SACCHAROMONOSPORA YANGSHUOENSIS SP. NOV.
H. RUNMAO, W. GUIZHEN and L. JUNYING
New Antibiotics Res. Dept., Sichuan Industrial Institute of
Antibiotics, Sichuan, Chengdu, China
Code Number: AC94002
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ABSTRACT. A new species of Saccharomonospora (S. yangshuoensis)
is described. The organism is characterised by single
spores, mainly on the aerial mycelium, and good growth on ISP 5
with vitamins, but poor growth on the unsupplemented ISP5 medium.
Growth on calcium malate medium produces a deep brownish
pigmentation. The type strain of the species is NA 919594.
During an investigation of the actinomycete population in Guangxi
province, seven strains were isolated from a soil sample collected
in Yangshuo, P.R. China. These strains belong to the genus
Saccharomonospora, as determined by their morphological
features and cell wall composition. In the present report strain NA
919594 is compared with previously identified species of
Saccharomonospora.
MATERIALS and METHODS
Microorganisms. Strain NA 919594 was isolated in September
1991 from a soil sample collected in Yangshuo, Guangxi province,
P.R.China. For comparative purposes Saccharomonospora azurea,
strain NA 128 (ATCC 43570) and S. cyanea, strain NA 134
(ATCC 43724) were also studied.
Morphological characteristics. Observations were
carried out by light microscopy on cultures grown at 37 C for 2wks
on ISP5 medium supplemented with 1.0ml of a vitamin solution
(thiamine-HCl, 0.5mg; riboflavin, 0.5mg; niacin, 0.5mg;
Ca-pantothenate, 0.5mg; p-aminobenzoic-acid, 0.5mg; pyridoxinHCl,
0.5mg; inositol, 0.5mg; biotin, 0.25mg; dist. water, 100ml).
Spore morphology was examined by scanning electron microscopy
(SEM).
Cultural and physiological characteristics. The media
used were those described by Shirling and Gottlieb (1966) and
Waksman (1961), together with the modified ISP5 medium. Cultures
were incubated at 28 and 37 C for two weeks.
Cell wall and whole-cell analysis was performed according to
Becker et al. (1965) and Lechevalier (1968).
RESULTS
Morphology. The vegetative mycelium is irregularly branched
and non fragmenting. Aerial filaments (0.3-0.5um) are branched
monopodially.
Figure 1. SEM of aerial spores of strain NA 919594 on
modified ISP5 (after 3 wks at 37 C).
Spores (Fig. 1), roundish (0.8-1.0um) and with a warty surface, are
sessile and formed singly on aerial hyphae, less frequently on the
substrate mycelium .
Cultural characteristics. These are summarised in Table
1.
Physiological characteristics. Positive gelatine
liquefaction and milk peptonisation. The strain does not grow in
media containing NaCl (3%o). The temperature range for growth is 24
to 40 C with optimum growth occurring at 37 C.
Chemical analysis. Meso-diaminopimelic acid, galactose
and arabinose are present.
Comparison of strains. Five species of
Saccharomonospora (S. azurea, S. cyanea, S. internatus, S.
viridis and S. yunnanensis) have been previously
described (Runmao, 1987, 1988; Agre et al., 1974; Nonomura
and Ohara, 1971; Chenglin and Lihua, 1985).
Differences from strain NA 919594 are as follows: (i) growth
of S. viridis is poor or absent on oatmeal (ISP3) and Czapek
sucrose agar respectively, (ii) S. incarnatus does not grow
on the latter medium, whereas strain NA 919594 shows excellent
growth on both with blue aerial mycelium, (iii) S. yunnanensis
forms spores with warty surfaces and produces a light
yellowish-brown diffusible pigment on nutrient agar, whereas strain
NA 919594 also shows warty spore surfaces, but forms no pigment on
the same medium, (iv) growth of S. azurea and S. cyanea
is poor to moderate on ISP5 and on calcium malate
medium; strain NA 919594 shows poor or no growth on ISP5 and
moderate growth on calcium malate medium with a dark brownish
soluble pigment.
On the basis of these observations it is suggested that strain
NA 919594 represents a new species of the genus
Saccharomonospora. NA 919594 is the type strain and a
culture has been deposited in the Culture Collection Centre of the
Sichuan Industrial Institute of Antibiotics (SIIA), Chengdu,
P.R.China, as strain SIIA 91100.
Description of Saccharomonospora yangshuoensis sp.nov.
Yang.shuo.en'sis. ML. adj. yangshuoensis, pertaining to the
city of Yangshuo (South China), the source of the soil from which
the organism was isolated.
Aerobic actinomycete. Non fragmenting substrate mycelium. No
sporangia formed. Single spores, mainly on aerial mycelium. Spores
oval to roundish, 0.8 - 01um, formed on very short sporophores or
sessile.
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MEDIUM VEGETATIVE AERIAL SOLUBLE
MYCELIUM MYCELIUM PIGMENT
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ISP4 poor growth, hyaline traces, greyish
ISP5 poor growth traces
ISP5 + good growth, hyaline good, whitish
B vitamins
ISP6 good growth, dark good, whitish black
brown melanoid
ISP7 good growth, hyaline good, light blue
Czapek-Dox moderate growth, moderate, azure
hyaline
Nutrient agar good growth, light good, blue
brown
Calcium moderate growth, moderate, whitish
dark malate dark brown
brown
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Table 1. Cultural characteristics of Saccharomonospora
yangshuoensis, strain NA 919594.
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Spore surface warty. Aerial mycelium whitish or pale azure on
oatmeal and Czapek sucrose media. No growth (or poor) on ISP5, ISP6
or ISP7 media. Excellent growth on vitamin supplemented ISP5
medium. Dark brown soluble pigment formed on calcium malate
medium.
Mesophilic.
Habitat: soil.
Type strain: NA 919594 (SIIA 91100).
ACKNOWLEDGEMENTS. The authors wish to thank Dr.Chen Weiqun of the
Central Analysis Department of Sichuan University for electron
microscopy, Mr. Mu Lianjun and Mr. Wenbin for collecting the soil
sample and Prof Cai Shun-yang for advice and assistance.
REFERENCES
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Chenglin, J. & X. Lihua (1985). A study on aquatic
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Copyright 1994 C. E. T A.
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