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Indian Journal of Medical Microbiology, Vol. 25, No. 2, April-June, 2007, pp. 177-178 Correspondence Circulating phage type of Vibrio cholerae in Mumbai SD Turbadkar, DP Ghadge, S Patil, AS Chowdhary, R Bharadwaj Department of Microbiology, Grant Medical College, Sir JJ Group of Hospitals, Mumbai - 4000 008, Maharashtra Correspondence Address:Department of Microbiology, Grant Medical College (SDT, ASC, RB), Grant Medical College, Sir JJ Group of Hospitals, Mumbai - 4000 008, Maharashtra, India; Department of Microbiology (SP), Government Medical College and Department of Microbiology (DPG), BJ Medical College, Sassoon General Hospital, Pune - 411 001, Maharashtra, India (email: sarojdt@yahoo.co.in) Date of Submission: 19-Aug-2006 Code Number: mb07056 Dear Editor, Cholera is endemic in several states of India. Bacteriophage typing is widely accepted as a convenient and highly discriminatory method of identifying epidemic strains of Vibrio cholerae . It is important to monitor the prevalent phage types within an area as introduction of new phage type may herald the onset of an outbreak. A total of 200 faecal samples, received between January and December 2004 from patients with acute diarrhoea, were screened for the presence of V. cholerae by standard laboratory methods, [1] and identified by routine biochemical tests and confirmed by slide agglutination with antisera obtained from National Institute of Cholera and Enteric Diseases, Kolkata. Antibiotic susceptibility test was done by Kirby Bauer disc diffusion method. [2] The strains were then sent for phage typing to National Institute of Cholera and Enteric Diseases, Kolkatta. A total of 41 (20.5%) V.cholerae were isolated. All the strains were biotype El Tor, serotype Ogawa and belonged to phage type 4. These strains were typable with new scheme and were clustered in to type 27 (97.5%) and type 23 (2.4%) respectively. Vibrio cholerae isolates showed maximum sensitivity in vitro to gentamicin (92.6%) and chloramphenicol (87.8%) respectively [Table - 1]. In spite of the complexities of the environment and a large migratory population in Mumbai, all the isolates belonged to Basu and Mukerjee phage type 4. Type 2 and 4 are the prevalent biotypes in India at present [3] but consistently getting only a single biotype over the year was surprising. However, the new scheme was more discriminatory and could identify two circulating types. Even then the majority belonged to types 27 (97.5 %) and only 2.4 % were type 23. These were found in the month of August and were likely to be imported phage type into the city. Though prevalence of multi-drug resistant Vibrio cholerae has been reported from parts of India, [4],[5] we did not encounter any such strains. A decrease in sensitivity to ampicillin and tetracycline was observed. References
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