|
Journal of Applied Sciences and Environmental Management
World Bank assisted National Agricultural Research Project (NARP) - University of Port Harcourt
ISSN: 1119-8362
Vol. 8, Num. 1, 2004, pp. 67-69
|
Journal of Applied Sciences & Environmental Management,
Vol. 8, No. 1, June, 2004, pp. 67-69
A Survey of Antibiotic
Resistant Staphylococcus
Aureus Strains from Clinical Sources
in Owerri
1*UWAEZUOKE, J C;
2ARIRIATU, L E
1Department of Microbiology, lmo State University,
Owerri
2Department of Biological Sciences, Federal University of Technology,
Owerri
*Corresponding author E-mail:
Code Number: ja04014
ABSTRACT:
A survey of antibiotic resistant
Staphylococcus aureus
strains from clinical specimens was carried out. A total of 100 different clinical
specimens were investigated with a yield of 48 Staphylococcus aureus
isolates. A high resistance of 95.8% to penicillin, 89.6% to ampicillin, 87.5%
to tetracycline, and 75.0% to chloramphenicol by Staphylococcus aureus strains
were recorded. A high susceptibility of 91.7% to gentamicin and 85.4% to cloxacillin
were also record. The high percentage resistance to the antibiotics studied
could be attributed to their prevailing usage and abuse in the area under
study. The implication of the high percentage recorded for the antibiotics
is that Staphylococcus aureus infections could be effectively treated with
gentamicin
and cloxacillin and not with penicillin, ampicillin, tetracycline, and chloramphenicol
in the area under study.@ JASEM
Drug resistance in microorganisms
is a predictable and perhaps inescapable response to the use of antimicrobial
agent. It can arise from the selection of resistant strains among naturally
susceptible species or from the ingress of new strains of naturally resistant
species. The extent of use of particular agents in a given environment dictates
the rate at which resistance arises
among microbial populations (Kunin, et al,1990). Some organisms rapidly
acquire resistance e.g. coliforms and Staphylococcus aureus, while others
rarely do so e.g. Streptococcus pyogenes (Sleigh and Timbury, 1986). The
emergence of drug-resistant bacteria is a major problem in
antibiotic therapy.
Staphylococcus
aureus has been recognized historically as a virulent and important human
pathogen. Its capacity to produce human disease has not diminished with the
introduction of antibiotics (Waldvogel, 1990). It is still one of the most frequently
encountered single bacterial species in hospitals and continues to be a frequent
cause of burns and wound sepsis (Emmerson, 1994). It produces pustules, carbuncles,
boils, and impetigo; it is also a frequent cause of septicaemia, osteomyelitis,
bacteraemia, and otitis (Emmerson, 1994; Shaposhnikova, et
al, 1995). Staphylococcus aureus is a very common cause of infection
in hospitals and is most liable to infect new born babies, surgical patients,
old and malnourished persons and patients with diabetes and other chronic diseases
(Tuo, et al, 1995).
Development
of resistance to antimicrbial agents by staphylococci is a major concern
primarily because they
are still frequently associated with hospital and
community acquired infections (Locksley, et al, 1982). The organisms
exhibit remarkable versatility in their behaviour towards antibiotics (Grassi,
1988), with some strains having overcome most commonly used drugs. Exposure
to new antibiotics often results in further selection of homologous resistant
strains (Haley, et al, 1982), a phenomenon particularly favoured by irrational
antibiotic administration. Infection with such resistant strains is likely to
be more severe and require longer hospitalization with incumbent increased costs,
than infection with susceptible strains (Baron, 1992). The present study was
designed to investigate the incidence of antibiotic resistant Staphylococcus
aureus strains from clinical sources in Owerri.
MATERIALS
AND METHODS
Forty-eight
strains of Staphylococcus aureus were isolated from clinical sources. The
specimens were obtained from different patients under medical attention in specialist
and private hospitals in Owerri, lmo State, Nigeria. The specimens obtained
were wound swabs, nasal swabs, high vaginal swabs and urine samples. The specimens
were collected as described by Cheesbrough (1984).
The samples were streaked
on mannitol salt agar (MSA) and blood agar (BA) plates. The plates were all
incubated at 37oC for 24 hours, after which the cultural and morphological
characteristics of the isolates were studied. Identification of isolates was
by standard microbiological methods as described by Cheesbrough (1984) and
Cowan (1993).
The antimicrobial sensitivity testing was carried out by
disc agar diffusion technique (Rapheal, et al, 1983) using Mueller-Hinton agar
plates. The discs used contained the following antibiotics: Ampicillin
(10mcg), Chloramphenicol (30 mcg), Cloxacillin (mcg), erythromycin (10 mcg), gentamicin
(10mcg), penicillin (10U), tetracycline (30mcg), streptomycin (10mcg).
RESULTS
AND DISCUSSION
100 clinical specimens were investigated
for the presence of Staphylococcus aureus (Table 1). The frequency
of isolation of Staphylococcus aureus from the different specimens analyzed
is given in table 1; a total of 48 isolates of Staphylococcus aureus were isolated.
The isolates were most sensitive to gentamicin (91.7%), cloxacillin (85.4%)
and most resistant to penicillin (95.8%) and ampicillin (89.6%), (Table 2).
The capacity of Staphylococcus aureus to produce human diseases has
not diminished with the introduction of
antibiotics (Waldvogel, 1990). The organisms exhibit remarkable versatility
in their behaviour towards antibiotics (Grassi, 1988), with some strains having
overcome most commonly used drugs. In this study, a high sensitivity percentage
to gentamicin (91.7%), Cloxacillin (85.4%) was recorded. Also most of the strains
of Staphylococcus aureus were sensitive to erythromycin (66.7%) and streptomycin
(66.7%). This finding shows that staphylococcal infections could be treated
with gentamicin, cloxacillin, erythromycin and streptomycin and corroborates
that of Oyagade and Oguntoyinbo (1997) and Uba
and Umar (2002). A percentage sensitivity of 4.2%, 10.4%, 12.5% and 25% were
recorded against penicillin, ampicillin, tetracycline and chloramphenicol respectively
in this study. Oyagade and Oguntoyinbo (1997) found a 2%, 22%, 32% and 58% sensitivity
to penicillin, ampicillin, tetracycline and chloramphenicol respectively which
were also relatively low (although higher than our
findings). Iroegbu, et al, (1997) in their study showed a sensitivity
of 43.3%, 58.6%. 41.4% and 30.9% to penicillin, ampicillin, tetracycline and
chloramphenicol
respectively by their Staphylococcus aureus strains. The variation found
in the sensitivity pattern to these commonly used drugs could be attributed to
the prevailing usage and abuse of the drugs in the areas under
study. The high rate of resistance to these commonly used drugs contrast with
the high sensitivity to gentamicin and cloxacillin, which are less frequently
used. This further suggests a relationship between antibiotic usage and the
level of drug resistance encountered in this study. The judicious use of antibiotics
by health workers and efforts to control procurement and use of antibiotics officially
in the locality will probably help to limit the increasing rate of drug resistance
in pathogens. It is the recommendation of this study that constant evaluation
of the antibiotic sensitivity pattern of pathogens for commonly used antimicrobial
agents in a particular environment be
carried out.
Table
1. Frequency of Staphylococcus aureus from different clinical specimens
Specimen
|
Number examined
|
Number isolated (% occurrence)
|
Wound
|
16
|
5 (10.4)
|
Nasal
|
29
|
16(33.3)
|
High vaginal
|
44
|
20 (41.7)
|
Urine
|
11
|
7(14.6)
|
Total
|
100
|
48
|
Table 2: Sensitivity pattern of Staphylococcus aureus from clinical
specimens
Isolates
|
No. of isolates
|
PN
|
CHI
|
CLX
|
E
|
GM
|
PEN
|
TE
|
Staph. aureus
|
48
|
5(10.4)
|
12(25)
|
41(85.4)
|
32(66.7)
|
44(91.7)
|
2(4.2)
|
6(12.5)
|
Key: Ampicillin =
PN, Chloramphenicol = CHL, Cloxacillin = CLX, Erythromycin = E, Gentamicin =
GM, Penicillin = PEN, Tetracycline = TE, Streptomycin = S.
REFERENCE
-
Baron,
E J. (1992). The detection, significance and rationale for control of methicillin resistant Staphylococcus
aureus. Clinical Microbiology News letter. 14:129.
-
Cheesbrough,
M. (1984). Medical Laboratory Manual for Tropical Countries. Vol 2: Microbiology.
Tropical Health Technology/Butter- worth and Co. Ltd. Cambridgeshire/Kent.
-
Cowan,
S T. (1993). Cowan and Steels manual for the identification of medical
bacteria. Cambridge University Press London.
-
Emmerson,
M. (1994). Nosocomial staphylococcal outbreak. Scandinavian Journal of lnfectious Diseases.
Suppl. 93:47-54.
-
Grassi,
G G. (1988). Infections by Gram positive bacteria: an overview. Journal
of antimicrobials and Chemotheraphy. 21 (Suppl. C): 1-7.
-
Haley, R N, Hightower, A
W, Khabbaz, R F, Thornsberry, C, Martone, W J, Allen, J R, Hughes,
J M. (1982). The emergence of methicillin resistant Staphylococcus aureus infections
in United State hospitals: possible role of the house-staff-patient transfer
circuit. Annals of internal Medicine 97: 297 308.
-
Iroegbu, C U, Ejimofor, O
D, Okpala, C N, Otti, N, Owuna, R. (1997) Staphylococcus aureus surveillance
in Nsukka, Nigeria: II. Antibiotic sensitivity pattern of
nasal isolates. Nigerian Journal of Microbiology. 11:15-19.
-
Kunin, C M, Johansen, K S,
Worning, A M, Daschner, F O. (1990). Report of a symposium on use
and abuse of antibiotics worldwide. Review of Infectious Diseases. 12:12 19.
-
Locksley,
R M, Cohen, M I, Quinn, T C, Tompkins, L S, Coyle, M B, Kirihara, J M,
Counts.(1982). Multiple antibiotic resistant Staphylococcus aureus: Introduction, transmission
and evolution of nosocomial infection. Annals
of internal medicine 9:317 324.
-
Oyagade, J O, and Oguntoyinbo,
F A. (1997). Incidence of antibiotic resistant Staphylococcus
aureus strains among isolates from environmental and clinical sources. Nigerian
Journal of Microbiology, 11 : 20 24.
-
Shaposhnikova, I. U G, Arutcheva,
A A, Okropiridze, G G, (1995) Nosocomial infections in presentdaytraumatological
or thopaedic hospitals. Vestn. Ross. Akad. Med. Nauk. 6 : 42 45.
-
Sleigh,
J D, and Timbury, M C. (1986). Notes on Medical Bacteriology. 2nd edn.
Churchill livingstone. Edinburgh. p 337
-
Tuo,
P, Montobbio, G, Vallarino, R, Tumolo, M, Calero, M G, Massone, M A. (1995). Nosocomial
staphylococci in a neonatal and pediatric intensive care unit. Pediatric Med.
Chir. 17 (2) : 117 122.
-
Uba,
A, and Umar, U. (2002) Incidence and the antibiotic susceptibility pattern
of Staphylococcus species
from clinical specimens in Bauchi, Nigeria. Book
of Abstracts, 26th Annual Conference of Nigerian Society for Microbiology,
University of Uyo, Akwa lbom State, Nigeria.
-
Waldvogel,
F A. (1990). Staphylococcus aureus (including toxic shock syndrome). In
: principles and practice of infectious disease. Mandell, G L, Douglas
R G Jr. and Benneth, J E. (eds). 3rd edn. Churchill Livingstone,
New York. pp
1489 1510.
Copyright 2004 - Journal of Applied Sciences & Environmental
Management
|