acute phase is accompanied by anemia, weight loss and even death. It is assumed that the organism is transmitted biologically by ixodides, mechanically by haematophagous dipterans, iatrogenically or by transplacental transmission. In order to detect neonatal anaplasmosis in a Brahman herd of the Yaracuy State, Venezuela, blood samples were collected from 29 calves less than 24 hours old and their postpartum mothers. The DNA obtained from 58 samples was subjected to a nested PCR (nPCR) specifi c for the diagnosis of Anaplasma spp. In addition the serum of the 29 mothers was evaluated by a competitive ELISA (ELISAc) for diagnosis of anaplasmosis using the protocol indicated by the commercial house VMRD. The results showed that in 20.69% of the offspring (6 calves) gene amplifi cation was obtained from msp5 of Anaplasma spp, while all the samples from the mothers were positive for nPCR and ELISAc. The experimental evidence obtained indicates that the presence of protobacteria in animals less than 24 hours of birth is the result of transplacental transmission from the infected mother to the fetus during pregnancy and shows that under the studied conditions this route of transmission is important in the spread of the pathogen.

Keywords
Neonatal anaplasmosis, Anaplasma marginale, nested PCR, competitive ELISA, msp5, transplacental transmission.

 
 es Evidencia molecular de transmisión transplacentaria de Anaplasma marginale check for this species in other resources en becerros neonatos cebú de Venezuela
Meldonado, Jesús; Coronado, Alfredo; Kowalski, Andrés & Medina, Juan

Resumen

Anaplasma marginale check for this species in other resources es la protobacteria de mayor prevalencia en el ganado bovino a nivel mundial. Este patógeno se multiplica en los eritrocitos y endotelio causando la enfermedad hemolítica denominada anaplasmosis, que en su fase aguda se acompaña de anemia, pérdida de peso e incluso la muerte. Se asume que el microorganismo es transmitido biológicamente por ixódidos, mecánicamente por dípteros hematófagos, iatrogénicamente o por vía transplacentaria. Con el fi n de detectar la presencia de A. marginale en becerros neonatos de un rebaño Brahman del estado Yaracuy, Venezuela, se colectaron muestras de sangre de 29 crías con menos de 24 horas de nacidas y sus correspondientes madres recién paridas. El ADN obtenido de las 58 muestras fue sometido a una PCR anidada (PCRa) específi ca para el diagnóstico de Anaplasma spp. Adicionalmente a los sueros de las 29 madres se les realizó un ELISA competitivo (ELISAc) para el diagnóstico de anaplasmosis utilizando el protocolo señalado por la casa comercial VMRD. Los resultados mostraron que en el 20,69% de las crías (6 neonatos) se obtuvo amplifi cación del gen msp5 de Anaplasma spp, mientras que todas las muestras de las madres eran positivas a la PCRa y al ELISAc. La evidencia experimental obtenida indica que la presencia de la protobacteria en animales con menos de 24 horas de nacidos es producto de la transmisión transplacentaria de la madre infectada al feto durante la gestación y pone de manifi esto que bajo las condiciones estudiadas esta vía de transmisión es importante en la diseminación del patógeno.

Palabras-clave
Anaplasmosis neonatal, Anaplasma marginale, PCR anidado, ELISA competitivo, msp5, transmisión transplacentaria.

 
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Alternative site location: http://www.sian.inia.gob.ve/repositorio/revistas_ci/ZootecniaTropical/ztindice.htm

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