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Actinomycetes
University of Udine, Mycology Department
ISSN: 0732-0574
Vol. 8, Num. 3, 1997
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Actinomycetes, 1997, Vol.8, Part 3. pp.66-69
ACTINOMYCETOLOGICA
Vol.11, No. 1, 1997
Published by the Society for Actinomycetes, Japan
ABSTRACTS OF PAPERS
Code Number: AC97011
Sizes of Files:
Text: 6K
Graphics: No associated graphics files
PROTOPLASTING AND REGENERATION OF STRAINS BELONGING TO THE GENUS
ACTINOMADURA
T. Dairi, Y. Hamano, Y. Igarashi, T. Furumai and T. Oki
Toyanna Prefectural University, Biotechnology Research Center, 5180,
Kurokawa, Kosugi, Toyanna 939-03, Japan
Actinomycetologica, 11: 1-5, 1997
A reproducible and reliable protoplasting and regeneration condition for
the genus Actinomadura strains, which is extraordinarily resistant
to lysozyme, has been studied. Protoplasts were prepared from all strains
examined by use of both lysozyme and N-acetylmuramidase only when they were
grown in the presence of sucrose (10-30 %) and glycine (0.3 %). The optimum
concentration of sucrose was strain-dependent. Protoplasts could regenerate
on the modified RS medium when the sucrose concentration was the same as
that of culture medium used. Thus established regeneration medium provided
us with regeneration ratios of 0.4 to 30%.
Authors' Abstract
IDENTIFICATION OF STRAIN MF861-C4, A KASUGAMYCIN-PRODUCING
ACTINOMYCETE, AS STREPTOMYCES ALBULUS
N. Kinoshita, S. Hattori, S. Ikeno^1, M. Hori^1 and M. Hamada
Institute of Microbial Chemistry, 14-23, Kamiosaki 3-chome, Shinagawa-ku,
Tokyo 141 and
^1 Showa College of Pharmaceutical Sciences, 3-3165 Higashi,
Tarnagawagakuen, Machida-shi, Tokyo 194, Japan
Actinomycetologica, 11: 6-10, 1997
We isolated a kasugamycin-producing actinomycete, strain MF861-C4, which
had spiny spore surface. This strain was different from Streptomyces
kasagaensis but rather close to Streptomyces albulus with regard
to morphological, cultural, and physiological characteristics, DNA homology
and partial base sequences of 16S ribosomal RNA. The strain MF861-C4 was
identified as S. albulus.
Authors' Abstract
FERMENTATION CONDITIONS FOR PANOSE PRODUCTION BY A MUTANT OF
STREPTOMYCES HYGROSCOPICUS SUBSP. LIMONEUS
M.M. Hoquet and E. Higashide^TM
Division of Bioresource Science, The Graduate School of Natural Science and
Technology, and
^1 Department of Agricultural Science, Faculty of Agriculture, Okayama
University, 1-1-1, Tsushimanaka, Okayama-shi 700, Japan
Actinomycetologica, 11: 11-14, 1997
Effect of some inorganic salts, carbon and nitrogen sources on panose
production in the former medium by strain GT-32 was investigated. In the
medium when 0.3% MnSO4.4H2O was added after removing NH4Cl and NaCl, the
strain gave a higher panose production (relative productivity; around (10%)
than that of the former medium studied. With the optimum fermentation
conditions for panose production, the highest production titre (9.8 mg/ml)
was given in 5 days cultured broth.
Authors' Abstract
16S rRNA GENE SEQUENCE ANALYSIS OF STREPTOMYCES LAVENDULAE
AND STREPTOMYCES VIRGINIAE STRAINS
Y. Anzai and J. Watanabe
Nippon Roche Research Center, 200 Kajiwara, Kamakura, Kanagawa 247,
Japan
Actinomycetologica, 11: 15-19, 1997
The 16S rRNA gene sequences of 18 strains of Streptomyces lavendulae
and Streptomyces virginiae were determined. Fifteen strains
including the type strains of S. lavendulae and S. virginiae
had 1,514 nucleotides in positions 10 to 1,541 of the Escherichia
coli numbering system, and their similarities were more than 98.1%. On
the other hand the sequences of S. lavendulae subsp. lavendulae
IFO 3361, S. lavendulae subsp. lavendulae IFO 12341 and
S. virginiae ATCC 13013 consisted of 1,516 nucleotides. The
differences in the number of nucleotides were observed between 3 strains
containing 1,516 nucleotides and 15 strains containing 1,514 nucleotides in
positions 181 to 219, 1,133 to 1,141 and 1,446 to 1,456 (E. coli
numbering system) and various sequence lengths of these areas were
corresponded to the similarity level of the 16S rRNA gene sequence. The
homology values of DNA-DNA hybridization between these three strains and
their type strains were very low (15 to 22%) and these three strains were
located in a cluster far from the other strains in the cluster analysis
based on the taxonomic characteristics (Anzai, Y., T. Okuda & J.Watanabe,
J. Antibiot., 47: 183-193, 1994). Therefore, we conclude that S.
lavendulae subsp. lavendulae IFO 3361 and IFO 12341, and S.
virginiae ATCC 13013 should not be assigned to S. lavendulae or
S. virginiae.
Authors' Abstract
NEW MICROORGANISMS WHICH CONVERT COMPACTIN TO PRAVASTATIN
J. Yashphet, J. Davis, Y. Peng, S.H. Bok^1 and A.L. Demain
Fermentation Microbiology Laboratory, Biology Department, Massachusetts
Institute of Technology, Cambridge, MA 02139, USA and
^1 Genetic Engineering Research Institute, Korean Institute of Science and
Technology, Taejon, Korea
Actinomycetologica, 11: 20-25, 1997
Screening of known and unidentified actinomycetes revealed three strains
which can hydroxylate compactin to pravastatin. They showed taxonomic
properties which are consistent with those of Actinomadura sp.,
Streptomyces tanashiensis and Streptomyces anulatus. Actinomadura
sp. strain 2966 effected a 60% bioconversion of added compactin.
Authors' Abstract
Copyright 1997 C.E.T.A., The International Centre for Theoretical and
Applied Ecology, Gorizia
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