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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 6, Num. 1, 2003, pp. 59-61
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African Journal of Biomedical Research, Vol. 6, No. 1, Jan, 2003, pp. 59-61
Short communication
PESTE DES PETITS RUMINANTS (PPR) VIRUS ANTIBODIES IN AFRICAN
GREY DUIKER ( SYLVICAPRA GRIMMIA )
A.O. OGUNSANMI 1 , E.O. AWE 4 , T.U. OBI 2 AND V.O. TAIWO
3 *
Departments of 1 Wildlife and Fisheries Management; 2 Veterinary
Medicine; 3 Veterinary Pathology, University of Ibadan . Ibadan
. Nigeria . 4 College of Medicine, Ladoke Akintola University
of Technology, Ogbomosho , Nigeria .
*Corresponding Author:
Received: May 2002
Accepted: January 2003
Code Number: md03011
ABSTRACT
The prevalence rate of antibodies to peste des petits ruminants virus (PPRV)
and rinderpest Virus (RPV) antigens was studied using 38 sera samples collected
from African grey duiker (Sylvicapra grimmia). Of the total, 4 (10.5%) were
positive for antibodies to PPRV, while none (0%) was positive for RPV. The
role of wildlife in the epizootiology of peste des petits ruminants is discussed.
Key words: African grey duiker, Sheep. Goats, PPRV, RPV, Antibodies.
INTRODUCTION
Peste des petits ruminants (PPR) is an acute, highly contagious viral disease
of sheep, goats and wild ruminants that is endemic in several countries in
Africa the Arabian Peninsula, Middle East and India (Taylor, 1984; Wamwayi et
al ., 1995; Shaila et al ., 1996; Govindarajan et al , 1997).
The virus is antigenically related to rinderpest virus which infects cattle
and other large ruminants (Barrett, 1994). It is a member of the Morbillivirus genus,
which also includes measles, canine distemper and viruses of marine mammals
in the family Paramyxoviridae (Barrett et al ., 1993). PPR is characterized
by fever, mucopurulent oculo-nasal discharge, diarrhoea, dehydration, ulceration
of the buccal cavity and pneumonia. Gross and microscopic lesions in the natural
and experimental disease have been described in domestic small ruminants (Ikede,
1983 Uzuokwu, 1983) and in wild white-tailed deer ( Odocoileus virginianus ).
PPR is considered to be one of the main constraints to improving productivity
of small ruminants in the regions where it is endemic (Ikede, 1983). It is
of great economic importance on the basis of mortalities, morbidity, losses
through body wastage, poor feed efficiency, loss of meat, milk and milk products
and offspring (Nawathe, 1984).
Programs aimed at controlling PPR in ruminants have cost farmers, governments
and aid agencies large sums of money throughout the last century. A comprehensive
quantification of the economic importance of PPR, however, has never been attempted,
mainly due to a paucity of reliable data on such important factors as the distribution
and numbers of animals at risk, insufficient knowledge of the ethics of the
disease on livestock production., and the difficulty in assessing the quantification
of risk factors on livestock and their products in pastoral and mixed crop/livestock
subsistence production systems. Furthermore the role of wildlife in the epizootiology
of PPR has not been fully elucidated. The isolation of virus from an outbreak
in Indian buffalo ( Bubalus bubalis ) has been reported (Govindarajan et
al , 1997) to occur at a District Livestock Farm in Orathanadu, Tanjore
District, Tamil Nadu where 50 of the 385 buffaloes were affected. The source
of infection in the buffalo herd could not be traced since there was no reported
incidence of PPR in small ruminants in the vicinity. However, the local animal
husbandry department has reported confirmed outbreaks of small ruminants PPR
in adjacent districts in the preceeding months (Govindarajan et al ,
1997). The occurrence of PPR in a form that is difficult to identify clinically
in buff therefore assumes epizootological significance.
Apart from vaccination using tissue culture rinderpest vaccine (TCRV), there
is no PPRV vaccine in Nigeria , and there is also no specific treatment for
PPR. However, several attempts have been made to alter the course of the disease
through the use of antibiotics, fluid replacement therapy and antidiarrhoeal
drugs (Mornet et al , 1956; Wosu, 1989; Ajala et al , 1997).
Recently, specific rinderpest virus (RV) and peste des petits ruminants virus
monoclonal antibody based competitive enzyme-linked immunosorbent assays (cELISA)
have been developed (Anderson et al, 1991). This has made it possible to rapidly
differentiate infections with PPRV as distinct from those with RPV. Thus, the
aim of the present study was to screen sera samples of grey duiker ( Sylvicapra
grimmia ) for PPRV and RPV using cELISA technique. This is facilitated
by rapidly emerging trend of increased grey duiker domestication and consumption
patterns in Nigeria (Ogunsanmi et al , 2001).
MATERIALS AND METHODS
Sample collection and assay for RPV and PPRV by cELISA
Sera samples from thirty-eight (38) African grey duiker ( Sylvicapra grimmia )
were collected from Irewole Local Government Area of Osun State in the rain
forest vegetation of Nigeria . Samples were examined for haemagglutinin (HI)
protein of RPV and PPRV using specific RPV and PPRV monoclonal antibody-based
competitive enzyme-linked immunosorbent assays (cELISA) developed by Anderson et
al . (1991). Briefly, microtitre immunoplates (NUNC, Denmark ) were coated
with 1:100 dilutions of the RPV and PPRV antigens in PBS (PH 7.4) and incubated
at 37 0 C for 1hr on an Orbital shaker (Luckham. Sussex , UK ). The working
volume was 50 µ/well.
Following three washings with PBS and drying the plates on paper towels, the
sera were added to all wells except the strong positive control, moderate positive,
negative control, monoclonal control and conjugate control wells. The plates
were covered and incubated on shaker at 37° C for 1 hr and washed three
times before the addition of 50 mI/well of rabbit anti-mouse IgG conjugate
with horseradish peroxidase (Sigma Chemical Company, USA ) in blocking buffer.
The plates were incubated as described above. Following further washing and
drying step, the chromophore/substrate i.e. orthophenylene diamine (OPD), hydrogen
peroxide mixture was added and the colour reaction stopped after 10mins with
1m sulphuric acid. The optical densities (OD) were then read on a spectrophotometer
(Multiscan Plus, MK 11, Flow Laboratories, UK ) at 492nm.
The percentage inhibition of a given MAb (monoclonal antibody) was calculated
from optical densities (OD) of the sample according to the following formula:
% inhibition = 100 x (OD of the sample/OD of the control)
The OD of the sample was the OD in the presence of inhibitor and OD of the
control was the OD without inhibitor. An inhibition of more than 50 percent
was considered positive.
RESULTS AND DISCUSSION
The results of the cELISA tests for PPRV and RPV antibodies in the sera of
gray duiker and presented in Table 1. A total of 38 sera from grey duiker were
examined in this study. Of the total 4 (10.5%) were positive for PPRV while
none (0.0%) was positive for RPV antibodies (Table 1). The detection of antibodies
against PPRV in the present study probably implicates the role of wildlife
in the epizeotiology of PPR in Nigeria . The results of this study also indicate
PPRV may have gained access to this region through wildlife, especially wild
small ruminants which are prominently hunted animals in this environment (Ogunsanmi et
al ., 2001).
Table 1: Peste des Petits Ruminants Virus (PPRV) and Rinderpest Virus (RPV) antibodies
in
the sera of grey duiker in Irewole Local Government Area (LGA) Osun State
, Nigeria
No. of samples |
cELlSA antibody test |
Number positive |
Percentage positive |
38 |
PPRV |
4 |
10.5 |
38 |
RPV |
0 |
0.0 |
The National Livestock Project Division (NLRD) of Nigeria PPR vaccination
campaign is being carried out in small ruminants in the adjoining Lagelu Local
Government Area of Oyo State in the preceding months. In a follow-up study
in Lagelu Local Government Area, Akpavie et al . (1997) reported that
69.4% and 85.4% of goats and sheep respectively, showed antibody to PPRV. Although
RPV and PPRV are diseases of ruminants, which have the same geographical distribution
in Africa , antibody to RPV was not detected in the examined grey duikers sera
in this study. The reason(s) for this could not be ascertained. The results
of this study suggest a need for continuous serological and clinical surveillance
of PPR in wild ruminants in order to determine the prevalence of PPR, its effects
on wildlife conservation and the possible role of these species in the transmission
cycle of PPRV.
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