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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 6, Num. 1, 2003, pp. 59-61

African Journal of Biomedical Research, Vol. 6, No. 1, Jan, 2003, pp. 59-61

Short communication

PESTE DES PETITS RUMINANTS (PPR) VIRUS ANTIBODIES IN AFRICAN GREY DUIKER ( SYLVICAPRA GRIMMIA )

A.O. OGUNSANMI 1 , E.O. AWE 4 , T.U. OBI 2 AND V.O. TAIWO 3 *

Departments of 1 Wildlife and Fisheries Management; 2 Veterinary Medicine; 3 Veterinary Pathology, University of Ibadan . Ibadan . Nigeria . 4 College of Medicine, Ladoke Akintola University of Technology, Ogbomosho , Nigeria .
*Corresponding Author:

Received: May 2002
Accepted: January 2003

Code Number: md03011

ABSTRACT  

The prevalence rate of antibodies to peste des petits ruminants virus (PPRV) and rinderpest Virus (RPV) antigens was studied using 38 sera samples collected from African grey duiker (Sylvicapra grimmia). Of the total, 4 (10.5%) were positive for antibodies to PPRV, while none (0%) was positive for RPV. The role of wildlife in the epizootiology of peste des petits ruminants is discussed.  

Key words: African grey duiker, Sheep. Goats, PPRV, RPV, Antibodies.

INTRODUCTION

Peste des petits ruminants (PPR) is an acute, highly contagious viral disease of sheep, goats and wild ruminants that is endemic in several countries in Africa the Arabian Peninsula, Middle East and India (Taylor, 1984; Wamwayi et al ., 1995; Shaila et al ., 1996; Govindarajan et al , 1997). The virus is antigenically related to rinderpest virus which infects cattle and other large ruminants (Barrett, 1994). It is a member of the Morbillivirus genus, which also includes measles, canine distemper and viruses of marine mammals in the family Paramyxoviridae (Barrett et al ., 1993). PPR is characterized by fever, mucopurulent oculo-nasal discharge, diarrhoea, dehydration, ulceration of the buccal cavity and pneumonia. Gross and microscopic lesions in the natural and experimental disease have been described in domestic small ruminants (Ikede, 1983 Uzuokwu, 1983) and in wild white-tailed deer ( Odocoileus virginianus ). PPR is considered to be one of the main constraints to improving productivity of small ruminants in the regions where it is endemic (Ikede, 1983). It is of great economic importance on the basis of mortalities, morbidity, losses through body wastage, poor feed efficiency, loss of meat, milk and milk products and offspring (Nawathe, 1984).

Programs aimed at controlling PPR in ruminants have cost farmers, governments and aid agencies large sums of money throughout the last century. A comprehensive quantification of the economic importance of PPR, however, has never been attempted, mainly due to a paucity of reliable data on such important factors as the distribution and numbers of animals at risk, insufficient knowledge of the ethics of the disease on livestock production., and the difficulty in assessing the quantification of risk factors on livestock and their products in pastoral and mixed crop/livestock subsistence production systems. Furthermore the role of wildlife in the epizootiology of PPR has not been fully elucidated. The isolation of virus from an outbreak in Indian buffalo ( Bubalus bubalis ) has been reported (Govindarajan et al , 1997) to occur at a District Livestock Farm in Orathanadu, Tanjore District, Tamil Nadu where 50 of the 385 buffaloes were affected. The source of infection in the buffalo herd could not be traced since there was no reported incidence of PPR in small ruminants in the vicinity. However, the local animal husbandry department has reported confirmed outbreaks of small ruminants PPR in adjacent districts in the preceeding months (Govindarajan et al , 1997). The occurrence of PPR in a form that is difficult to identify clinically in buff therefore assumes epizootological significance.

Apart from vaccination using tissue culture rinderpest vaccine (TCRV), there is no PPRV vaccine in Nigeria , and there is also no specific treatment for PPR. However, several attempts have been made to alter the course of the disease through the use of antibiotics, fluid replacement therapy and antidiarrhoeal drugs (Mornet et al , 1956; Wosu, 1989; Ajala et al , 1997). Recently, specific rinderpest virus (RV) and peste des petits ruminants virus monoclonal antibody based competitive enzyme-linked immunosorbent assays (cELISA) have been developed (Anderson et al, 1991). This has made it possible to rapidly differentiate infections with PPRV as distinct from those with RPV. Thus, the aim of the present study was to screen sera samples of grey duiker ( Sylvicapra grimmia ) for PPRV and RPV using cELISA technique. This is facilitated by rapidly emerging trend of increased grey duiker domestication and consumption patterns in Nigeria (Ogunsanmi et al , 2001).

MATERIALS AND METHODS

Sample collection and assay for RPV and PPRV by cELISA

Sera samples from thirty-eight (38) African grey duiker ( Sylvicapra grimmia ) were collected from Irewole Local Government Area of Osun State in the rain forest vegetation of Nigeria . Samples were examined for haemagglutinin (HI) protein of RPV and PPRV using specific RPV and PPRV monoclonal antibody-based competitive enzyme-linked immunosorbent assays (cELISA) developed by Anderson et al . (1991). Briefly, microtitre immunoplates (NUNC, Denmark ) were coated with 1:100 dilutions of the RPV and PPRV antigens in PBS (PH 7.4) and incubated at 37 0 C for 1hr on an Orbital shaker (Luckham. Sussex , UK ). The working volume was 50 µ/well.

Following three washings with PBS and drying the plates on paper towels, the sera were added to all wells except the strong positive control, moderate positive, negative control, monoclonal control and conjugate control wells. The plates were covered and incubated on shaker at 37° C for 1 hr and washed three times before the addition of 50 mI/well of rabbit anti-mouse IgG conjugate with horseradish peroxidase (Sigma Chemical Company, USA ) in blocking buffer. The plates were incubated as described above. Following further washing and drying step, the chromophore/substrate i.e. orthophenylene diamine (OPD), hydrogen peroxide mixture was added and the colour reaction stopped after 10mins with 1m sulphuric acid. The optical densities (OD) were then read on a spectrophotometer (Multiscan Plus, MK 11, Flow Laboratories, UK ) at 492nm.

The percentage inhibition of a given MAb (monoclonal antibody) was calculated from optical densities (OD) of the sample according to the following formula:

% inhibition = 100 x (OD of the sample/OD of the control)

The OD of the sample was the OD in the presence of inhibitor and OD of the control was the OD without inhibitor. An inhibition of more than 50 percent was considered positive.

RESULTS AND DISCUSSION

The results of the cELISA tests for PPRV and RPV antibodies in the sera of gray duiker and presented in Table 1. A total of 38 sera from grey duiker were examined in this study. Of the total 4 (10.5%) were positive for PPRV while none (0.0%) was positive for RPV antibodies (Table 1). The detection of antibodies against PPRV in the present study probably implicates the role of wildlife in the epizeotiology of PPR in Nigeria . The results of this study also indicate PPRV may have gained access to this region through wildlife, especially wild small ruminants which are prominently hunted animals in this environment (Ogunsanmi et al ., 2001).

Table 1: Peste des Petits Ruminants Virus (PPRV) and Rinderpest Virus (RPV) antibodies in
the sera of grey duiker in Irewole Local Government Area (LGA) Osun State , Nigeria

No. of samples

cELlSA antibody test

Number positive

Percentage positive

38

PPRV

4

10.5

38

RPV

0

0.0

The National Livestock Project Division (NLRD) of Nigeria PPR vaccination campaign is being carried out in small ruminants in the adjoining Lagelu Local Government Area of Oyo State in the preceding months. In a follow-up study in Lagelu Local Government Area, Akpavie et al . (1997) reported that 69.4% and 85.4% of goats and sheep respectively, showed antibody to PPRV. Although RPV and PPRV are diseases of ruminants, which have the same geographical distribution in Africa , antibody to RPV was not detected in the examined grey duikers sera in this study. The reason(s) for this could not be ascertained. The results of this study suggest a need for continuous serological and clinical surveillance of PPR in wild ruminants in order to determine the prevalence of PPR, its effects on wildlife conservation and the possible role of these species in the transmission cycle of PPRV.

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