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Memórias do Instituto Oswaldo Cruz, Vol. 101, No.1, February 2006, pp. 95-97 Phenotypic characterization of three clinical isolates of Burkholderia pseudomallei in Ceará, Brazil CG Virginio/+, MFS Teixeira*, CC Frota, VS Café, MFG Rocha, JJC Sidrim Departamento de Patologia e Medicina Legal, Centro Especializado em Micologia Médica, Universidade Federal do Ceará, Rua Monsenhor Furtado s/nº, 60441-750 Fortaleza, CE, Brasil *Faculdade de Veterinária, Pós Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Fortaleza, CE, Brasil Financial support: Capes +Corresponding author: camila.virginio@secrel.com.br Received 11 May
2005 Code Number: oc06018 Burkholderia pseudomallei, the causative agent of melioidosis was found in a small cluster of cases in Tejuçuoca, Ceará, Brazil. Tests were carried out to determine its phenotypic characteristics: colony morphology on Ashdown agar and MacConkey agar, biochemical profile in conventional biochemical tests and API 20NE, arabinose assimilation and susceptibility testing by disk diffusion, comparing with data in the literature. This study confirms the presence of B. pseudomallei in Brazil and describes its characteristics. Key words: Burkholderia pseudomallei - melioidosis - Brazil Melioidosis is a serious human disease caused by Burkholderia pseudomallei. Isolation of B. pseudomallei from human samples had not been reported in Brazil until 2003 (Miralles et al. 2004, Braga & Almeida 2005). The purpose of this study was to compare the characteristics of these Brazilian isolates of the species with others reported in the literature, and to alert microbiologists in the region to these characteristics in order to enable them to recognize subsequent cases. In this study three isolates of B. pseudomallei were analyzed. These were isolated from clinical samples from a small cluster of cases that occurred in Tejuçuoca, Ceará, Brazil, involving three patients from the same family, aged 10-15 years old. Two strains of B. pseudomallei were initially isolated on MacConkey agar from the spleen, liver, and lung and one strain from blood culture in BHI broth. All procedures involving viable cells were performed in a category 3 containment laboratory. Strains were identified phenotipically according to the criteria described for gram-negative nonfermentative bacilli and by characteristics on Ashdown selective medium (Ashdown 1979, Gilligan & Whittier 1999, Koneman et al. 2001). The identification of B. pseudomallei was confirmed by biochemical tests using the API 20NE system (bioMérieux) (Dance et al. 1989). The bacteria were short, motile bipolar gram-negative bacilli. All three isolates showed characteristic bipolar staining, probably because of intracellular deposits of β-hydroxy butyric acid (Inglis et al. 2001, Sprague & Neubauer 2004). A thick and dry pellicle with a matt surface was seen on BHI broth cultures. This pellicle forms at the broth-air interface, possibly because of positive aerotaxis of B. pseudomallei, and reflects a type of a multi-cellular organization that resembles a biofilm. On MacConkey agar after 24 h of incubation the colonies were pink-transparent, small, smooth, with sheen and convex. After seven days of culture, isolates 1 and 3 showed larges, circulars, viscous, with sheen on surface and entire borders colonies. Isolate 2 also produced circulars, with surface sheen and pink colonies, however showed consistence dry and wrinkled. Ashdown's selective agar is commonly used to culture the organism from a mixed flora. All the B. pseudomal-lei isolates had characteristic colonial morphology on Ashdown agar. The colonies were smooth, moist, purple in color, dome shaped, about 0.8 to 1 mm in diameter, on plates after 24 h of incubation at 37ºC. After seven days of incubation isolate 2 grew as purple colonies, wrinkled, 5 to 6 mm in diameter, which had centrally umbonated, with radiating ridges at the periphery and a characteristic earthy odor; this showed a typical colonial morphology (Ashdown 1979). Isolates 1 and 3 included both smooth and mucoid colonies. The isolation of mucoid variant strains from clinical specimens is relatively uncommon (Inglis et al. 2001). Howard and Inglis (2003) have suggested that this may reflect inhibitory effect of crystal violet in Ashdown's agar. However, this not was observed in isolates 1 and 3. B. pseudomallei and a closely related species Burkholderia thailandensis, can be distinguished by their ability to assimilate the aldopentose L-arabinose. The L-arabinose non assimilators, B. pseudomallei, are highly virulent and can be isolated from both clinical specimens and the environment. The L-arabinose assimilators, now known as B. thailandensis, however, are generally avirulent and found predominantly in the environment (Brett et al. 1998). B. pseudomallei isolates were tested for arabinose assimilation by growth on minimal salts agar containing 0.2% L-arabinose (Wuthiekanun et al. 1996). None of the clinical isolates of B. pseudomallei could assimilate arabinose, which was demonstrated by absence of growth on minimal salts medium in 48 h. Although Ara+ B. pseudomallei has been reported to cause infection (Lertpatanasuwan et al. 1999), all isolates causing melioidosis in our study were Ara. Our findings support previous observations described by Vuddhakul et al. (1999) in Thailand and by Miralles et al. (2004) in Brazil. In this study, the results of the biochemical profile in the OF medium tests with lactose, maltose, and mannitol for isolates 1 and 3 differed from those Gilligan and Whittier (1999), but this may reflect differences in methodology utilized for assimilation tests, rather than genuine differences in Brazilian isolates. All the B. pseudomallei isolates were identified correctly by API 20NE in 48 h. The profile obtained for isolates 1, 2, and 3 were 1556577 (99.1% correct identification), 1156577 and 1156577 (99.9% correct identification) respectively. In API 20NE strips, differences in esculin hydrolysis were observed (isolate 1 was positive, isolates 2 and 3 were negative), although this did not affect the identification profile, as esculin hydrolysis by B. pseudomallei is quite variable (Gilligan & Whittier 1999, Koneman et al. 2001). Susceptibility tests were undertaken with the following antibiotics: piperacilin-tazobactam, ticarcilin-clavulanate, ceftazidime, ceftriaxone, cephalotin, imipenem, gentamicin, tetracycline, ciprofloxacin, chloramphenicol, and sulfamethoxazole-trimethoprim (Cecon®). All antibiotics were tested by disk diffusion using standard methods (NCCLS 2003). The results of susceptibility tests are shown in Table and were typical of B. pseudomallei. In general, the Brazilian isolates exhibited similar profile to those other parts of the world. Our report confirms previous studies as realized by Gales et al. (2005) for in vitro susceptibility Burkholderia species. However, in this study, our isolates were resistant to sulfamethoxazole-trimethoprim, although this may have occurred by differences in methodology utilized. B. pseudomallei is known to produce a number of beta-lactamases (Niumsup & Wuthiekanun 2002). These results mean that the current recommendations for the treatment of melioidosis can also be applied to case from Brazil (CDC 2004). ACKNOWLEDGEMENTS To Universidade Federal do Ceará, Department of Microbiology, Faculty of Medicine, for technical support. REFERENCES
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