search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Biokemistri
Nigerian Society for Experimental Biology
ISSN: 0795-8080
Vol. 20, Num. 2, 2008, pp. 41-46

Biokemistri, Vol. 20, No. 2, Dec, 2008, pp. 41-46

Investigation on batch biosorption of lead using Lactobacillius bulgaricus in an aqueous phase system 

Mostafa Ghasemi1, Mostafa Rahimnejad1, Ghasem D. Najafpour1*, Mehdi Sedighi2, Mostafa Asadi3 and Babak Hashemiyeh3

1 School of Chemical Engineering, Noushirvani University of Technology, Babol, Iran
2 School of Chemical Engineering, Tarbiat Modares University, Tehran, Iran
3 School of Environmental Engineering, Noushirvani University of Technology, Babol, Iran
*To whom correspondence should be addressed.  E-mail:najafpour@nit.ac.ir, Tel. /fax:+(98) 111-323 4204

Received 20 September 2008

Code Number: bk08007  

Abstract

In this research, the biosorption of lead by Lactobacillus bulgaricus was investigated. The mechanism for the biosorption was similar to ionic exchanger. The media pH, weight of dried biomass and initial lead concentration were investigated at ambient temperature. At high acidic solution (pH<1.5) and also in alkaline condition, there was no significant absorption. In these experiments the highest biosorption of lead occurred at pH value of 6 and the biomass concentration of 4.5 g/l. The removal efficiency of 88.8 percent was obtained with initial lead concentration of 50 mg/l. It was found that Lactobacillus bulgaricus was highly efficient in low ion concentration (less than 100ppm), however for the lead concentration higher than 100ppm the percent of removal was low (less than 50 percent).

Keywords:Lactobacillus bulgaricus, biosorption, lead, dried biomass

INTRODUCTION

The advanced technologies for the industrialization and urbanization have substantially enhanced the degradation of our aquatic environment through the discharge of industrial waste waters and domestic wastes1,2. Heavy metal pollution has become one of the serious environmental problems of worldwide concern because of their extreme toxicity and tendency for bioaccumulation in the food chain even in relatively low concentrations3,4.

The toxic heavy metals cause serious threat to the environment, animals and humans5. In 2000, the United States Environmental Protection Agency (USEPA) prepared a list of organic and inorganic pollutants found in wastewater and which constitute serious health hazards6. Lead is one of the metals on the list and it is extremely toxic to organisms even at low concentration. It can damage the nervous system, gastrointestinal track, encephalopathy with pretreatment damage, kidneys and reproductive system particularly in children1,7.

Exact determination of heavy metal ions in the environmental samples by instrumental techniques like graphite or flame atomic absorption spectroscopy and inductively coupled plasma optical emission spectroscopy is an important in the field of analytical chemistry8. Various processes and measurements such as chemical precipitation, ion exchange, membrane separation and activated carbon adsorption have been employed to remove metal pollutants from aqueous solutions9,10. However most of these methods suffer from some disadvantages including incomplete metal removal, toxic sludge generation and high costs when applied to dilute effluents on a large scale. These lead to a search for cheaper, easily obtainable materials for adsorption of heavy metals or organic pollutants11,12.

Biosorption is a rapid, reversible, economical and environmentally friendly technology in contrast to traditional methods used for removal of heavy metals from streams13-16. The mechanism of binding by inactivated biomass may depend on the chemical nature of pollutant (species, size and ionic charge), type of biomass, its preparation and its specific surface properties and environmental conditions (pH, temperature, ionic strength, existence of competing organic or inorganic ligand in solution). Although biosorption is generally used for the treatment of heavy metal pollutants for wastewaters, it can also be considered a promising technology for the removal of organics from industrial waste streams and polluted natural waters17-19.

The objective of this work was to investigate the effect of initial concentration of lead, pH and biomass concentration on the biosorption of lead using Lactobacillius bulgaricus as biomass.

MATERIALS AND METHODS

Lactobacillus bulgaricus was supplied by Biotechnology Research Center, Noshirvani University of Technology, Babol, Iran. The organism was originally isolated from whey. The microorganism was grown aerobically in an agitated vessel (100 rpm). The medium consisted of 2g/l yeast extract, 20g/l glucose, 0.2g/l K2HPO4, 0.2 g/l MgSO4 and 0.05 g/l MnSO4. The medium was sterilized, autoclaved at 15psig and temperature of 121°C for 20 min. The pH of medium was initially adjusted at 6.5 and the inoclum was introduced after which the culture was incubated at 30°C for 48 hours and the biomass was harvested at maximum cell growth. The filtration process was accomplished using cellulose acetate filter with pore size of 0.45 μm (Sartorius Biotech, Germany). The harvested cells were washed with deionized water to improve the metal binding properties and the biomass was dried at 105°C in an oven for 24 hours20.Finally, the biomass was milled and mechanically sieved to give uniform particles in the range of 250-800µm.

All chemicals and reagents used for the experiments were analytical grades and supplied by Merck (Germany). The pH meter, HANA 211(Romania) model glass-electrode was employed for measuring pH values in the aqueous phase. The initial pH of the working solutions was adjusted by addition of HNO3 or NaOH solution, except for the experiment examining the effect of pH to be active. Fresh dilution was used for each set of the sorption experiments. The stock solution of Pb2+ (1 g/l) was prepared by dissolving Pb(NO3)2 in deionized water.

Batch biosorption experiments were carried out in flasks at 120rpm for the period of contact time using a heating stirrer (VELP Scientifica, Italy). The metal ion concentration was measured in triplicate by flame atomic absorption spectrophotometer (Philips, PU 9400, USA).

Scanning electron microscope

Scanning electron microscope (SEM)was used to show the texture and exposure surface of the dried biomass Lactobacillius bulgaricus. The samples were dried by liquefied nitrogen, coated with gold and observed with a (Phillips XL30, Holland) microscope.  Finally, image of the samples were taken under SEM at x10000 magnification21.

RESULTS AND DISCUSSION

The morphology of the dried biomass Lactobacillius bulgaricusexamined by SEM is presented in Figure 1. The obtained SEM image demonstrated Lactobacillius bulgaricusbiomass was cylindrical in shape. The created surface had great potential to adsorb heavy metals.

The pH is one of the influential parameters which affect biosorption capacity of the heavy metals22,23. It is well known that at strong acidic conditions (pH of less than 1.5) nearly no lead adsorption is observed24. Acidity pH of the aqueous system also influenced the capacity of the sorbent to take up the heavy metal ions, because pH is known to affect the solution chemistry of the metal ions when they are hydrolyzed. Hydrolyzed metal ions tend to form complex organic or inorganic ligands during hydrolysis and condensation reaction catalyzed by an acid or base. 

Figure 2 presents the effect of pH on biosorption capacity. As the pH increased in the solution, higher uptake of lead occurred. When the pH was increased from 1 to 6, the biosorption capacity was increased from zero to 42.6 mg/g for the initial lead concentration of 50 mg/l. The removal efficiency of lead with respect to pH is shown in Figure 3. At pH of 6, an 85.2 % biosorption of the intial concentration of lead was achieved.

At alkaline pH of 8 and higher, the lead ions had precipitated (data not shown). The best pH range for biosorption occurred at 4-6. There was strong resistance against the metal ion adsorbed to the biomass surface probably because functional groups in biomass were highly protonated at strong acid condition since negatively charged groups at biosorbent surface is necessary for the sorption process25. Such sites were unavailable due to competition between Pb2+ and H3O+ ions when the pH is less than 1.5. When the acidity decreased in the solution, the deprotonation of acid functional groups, such as carboxyl, phosphonate and phosphodiester, were strengthened22-25.

The effect of biomass concentration on lead ion removal was also experimented. A small amount of biomass had the potential to remove the heavy metals from an industrial effluent. In order to use minimum amount of biomass for the maximal removal of lead, different weight of biomass from 2 to 5 g/l were used.

Results in Figure 4 show that the maximum biosorption capacity occurred at biomass concentration of 4.5g/l. 

The percentages of the lead removal by biomass concentration of 2 to 5g/l with an increment of 0.5g/l were 66.0, 70.6, 74.6, 76.6, 85.2, 88.8 and 82.4, respectively. The data are presented in Figure 5. By increasing the concentration of the biomass, the lead ion removal was increased.

Additional biomass more than 4.5 g/l may decrease the heavy metal removal.

A set of experiments conducted with lead concentration, in the range of 20, 70, 120, 170, 220 mg/l were prepared to determine the biosorption data at optimum condition (pH of 6 and dried biomass concentration of 4.5 g/l). The results are shown in Figure 6.

The lead uptake was increased with the initial metal ion concentration. Ironically, it was found that the sorption process was active in early stage and most of the lead ion concentrations were absorbed within 30 minutes. 

Figure 7 shows the percentage of lead removal at various lead ion concentrations. The lead removal at low ionic concentration was initially increased till 50 mg/l. Clearly, the percentage of removal was decreased at high lead ion concentrations.

The results show that Lactobacillius bulgaricus biomass was an interesting biomaterial with a low cost biosorbent for the lead removal from an aqueous solution, especially at low ionic concentration of about 50mg/l. The biosorption process was pH dependent, as the pH increased from 2 to 6 the lead removal was increased from 54.4 to 85.2 percent, respectively.

The removal efficiency was also increased with an increase of biomass concentration of 4.5 g/l. The metal ion concentration increased from 20 to 220mg/l, the removal was decreased. There was no biosorption at highly acidic and alkaline conditions. At near neutral condition, the biosorption showed to be effective.

We conclude that biosoption of lead at concentration of 50 mg/l by Lactobacillus bulgaricus at biomass concentration of 4.5 g/l is fastest at pH 6 as the efficiency of removal of lead was 85.2% and we suggest that Lactobacillus bulgaricus can be useful for biosorption of lead from an aqueous system

ACKNOWLEDGMENTS

The authors wish to acknowledge Biotechnology Research Center, Noshirvani University of Technology, Babol, Iran for the facilities provided to accomplish this research. 

REFERENCES 

  1. Senthilkumar, S., Bharathi S. and Nithyanandhi, D. (2000) Biosorption of toxic heavy metals from aqueous solution. Biores. Technol. 75:163-165.
  2. Tunali, S. and Tamer A. (2006) Zn(II) biosorption properties of Botrytis cinerea biomass. J. Haz. Mat. 131:137-145.
  3. Mohan, D. and Singth, K. P. (2002) Single and multi-component adsorption of cadmium and zinc using activated carbon derived from baggase–an agricultural waste. Wat. Res. 36:2304-2318.
  4. Viraraghavan, T. and Rao, G.A.K. (1991) adsorption of cadmium and chromium from wastewater by fly ash. J. Environ. Sci. Hlth. 26:721-753.
  5. Goyal, N., Jian, S. C. and Banerjee, U. C. (2003) Comparative studies on the microbial adsorption of heavy metals. Adv. Environ. Res. 7:311-319.
  6. Ramos, R. L., Cacome, L. A. B., Barron, G. N. and Rubio, L. S (2002) Adsorption of zinc(II) from and aqueous solution on to activated carbon. J. Haz. Mat. 90:27-38.
  7. Amini, M., Younesi,  H., Bahramifar,  N., Lorestani,  A. A., Ghorbani,  F., Daneshi  A. and Sharifzadeh, M.(2008)Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger. J. Haz. Mat.  154:694-702.
  8. Soylak, M., Mendil, M. T. D. and Turkekul, I. (2006) Biosorption of heavy metals on Aspergillus fumigatus immobilized Diaion HP-2MG resin for their atomic absorption spectrometric determinations. Talanta. 70:1129-1135.
  9. Cochrane, E. L., Lu, S. and Gibb, S. W. A. (2006) comparison of low cost biosorbents and commercial biosorbents for the removal of copper from aqueous media. J. Haz. Mat. 137:198-206.
  10. Goksungur, Y., Uren, S. and Guvenc, U. (2005) Biosorption of cadmium and lead ions by ethanol treated waste baker's yeast biomass. Biores. Technol. 96:103-09.
  11. Aksu, Z., Calik, A., Dursun, A. Y. and  Derircan, Z. (1999) Biosorption of iron(III)-cyanide complex anions to Rhizopus arrhizus:application of adsorption isotherm. Proc. Biochem. 34:483-491.
  12. Ayer, A., Mody, K. and Bhavanath, J. (2005) Biosorption of heavy metal by a marine bacterium. Marine Pollution Bulletin 50:340-343.
  13. Chen, X. C., Wang, Y. P., Lin, Q. and Shi, J. Y. (2005) Biosorption of copper (II) and Zinc(II) from aqueous solution  by Pseudomonas putida CZ. Colloi. Surf. Biointerfac. 46:101-107.
  14. Loukidou, M. X., Matris, K. A. and Zouboulis, A. I. (2003) Removal of As (V)  from waste waters by chemically modified fungal biomass. Wat. Res. 37:4544-4552.
  15. Dursun, A. Y. (2006) A comparative study on determination of the equilibrium, kinetic and thermodynamic parameters of biosorption of copper (II) and lead (II) ions on to pretreated Aspergillus niger. Biochem. Eng. J. 28:187-195.
  16. Kratochvil, D. and Volesky, B. (1998)Advances in biosorption of heavy metals. Trends Biotech. 16:291-300.
  17. Zumriye, A. (2005) Application of biosorption for removal of organic pollutants: a review. Proc. Biochem. 40:997-1026.
  18. Viraraghavan, F. Y. T. (2001) Fungal decolourization of dye wastewaters: a review. Biores. Technol. 79:251-62.
  19. Sumathi, S. and Manju, B. (2000) Uptake of reactive textile dyes by Aspergillus foetidus. Enz. Microb. Technol. 27:347-52.
  20. Goksungur, Y., Uren, S. and Guvenc, U. (2005) Biosorption of cadmium and lead ions by ethanol treated waste baker's yeast biomass.  Biores. Technol. 96:103-109.
  21. Yalvac Can, M., Kaya, Y., Algur, O.F. (2006) Response surface optimization of the removal of nickel from aqueous solution by cone biomass of Pinus sylvestris. Biores. Technol. 97:1761-1765.
  22. Zümriye, A. (2005) Application of biosorption for the removal of organic pollutants: a review. Proc. Biochem. 40:997–1026.
  23. Tuzen, M., Uluozlu, O., Usta, C. and Soylak, M. (2007) Biosorption of copper(II), lead(II), iron(III) and cobalt(II) on Bacillus sphaericus-loaded Diaion SP-850 resin. Analytica Chimica Acta 581:241–246.
  24. Ahmet,C., Tamer, A., Sibel, T. and Serap, G. (2007) Biosorption of Pb(II) by industrial strain of Saccharomyces cerevisiae immobilized on the iomatrix of cone biomass of Pinus nigra:Equilibrium and mechanism analysis. Chem. Eng. J. 131:293–300.
  25. Guiqiu, C., Guangming, Z. and Lin, T. (2008)Cadmium removal from simulated waste water to biomass byproduct of Lentinus edodes. Biores. Technol.  99:7034-7040.

© 2008 Nigerian Society for Experimental Biology


The following images related to this document are available:

Photo images

[bk08007f5.jpg] [bk08007f4.jpg] [bk08007f2.jpg] [bk08007f7.jpg] [bk08007f6.jpg] [bk08007f1.jpg] [bk08007f3.jpg]
Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 05-Dec-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil