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The Journal of Food Technology in Africa, Vol. 6, No. 2, Apr-June, 2001, 59-62 Brine treatment, smoking and storage techniques: their effects on the microbial quality of smoked mackerel.
*Eyabi Eyabi G. D, **Hanson S. W. and **Barlow P. J. *Research Station for Fisheries and Marine Science of the Institute of Agriculture
Research for Development (IRAD), PMB 77 Limbe, Cameroon Code Number: ft01017
Abstract
Sample of Atlantic Mackerel (Scomber scombrus) were treated with 800 brine for 2 hours with slight agitation while untreated fish served as control. Both treated and untreated fish were loaded in a Torry Afos Mini Kiln set at 50° C. The temperature was raised to 80° C after one hour and maintained at this temperature throughout the smoking process.
Samples of treated and untreated fish (U8 and B8) were removed after 8 hours of smoking and the last batch removed at the end of 16 hours smoking (U16 and B16). Analyses for growth, identification and coverage of fish surface with mould and for sensory attributes of discoloration, off odours, brittleness (texture) and personal preference were carried out for freshly smoked and stored samples. Samples for storage were packaged in clear polythene and stored in an environmental cabinet.
Bringing and smoking time retarded mould growth, controlled discoloration, off odour development, softening of the fish and positively affected personal preference.
Penicillium sp was identified as the incriminated species. Keywords: Hot smoking, traditional smoking, water activity, brine strength, number of fish with mould growth and mean percentage coverage. Introduction
Traditional fish smoking (hot smoking) + is an important, one reason being that over 70% of artisanal fish landings in Cameroon (artisanal fishing contributes an estimated 75% of total national production) are converted to smoked/dried productions. In addition, smoked fish occupies an important place in the culinary habits of Cameroonians, contributing nearly 45% of animal proteins (Robinson and Crispoli, 1984).
Regrettably, traditional smoked fish are generally of variable quality and sometimes of poor quality, essentially manifested by mould growth a few days on storage. Mould growth affects not only acceptability, but also chemical and nutritional quality. There is the need to identify parameters of acceptability for smoked fish, to develop techniques for controlling and analysing mould growth and to establish the link between acceptable levels of preservatives and the levels that will give the required protection.
There is also need to establish the relationship between raw fish quality, smoke process technology, packaging and storage conditions and the microbial quality of hot smoked fish.
Some work has been carried out on the effects of treatment with salt and other preservatives (vinegar, BHT, BHA, citric acid, potassium sorbate etc.) smoking and the use of packaging on the quality of smoked fish, notably that recorded by Eyabi Eyabi and Ningo (1996), Eyabi Eyabi (1988), FAO (1981) and Bostock, Walker and Wood (1987). Salting and smoking after affect microbial growth through their effects on water activity (AW) ++, the high temperatures of hot smoking and the antimicrobial properties of wood smoke. These effects also have been noted in traditionally smoked long neck croaker (pseudotolithus typus) by Eyabi Eyabi (1993). Also, Zakhia, Coulibaly, Coulibaly and Cisse (1998) noted that vinegar and vinegar plus salt mixture delayed mould appearance on dried fish and this led to increased shelflife from 2 to 6 months.
The effects of salting and smoking time on sensory qualities partly relates to the moisture content/water actuity. For freshly smoked fish, salting and smoking time are most likely to influence flavour, texture and personal preference to mention, while on storage, they are discernible by measurements or perceptions of flavour, texture, colour and personal preference. These observations have been recorded by Eyabi Eyabi, 1988. Objectives The objectives of this study are to evaluate the effects of brining and smoking time on mould growth and organoleptic quality of freshly smoked and stored fish. Materials and Methods
Methods
One hundred and thirty two (132) fish collected from the chill store were gutted through the back, washed thoroughly to remove blood, dirt, entrails including hepatopancrease, gills, heart, liver and gut before dividing into 4 treatment groups as follows:
Thirty fish to be smoked for 8 hours (U8), 30 fish to be brined and smoked for 8 hours (B8), 30 fish to be smoked for 16 hours (U16) and 30 fish to be brined and smoked for 16 hours (B16) while the remaining 12 fish were frozen at -60° C to be used as control for chemical and nutritional analysis reported in another paper (Eyabi Eyabi, Hanson and Barlow, 2001).
Sixty fish (U8 and B8) were kept in a chill store, while 60 fish (B8 and B16) were brined in a salt solution of 80o +++ for 2 hours with limited agitation now and again.
After brining, both brined and unbrined samples were placed in a Torry Afos Mini Kiln set at an initial temperature at 50° C. After one hour, the temperature was raised to 80° C and maintained at this level throughout the rest of the "hot smoking process". Thirty unbrined (U8) and 30 - brined (B8) fish were removed from the oven after 8 hours of smoking and allowed to cool. The last batch of 30 unbrined (U16) and 30-brined (B16) were removed after 16 hours smoking.
On cooling to room temperature, 24 fish were randomly selected from the 30 fish of each treatment group on basis of wholesomeness and the balance of 6 fish were frozen stored at -60° C as a safeguard. The 24 fish from each treatment group were divided into 6 fish for each of the storage intervals (0, 4, and 12 weeks). The 6 fish were further divided into two groups of 3 fish each. The first group was used for chemical analysis reported in another paper (Eyabi Eyabi, Hanson and Barlow 2000) and for organoleptic quality while the second group was used to measure mould growth. Samples for storage were wrapped in clear polythene and stored in an environmental cabinet set at 30 (+2° C) and 75% to 85% Relative Humidity throughout the storage period. The number of fish with visible mould growth and mean percentage coverage was measured for the same 12 samples throughout the storage period (0, 2,4, 6,8, 19 and 12 weeks).
Organoleptic analyses were determined for attributes of discoloration, brittleness, off-odours and personal preferences on a 5 point hedonic scale by a panel of 12 judges for freshly smoked and stored samples (0, 4, 8, and 12 weeks).
In order to identify moulds growing on stored samples, mouldy growth on fish surface was scrapped off with sterile (wire) loop and inoculated on several slopes of sabourand and agar supplemented with chloramphenicol (0.05 mg/ml). Cultures were inoculated at 28° C and examined for gross and microscopic morphology. Microscopic morphology was studied on slide culture. Species identification of fungal isolates was made by comparison with standard description by Raper and Flannel (1965) and Raper and Thorn (1949). Statistical analysis
Organoleptic analyses and mould growths were measured on 12 samples (3 fish for each treatment). In order to determine treatment differences due to brining and smoking time, Analysis of Variance and Duncans Multiple Range Test (DMRT) were applied. Results and discussions
Figures I and II showed mould growth occurring in smoked fish during storage. It was evident from statistical analysis that both brining and smoking for 16 hours significantly delayed the onset of mould infection and the rate of spread of mould over the fish surface (P< 0.50; x= 68%, 24%, 43% and 4.6 for U8, B8, U16 and B16 respectively. These results are similar to those of and are in accordance with the recommendations of Eyabi Eyabi et. at. (1986), Eyabi Eyabi (1988), Lawal et. al. (1986) and FAO (1981) relating to the effects of salting and smoking in reducing incipient spoilage (mould growth etc.) and those of Zakhia et.al. (1981) relating to the effect that salting and dehydration (drying etc) delayed the onset of mould on dried fish leading to the extension of shelf-life from 2 to 6 months. These phenomena can be explained by a reduction of moisture content / water activity. (Table 1). Through salt binding with water, dehydration due to the application of heat especially during hot smoking (traditional smoking (50-1800C) (Eyabi Eyabi 1998). The pasteurizing / sterilizing effects of hot/traditional smoking and the antimicrobial agents present in wood smoke. In line with the above, sample U8 with an initial Aw of 0.95, 50% of the fish were infected after about 8 days and for samples B8 with an initial Aw of 0.81 (Table 1), 50% of the fish were infected after about 40 days. Investigation of the mould, indicated the prevalence of Penicillium sp.
Discolouration scores (Fig.III) tended to reflect growth and consequently water activity. Brining and to a lesser extent smoking time significantly retarded discolouration (P<0.050; x=3.9, 2.9, 3.4 and 2.2 for U8, B8, U16 and B16 respectively. These effects were most visible between 4 and 12 week's storage with a climax at the end of 8 weeks storage. The steady increase in discolouration scores for treatments B8 and B16 between 8 and 12 weeks corresponded to the period where there was a corresponding steady increase of mould coverage especially for B16 (Fig. I and II).
Brittleness (Fig. IV) was significantly influenced by both prolonged smoking (greater degree of dehydration) and brining (P< 0.05, x= 1.7, 2., 3.1, 3.7 for U8 B8, U16 and B16 respectively).
Off-odours (Fig. V). Measurement indicated that brining and prolonged smoking significantly slowed down, its development (P<0.050, x=3.5, 2.3, 2.6 and 1.8 for U8, B8, U16 and B16 respectively). The results are most indicative between 8 and 12 weeks storage. The most critical period in the development of off-odours was between 0 to 4 weeks for unbrined samples. Samples B8 showed a steady increase throughout storage while B16 virtually remained unchanged.
Personal preference (Fig. VI) Showed preference for treatment U8and B8 on freshly smoked samples. During storage, personal preference tended to decrease (5-1) as the discolouration and off-odour developed. The crucial factor determining personal preference appeared to be the growth of mould either directly through discoloration or indirectly through the development of off-odours. The latter was true of samples with shorter smoke treatment (U8, B8 (Fig. V and VI). The samples with longer smoke treatment had probably more of the smoke flavour that partially obscured off-odour. conclusions and recommendations
Penicillum spp. was the prevalent species recorded on stored mackerel. Brining and prolonged smoking inhibited mould growth possibly through their effects on water activity and also the presence of antimicrobial agents in wood smoke. The sensory measurements reflected mould growth with off-odours, personal preference on the decrease.
Whilst the level of salt and smoking time are being established in order to provide safe and stable products, it would be necessary on the one hand to consider acceptability, costs and health risks associated with the use of the salt and on the other, the use of food grade preservations to compliment or completely replace salt. References
Appendices
APPENDIX 1: Hot smoking - process in which fish is exposed to temperature
40 -800 C at some
APPENDIX 2: Water activity - describes the amount of water in a food available for microbial growth, enzymic and non- enzymic reactions. It can be measured as P/Po + N2/ (N1 + N2), Where P and Po are vapour pressure of solution and solvent and N1 + N2 are the number of solute and solvent respectively.
APPENDIX 3: 80 brine strength - The amount of salt in a solution measured by density, in brineometer degree where 10 = 26 4g salt/liter of water at 16° C and 80 = (26.4 x 8) = 211.2gm salt/litre of water at the same temperature (16 oC ).
APPENDIX 4: (1) Number of fish with visible mould growth represented by the formular ni / n x 100 where ni = number of fish in the treatment with evidence of mould attacked/growth and n=total number of fish in the treatment.
Mean percentage coverage represented by the formual SMij/n where M= Surface covered by mould, j= the number of replicates infested with mould and n= the number of replicates in the treatment.
APPENDIX 5: Discolouration (1 for no discoloration to 5 for extreme discolouration), off-odors ( 1 for no off-odour to 5 for extreme off-odour), brittleness (1 for not brittle to 5 for extremely brittle) and personal preference (1 for bad to 5 for excellent). Copyright 2001 The Journal of Food Technology in Africa, Nairobi The following images related to this document are available:Photo images[ft01017f4.jpg] [ft01017f1.jpg] [ft01017f6.jpg] [ft01017f3.jpg] [ft01017f2.jpg] [ft01017t1.jpg] [ft01017f5.jpg] |
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