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African Journal of Biomedical Research
Ibadan Biomedical Communications Group
ISSN: 1119-5096
Vol. 9, Num. 3, 2006, pp. 199-204
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African Journal of Biomedical Research, Vol. 9, No. 3, Sept, 2006, pp. 199-204
Full Length Research Article
Reproductive Tract
Morphometry and Some Haematological Characteristics of Female Rabbits Fed
Pawpaw Peel Meal Based Diets
I.I. Bitto*, J.A, Arubi and A.A.
Gumel
Department
of Animal Breeding and Physiology, University
of Agriculture, Makurdi, Makurdi-.
Nigeria
*Address for Correspondence
(e-mail Address): sibittos@yahoo.com
Received: December
2005
Accepted
(Revised): July
2006
Published: September,
2006
Code Number: md06032
ABSTRACT
16 female grower rabbits were
randomly assigned to any of 4 iso-nitrogeneous and iso-caloric diets containing
0%, 10%, 20% and 30% pawpaw meal (PPM) such that to each dietary treatment were
4 does. After 7 weeks of ad libitum feeding, all the animals were sacrificed
and evaluated for reproductive tract morphometry and some haematological
characteristics. The results showed similarities (P>0.05) between the diets
for all the parameters evaluated. The results suggest that dietary PPM up to
30% level of inclusion may support normal body functions and the physiology of
reproduction in female rabbits.
Keywords: Pawpaw
peels, haematology, reproductive tract, morphometry
INTRODUCTION
The
ability of rabbits to convert forage crop residues and agro-industrial
by-products more efficiently into meat than most other livestock (Aduku and
Olukosi, 1990, Fielding, 1991) has not been applied much beyond research in the
country. It is hoped that many of the unconventional feed stuff now being
tested in practical diets for rabbits will soon become popular with rabbit
farmers just like Tridax procumbens, a popular forage which is used in
many parts of the guinea savannah as a sole diet for rabbits. Tridax is however
seasonal in the guinea savannah as the practice of bush burning by hunters
destroys much of the vegetation during the dry season.
The
pawpaw plant (Carica Papaya Linn) which stands about 4-5m tall on the average
but could reach 10m in height always survives the bush fire as it is grow
mostly around homes. Parts of the pawpaw plant have been reported to be rich in
nutrients (Oyenuga, 1968; Aduku, 1988; Nakasone and Paul, 1998) and are
suitable for the feeding of rabbits especially, in the dry season. The latex in
all parts of the pawpaw plant however contains a high percentage of papain- a proteolytic
enzyme used in several industries. Papain however has been implicated in
reproductive disorders in both male and female animals (Gwatkin, 1964; Grag, et
al 1970; Egbunike et al, 2000) and shown to significantly decrease
the absolute and relative weights of the liver in rabbits (Bitto and Gemade,
2001). Feeding pawpaw parts therefore must be balanced with an assessment of
possible effects of residual papain in processed or fresh pawpaw parts on the
physiology of reproduction in the sexes. The current lack of information on
reproductive tract morphometry and the hematology of female rabbits fed pawpaw
parts therefore necessitated this work.
MATERIALS AND
METHODS
Location:
This study was conducted at a
standard Rabbitary (approved for research by the Department of Animal
Production, University of Agriculture Makurdi) at the Federal Housing Authority
Estate Makurdi, Nigeria. Makurdi is located at latitude 7°14N and
longitude 8°21E with an annual rain fall ranging from 1270-1397mm
and a temperature range of 21°c-42°c.
Animals
and Management: 16 grower rabbits of
mixed breeds (Chinchilla x California x Newzealand White) between the ages of
9and 11 weeks with a mean initial weight of 1,200g were used for this study.
They were housed in individual cages measuring 1.5m x 1m x1m with corrugated
roofing sheets and wire mesh floor with wooden frames. They were fed a maize
based concentrate diet for a week of acclimatization with cool clean drinking
water supplied always before the commencement of experimental feeding.
Pawpaw
Peels: Unripe pawpaw fruits were
obtained from Gboko, Otukpo and Makurdi towns in Benue State, Nigeria. The
peels were carefully removed form the pulp immediately after harvest and sun
dried for 7 consecutive rain free days and there after ground for incorporation
into the test diets as pawpaw peel meal (PPM).
Experimental
Diets: 4 isocaloric and
isonitrogenous diets were compounded with diet I (control) containing no PPM
while diets 2,3, and 4 contained 10%, 20% and 30% PPM respectively. A
completely randomized design was used to assign the animals to the experimental
diets such that there were 4 does on each diet. The animals were fed the diets ad
libitum with cool clean drinking water supplied always. The does were
weighed individually weekly. The proximate compositions of the experimental
diets were determined by the A.O.A.C (1990) method. The does were fed the test
diets for 7 weeks.
Sampling:
After 7 weeks of feeding, all the
animals were starved for 12 hours and thereafter sacrificed by stunning and
decapitation.
Haematological
Analysis: Blood samples were
collected at slaughter into clean dry test tubes containing a pinch of an
anticoagulant - Ethylene diamine tetra acetic acid (EDTA) for haemotological
analysis. All haematological parameters were evaluated as earlier fully
described by Bitto and Gemade (2001).
Reproductive
tract morphometry: Each animal was
dissected immediately after slaughter and the reproductive tract obtained intoto,
trimmed free of fat and adhering connective tissue before morphometric
analysis. The weight of each reproductive tract was taken intoto after
which each ovary was carefully removed from its ovarian bursa at the end of its
infudibulum. The infundibulum was next removed followed by the oviduct. The
uterine horn was then taken from the end of the two cervixes to the rosette
projection of the uterotubal junction. Morphometric evaluations were done
using highly sensitive digital balances while linear measurements were taken
with well-calibrated rules.
Statistical
Analysis: Data were subjected to the
one way analysis of variance (ANOVA) using the completely randomized design as
outlined by Steel and Torrie (1980).
RESULTS
AND DISCUSSION
The
gross and chemical compositions of the diets and PPM are shown in Tables 1 and
2 respectively, while the effects of PPM on reproductive tract morphometry are
summarized in Tables 3 and 4. PPM had no effect (P>0.05) on the morphometric
characteristics of the reproductive organs and their derivations from body
weight. Even though there is a lack of data in the literature on the
morphometric characteristics of the reproductive organs of female rabbits with
which our results could be compared, the non effect of diet on reproductive
tract morphometry obtained in this study suggests that the development of the
reproductive organs, reproductive processes and perhaps fertility may not be
affected when PPM is included in the diets of female rabbits up to a level of
30%. Being that uterine weight increase in rodents has been used as a
bioindicator of the presence of estrogens (Hafez, 1980), further work
establishing endogenous levels of estrogens when female rabbits are fed PPM is
required. Also, going by the parameters investigated in the present study, the implication
of papain in reproductive disorders in the mouse (Gwatkin, 1964) and in the rat
(Grag et al, 1970) are at variance with our results.
Table
1: Composition
of the Experimental Diets (%)
Ingredients |
1(0%) |
2(10%) |
3(20%) |
4(30%) |
Maize |
30.16 |
33.39 |
37.66 |
41.43 |
Soyabean
meal |
28.12 |
22.67 |
17.19 |
11.81 |
Rice
offals |
35.32 |
27.54 |
18.75 |
10.36 |
Pawpaw
peels |
- |
10 |
20 |
30 |
Vitamin
premix |
0.5 |
0.5 |
0.5 |
0.5 |
Palm
oil |
1 |
1 |
1 |
1 |
Bone
meal |
4 |
4 |
4 |
4 |
Methionine |
0.4 |
0.4 |
0.4 |
0.4 |
Salt |
0.5 |
0.5 |
0.5 |
0.5 |
Total |
100 |
100 |
100 |
100 |
Table
2: The
Chemical Composition of the Experimental Diets and Pawpaw peel meal (%)
Parameters
|
1(0%) |
2(10%) |
3(20%) |
4(30%) |
PPM** |
Dry
matter |
98.50
|
97.98 |
97.87 |
97.87
|
97.20 |
Crude
protein |
20.25 |
20.94 |
18.38 |
17.44 |
17.50 |
Crude
fibre |
19.40 |
17.69 |
15.18 |
12.94 |
13.30 |
Ether
extract |
11.20 |
12.67 |
10.47 |
11.71 |
8.75 |
Ash |
13.82 |
12.91 |
11.70 |
11.70 |
9.10 |
M.E
(Kcal/kg)* |
2850.46
|
2992.60
|
3012.40
|
3041.66
|
3073.00 |
= Calculated from
Pauzenga (1985); ** =Paw paw peel meal
Table
3: The
effect of pawpaw peel meal on female reproductive tract morphometry (mean ±
s.e.m)*
Parameters
|
1(0%)
|
2(10%)
|
3(20%)
|
4(30%)
|
Body
weight (g)
|
1383
±116.91
|
1600±94.39
|
1500±108.14
|
1550±23.60
|
Weight
of tract (g)
|
2.24
±0.0659
|
3.40±0.77
|
1.62±0.38
|
1.94±0.66
|
Paired
ovary weight (g)
|
0.229
±0.022-
|
0.294±0.036
|
0.234±0.016
|
0.119±0.043
|
Paired
infundibulum Weight (g)
|
0.12
±0.025
|
0.101±0.023
|
0.154±0.19
|
0.124±0.038
|
Paired
uterine horn weight (g)
|
0.64
±0.232
|
1.33±0.343
|
0.55±0.076
|
0.97±0.043
|
Cervix
weight (g)
|
0.49±0.152
|
0.58±0.213
|
0.25±0.042
|
1.097±0.409
|
Vagina
weight (g)
|
0.58±0.199
|
0.93±0.223
|
0.46±0.215
|
0.36±0.076
|
Paired
oviduct Weight (g)
|
0.122±0.37
|
0.196±0.023
|
0.109±0.031
|
0.183±0.074
|
Width
of uterine horn (cm)
|
0.933±0.054
|
1.067±0.152
|
0.933±0.027
|
0.90±0.180
|
Length
of cervixes
|
0.87±0.196
|
1.37±0.191
|
0.92±0.027
|
0.87±0.189
|
Length
of ovary (cm)
|
1.90±0.163
|
1.73±0.089
|
2.27±0.348
|
0.90±0.189
|
Length
of oviduct (cm)
|
6.00±0.794
|
9.20±0.601
|
8.73±0.04
|
|
Length
of uterine horn (cm)
|
8.07±0.409
|
6.40±2.130
|
10.83±0.34
|
0.87±160±0.191
|
sem = standard
error of mean: * = (P>0.05)
Table 4: Derivations from reproductive tract morphometry based
on body weight (mean± s.e.m)*
Parameters
|
1(0%)
|
2(10%)
|
3(20%)
|
4(30%) |
Vagina |
0.039±0.012 |
0.057±0.012 |
0.029±0.012 |
0.066±0.038 |
Cervixes
|
0.033±0.009 |
0.035±0.011 |
0.017±0.002 |
0.023±0.011 |
Paired
Infundibulum |
0.009±0.002 |
0.006±0.001 |
0.010±0.001 |
0.009±0.002 |
Paired
oviduct |
0.008±0.002 |
0.012±0.001
|
0.007±0.002 |
0.012±0.005 |
Paired
ovary |
0.017±0.003 |
0.019±0.003 |
0.015±0.002 |
0.008±0.003 |
Paired
uterine horn |
0.043±0.014 |
0.082±0.019 |
0.036±0.003 |
0.063±0.026 |
S.E.M =standard error of mean; * =
(P>0.05)
Table 5: The effect of pawpaw peel meal on the haematology of
grower female Rabbits (means ± sem)*
Parameters |
1(0%) |
2(10%) |
3(20%) |
4(30%) |
Hb
(g/dl) |
7.86±0.65 |
7.55±0.43 |
6.80±0.93 |
6.64±0.84 |
PCV(%) |
24±1.17 |
23±1.36 |
20±1.36 |
20±1.85 |
WBC
(X103/mm) |
3.13±0.05
|
3.97±0.09 |
3.1±0.07 |
3.56±0.79 |
MCV
(µ3/m) |
68.00±0.96 |
65.7±0.58 |
66.66±84 |
66.66±84 |
MCH
(µ/g) |
22.45±0.76 |
21.57±0.61
|
22.66±0.40 |
22.13±0.73 |
MCHC
(%) |
32.750.95 |
32.82±1.20 |
34.00±0.86 |
33.20±100 |
SEM = Standard error of
means; Hb= haemoglobin ; PCV = packed cell volume; WBC= white blood cell count
; MCV=mean corpuscular volume, MCH =mean corpuscular haemoglobin, MCHC = mean
corpuscular haemoglobin concentration
It
is hard to tell if this disparity is due to species differences or due to the
concentration of papain that could induce such disorders (as we could not
evaluate the papain concentrations of the diets). It is hoped that an analysis
of the papain concentration in pawpaw peels and the test diets will provide a
better understanding of the safe levels of inclusion of PPM in the diets of
female rabbits.
The non effect
of diet on the haematological characteristics of female rabbits obtained in
this study is in agreement with the earlier report of Bitto and Gemade (2001)
in male rabbits fed PPM. HB, PCV, and WBC values in female rabbits in the
present study were however generally lower than corresponding values reported
for male rabbits fed PPM (Bitto and Gemade, 2001). This disparity may be due to
differences in sex and age of animals, as sex and age (which differed between
the two reports) are among the factors reported to be responsible for
variations in haematological vales of rabbits (Mitruka and Rawnsley, 1977).
Laird et al (1970) earlier reported significant effects of age on some
haematological parameters in rabbits as well as significantly higher Hb and
hematocric values in males. This probably explains the lower values of Hb and
PCV in female rabbits fed PPM in the present study compared to males fed PPM in
an earlier report (Bitto ad Gemade, 2001). The lower leucocyte counts in female
rabbits in the present study compared to males fed PPM (Bitto and Gemade, 2001)
and normal values for males and females elsewhere (Mitruka and Rawnsley, 1977)
may in addition to other factors (age, sex and nutrition) be due to diurnal
variation which has been reported to considerably influence leucocyte count
(Mitruka and Rawnsley, 1977).
MCV,
MCH and MCHC values obtained in this study were however comparable to normal
values reported for both male and female rabbits (Mitruka and Rawnsley, 1977).
The red blood cells in these animals could therefore be classified as
normochromic, further confirming the suitability of PPM for the feeding of
female rabbits in the humid tropics.
CONCLUSION
We conclude from the results of this
study that even though PPM up to 30% level of inclusion may support growth, the
development of the reproduce tract and probably other body activities in female
rabbits, further work on the effect of PPM on other aspects of the physiology
of reproduction like endocrinology, the histometry of the reproductive organs
and the fertility of these animals may be required for the establishment of
optimum levels of PPM inclusion in the diets of female rabbits.
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