 |
| About Bioline | All Journals | Testimonials | Membership | News |
|
||||||
|
||||||
Indian Journal of Medical Microbiology, Vol. 28, No. 1, January-March, 2010, pp. 80-81 Correspondence Emergence of optochin resistance among S. pneumoniae strains colonizing healthy children in Abidjan *A Kacou-N’douba, SC Okpo, E Ekaza, A Pakora, S Koffi, M Dosso Department of Bacteriology, Pasteur Institute of Cote d’Ivoire (KNA,OCS,EE,PA,DM), Virology-01 BP 490, Department of Microbiology, Unit of Formation and Research of Medical Sciences Abidjan (KNA, KS), University of Cocody 01 BP V 166, University Hospital Center of Cocody (KNA,KS), Clinical Bacteriology Unit - 01 BP V 33 Abidjan 01, Cote d’Ivoire. Correspondence Address: *Department of Bacteriology, Pasteur Institute of Cote d'Ivoire, Virology-01 BP 490 BP V 33 Abidjan 01, Cote d'Ivoire, knadele@yahoo.fr Date of Submission: 14-Apr-2009 Code Number: mb10025 PMID: 20061777 DOI: 10.4103/0255-0857.58742 Dear Editor, Streptococcus pneumoniae is one of the most important infection pathogens in the world, particularly in children below five years. This bacteria nasopharyngeal carriage is an important risk factor in the occurrence of pneumococcal diseases. [1] Identification of S. pneumoniae has conventionally been based on phenotypic characteristics. However, optochin-resistant pneumococcal isolates have been reported in the world. This study aims at determining the prevalence of optochin-resistant pneumococcal isolates in children carriers in Abidjan. A total of 266 strains were isolated in 966 under-five healthy children. Both Isolation and identification of the infection pathogens were based on selective growth of gentamicin blood agar plates, colonies morphological aspect, optochin sensibility, bile solubility, agglutination test and spotting of pneumolysin gene. [2] The optochin sensibility test was performed using six mm diameter optochin disc containing five μg (oxoid, Hampshire, England) within an atmosphere of five per cent CO 2 . Isolates were considered resistant when the inhibition diameter was under 14 mm. Antimicrobial susceptibility was realised using Kirby-Bauer disk diffusion method according CAS-FM 2006 guidelines. When the oxacilin (5 μg) inhibition diameter was less than 26 mm, determination of penicillin minimum inhibitory concentration (MICs) was done using E-test (AB Biodisk, Solna, Sweden) according to CLSI 2009 guidelines. S. pneumoniae (ATCC 49619) was used as control strain. Serotyping was performed using Quellum reaction with antisera produced by the Statens Serum Institute (Copenhagen, Denmark). [3] Three (1.1%) of the 266 isolates of S. pneumoniae from carriers were found to be optochin-resistant. These strains belonged to three different serotypes - 18, 19 and 23 [Table - 1]. They were also multidrug-resistant. This optochin resistance may be due to mutations. [4] There may also be an association between the use of antimalarial drugs and evolution of optochin resistance. [5] Surveillance of optochin-resistant isolates should be continued to study different mutation types. References
Copyright 2010 - Indian Journal of Medical Microbiology The following images related to this document are available:Photo images[mb10025t1.jpg] |
| |||||||||
