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Arylfurans as potential Trypanosoma cruzi trypanothione reductase inhibitors Renata B de Oliveira, Aline BM Vaz, Rosana O Alves*, Daniel B Liarte*, Claudio L Donnici**, Alvaro J Romanha*, Carlos L Zani/+ Laboratório de Química de Produtos Naturais *Laboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou-Fiocruz, Av. Augusto de Lima 1715, 30190-002 Belo Horizonte, MG, Brasil **NEQUIM - Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil Financial support: Fiocruz, CNPq, Fapemig +Corresponding author. E-mail: zani@cpqrr.fiocruz.br Received 4
November 2005 Code Number: oc06032 The natural lignans veraguensin and grandisin have been reported to be active against Trypanosoma cruzi bloodstream forms. Aiming at the total synthesis of these and related compounds, we prepared three 2-arylfurans and eight 2,5-diarylfurans. They were evaluated for their potential as T. cruzi trypanothione reductase (TR) inhibitors as well against the parasite's intracellular (amastigote) and bloodstream (trypomastigote) forms. Compound 12 was the most effective against TR with an IC50 of 48.5 µM while 7 and 14 were active against amastigotes, inhibiting the parasite development by 60% at 20 µg/ml (59 and 90 µM, respectively). On the other hand, none of the compounds was significantly active against the parasite bloodstream forms even at 250 µg/ml (0.6-1.5 mM). Key words: tropical diseases - Chagas disease - arylfurans - trypanothione reductase Chagas disease, caused by the flagellate protozoan Trypanosoma cruzi, affects 18 million people in Latin America and is responsible for 13,000 deaths every year (WHO 2002). The treatment relies on only two available drugs, nifurtimox and benznidazole, which are relatively efficient in the acute phase of the disease, but almost ineffective in the chronic phase. Nowadays, one of the most important mechanisms of Chagas disease transmission in many countries is by blood transfusion (Schmuñis 1991). In highly endemic areas it is strongly recommended the use of chemoprophylatic measures such as the addition of gentian violet to clear trypomastigotes from blood banked for transfusion (Moraes-Souza et al. 1995). Although effective, this triphenylmethane dye is not well accepted because of undesirable effects such as coloring the skin and possible mutagenicity (Wendel 1993). Thus, new drugs to treat or prevent Chagas disease are still needed. Trypanosoma cruzi enzymes such as the trypa-nothione reductase (TR) represent a potential drug targets because they play an essential role in the life of this parasite. TR and its substrate trypanothione, the disulfide of a glutathione-spermidine conjugate [N1, N8-bis(glutathionyl)spermidine, T(SH)2] 1, help to protect the parasite from oxidative stress by maintaining an intracellular reducing environment in a manner analogous to glutathione reductase (GR) and glutathione [L-g-glutamyl-L-cysteiylglycine, GSH] 2 (Fig. 1a) in mammalian cells (Schmidt & Krauth-Siegel 2002). TR catalyses the NADPH-dependent reduction of trypanothione disulfide TS2 to its dithiol form, T(SH)2 . Trypanothione may be oxidized back to TS2 (Fig. 1b) following reaction with potentially damaging radicals and oxidants generated by aerobic metabolism. Another aspect that makes TR an even more attractive target is its structural differences from the human counterpart GR. GR has a narrow positively charged active site, to accommodate the glycine carboxylates of its substrate glutathione, whereas TR has a wider, non-charged, and more hydrophobic active site (Bond et al. 1999). These differences allowed the discovery of several promising selective inhibitors of TR (Schmidt & Krauth-Siegel 2002). Lignans is a class of natural products that possess important biological properties (Jensen et al. 1993). Lopes et al. (1998) showed that the tetrahydrofuran lignans veraguensin 3 and grandisin 4 (Fig. 2) were active in vitro at 5 µg/ml against the trypomastigote of T. cruzi present in murine blood, causing 100% of parasite lysis without damaging erythrocytes. The activity of these lignans was fifty times higher than that of the reference drug gentian violet. Based on these promising results, we decided to synthesize these natural products and some analogues to evaluate their activity against T. cruzi. Our synthetic route to 3 and 4 (Fig. 3) involved arylfurans as intermediates. Arylfurans present a broad-spectrum of biological activities [for example, antimicrobial activity (Stephens et al. 2001, Lanteri et al. 2004), effects against neurodegen-erative, cardiovascular, and proliferative diseases (Lockhart et al. 2004), inhibitory activity against the enzymes farnesyltransferase (Mitsch et al. 2004) and PDE4 (Perrier et al. 1999), vascularization inhibitor effect (Kuwano et al. 1994)] and previous works (Jockers-Scherübl et al. 1989, Paulino et al. 2002, Aguirre et al. 2004) have shown that furan derivatives could be potential ligands for TR. In view of these results, and aiming at the discovery of new trypanocidal compounds we evaluated the effect of the synthetic compounds 5-15 (Fig. 4) on TR and the whole trypomastigote and amastigote forms of the parasite. MATERIALS AND METHODS Chemistry - The 2-arylfurans 5-7 and the 2,5-Bis(p-cyanophenyl)furan 9 were synthesized in one step using the classical Meerwein arylation (treatment of furan with diazonium salts in the presence of cupric salts) (Rondestvedt 1976). The 2,5-diarylfurans 8, 10-15 were prepared in two steps: (1) preparation of 2,5-bis (trimethylstannyl)furan 16 by reaction of furan with TMEDA and n-butyllithium and subsequent addition of trimethyltin chloride (Seitz et al. 1983), and (2) palladium catalyzed coupling reaction between the distannane 16 and various arylhalides (Stille coupling) (Stephens et al. 2001). In vitro assay with T. cruzi TR - Recombinant T. cruzi TR was obtained as described by Borges et al. (1995). The colorimetric microtitre plate assay was adapted from that described by Hamilton et al. (2003). It was run in 40 mM HEPES (pH 7.5), 1 mM EDTA, 0.12 mM NADPH and 0.8 µM trypanothione TS2 in a total assay volume of 250 µl. The test compounds were dissolved in DMSO and diluted with water to a final concentration of 0.1% (v/v) DMSO. After addition of the enzyme (5.12 mU/well) to the compound solution (20 µg/ml, 50-120 µM) the mixture was incubated for 30 min at 30ºC, after which 25 µM of 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) was added and the absorbance at 410 nm measured in a kinetic mode for 5 min. Clomipramine at its IC50 (6.5 µM) was used as positive and 0.1% (v/v) DMSO as negative controls. Each compound was tested in triplicate. The results are expressed as percentage reduction of the TR activity. For IC50 determinations, inhibition assays were carried out at ten different concentrations and repeated four times. In vitro assay with T. cruzi blood stream forms - The assays with T. cruzi were carried out using blood from Swiss albino mice collected in the parasitaemia peak (7th day) after infection with the Y strain of T. cruzi. The infected blood was diluted with normal murine blood and RPMI 1640 medium 1:2 (pH 7.2-7.4) to the concentration of 2 × 106 trypomastigotes/ml. Stock solutions at 10 mg/ml (25-59 mM) of the compounds were prepared in dimethylsulfoxide (DMSO). A sample (5.0 µl) of each solution was added to 195 µl of infected blood providing a final concentration of 250 µg/ml (0.6-1.5 mM). Samples of 100 µl were transferred in duplicate to the wells of a microtitre plate (96 wells). To reproduce the blood bank conditions, plates were incubated at 4ºC for 24 h. The experiments were repeated two times. Afterwards, the parasite concentration was evaluated using an optical microscope with a 400 X magnification. DMSO at 2.5% v/v and gentian violet at its IC50 concentration (7.5 µg/ml) were used as negative and positive controls, respectively. DMSO was not added in the positive control. The parasite concentration reduction (parasite lysis) was determined in comparison with negative control containing only 2.5% DMSO. At 2.5% concentration DMSO in blood was found to cause no morphological alterations or lysis either in the parasites, erythrocytes or leukocytes. In vitro assay with T. cruzi amastigote forms (Buckner et al. 1996 modified) - Parasites and culture procedures: T. cruzi (Tulahuen strain) expressing the Escherichia coli beta-galactosidase gene were grown on monolayer of mouse L929 fibroblasts. Cultures to be assayed for beta-galactosidase activity were grown in RPMI 1640 medium (pH 7.2-7.4 ) without phenol red (Gibco BRL) plus 10% fetal bovine serum, glutamine and gentamicin. T. cruzi growth inhibition assay - Ninety-six-well tissue culture plates were seeded with L929 fibroblasts at 4.0 × 103 per well in 80 µl and incubated overnight. Beta-galactosidase-expressing trypomastigotes were then added at 4.0 × 104 per well in 20 µl. After 2 h, the medium with trypomastigotes that not penetrated in cells was discarded and replaced by 200 µl of fresh medium. After 48 h, the medium was discarded again and replaced by 180 µl of fresh medium and test compounds in 20 µl. Each compound was tested in triplicate. After 7 days of incubation, chlorophenol red beta-D-galactopyranoside (CPRG) (100 mM final concentration) and Nonidet P-40 (0.1% final concentration) were added to the plates and incubated overnight at 37°C and the absorbance measured at 570 nm in an automated micro plate reader. Benznidazole at its IC50 (4.0 µM) was used as positive control. The results are expressed as percentage growth inhibition. RESULTS AND DISCUSSION The monoarylfurans 5-7, and the diarylfurans 9 were synthesized in a single step via the Meerwein arylation in moderate yields. Initial attempts to prepare 2,5-diarylfurans by this method were unsuccessful and only mono-arylfurans were formed, except for compound 9, obtained with 12% yield. Under this reaction condition anilines containing electron donor substituents attached to the phenyl ring furnished only tarry material from which neither mono nor diarylfurans could be isolated. Therefore, the 2,5-diarylfurans 8, 10-15 were prepared in two steps using the Stille coupling. The structures of all compounds were confirmed by spectroscopy and physicochemical data (Table I). Table II shows the results of the bioassays using the pure compounds 5-15 against TR and T. cruzi trypomastigote and amastigote forms. For the comparison of the activities standard control drugs were included in the assays. The compounds were tested for inhibition of TR at 20 µg/ml and the IC50 values were determined for the most active compounds. Thus, the nitro derivative 6 and the diacetamide 12 presented IC50 values of 155 µM and 48.5 µM, respectively. Many nitrofuran derivatives have been reported to act as subversive substrate for TR, a class of inhibitors that produce free radicals once reduced by the enzyme, thereby subverting its physiological role (Jockers-Scherübl et al. 1989, Paulino et al. 2002, Maya et al. 2003, Chibale & Musonda 2003, Aguirre el al. 2004). In the presence of oxygen, these inhibitors are cyclically reduced and reoxidized generating deleterious oxygen radicals while simultaneously inhibiting TR's ability to reduce its physiological substrate (Chibale & Musonda 2003). Thus, the inhibitory activity presented by the nitro derivative 6 could be associated to its ability to act as subversive substrates. On the other hand the related diarylfuran 10, despite containing two NO2 groups, was less active than 6, probably due to its poor solubility in the assay medium. The diacetamide 12 was the best inhibitor and its activity may come from to the combination of the hydrophobic moiety with the amide groups. The amide groups may be important for the interaction between compound 12 and the enzyme via hydrogen bonds. However, further experimental evidences are needed to confirm such possibility. The results in Table II show no correlation between enzyme inhibition and trypanocidal activity as the two major TR inhibitors, compounds 6 and 12, showed no effect against intact bloodstream or amastigote forms of the parasite. On the other hand, compound 15, inactive against TR, reduced by 41% the number of trypo-mastigotes in the blood while compounds 7 and 14 were able to inhibit by more than 60% the growth of intracellular amastigotes. Interestingly compound 14 is structurally related with the lignan veraguensin 3. Table II shows the calculated partition coefficients for the arylfurans 5-15. Partition coefficient between water and octanol (log P) is regarded as a measure of lipophilicity of a drug and is related to its ability to cross biological membranes. Substances with high log P values dissolve better in fats and oils than in water. This enhances their ability to enter lipid membranes by passive diffusion, thereby enhancing their potential for absorption. Generally, for a good activity the relationship appears to be parabolic with an optimum Log P value of around 2 ± 1. It is well known that compounds with negative log P values cross cell membranes very poorly (Lipinski et al. 1997). The log P values for compounds 11 and 15 are outside the optimum limits. Compound 6 has a reasonable log P value but its IC50 is so high that an effective concentration inside the parasite would be difficult to reach. The low trypanocidal activity of the diamide 12 may be due to factors such as a) poor membrane permeability as indicated by its low log P value (log P = 0.39); b) the in vitro assay with T. cruzi bloodstream forms mimics blood bank conditions and is carried in a short assay time (24 h) and low temperature (4ºC), making it difficult to detect the interference of the compound on the TR activity; c) In the intracellular assay with amastigotes the compound 12 could not be able to cross the fibroblast and parasite's cell membranes to reach parasite's cytoplasm in sufficient quantity to significantly inhibit TR. Therefore, a direct correlation between the trypanocidal activity and inhibition of TR by the compounds was not observed. Indeed, even clomipramine, one of the most potent inhibitor of TR presented only moderate activity (72% inhibition) against intracellular amastigote forms at 57 µM (20 µg/ml). Despite its failure to reduce parasites under the conditions used in the present work, compound 12 deserves further investigation to determine its TR inhibition mechanism and to develop related compounds with improved potency. ACKNOWLEDGEMENTS To Prof. Alan Fairlamb, University of Dundee, Scotland, for providing the recombinant T. cruzi TR and to Prof. Frederick S Buckner, Univdersity of Washington, for providing the Tulahuen strain of T. cruzi expressing the E. coli beta-galactosidase gene used in this work. REFERENCES
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