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Memórias do Instituto Oswaldo Cruz, Vol. 105, No. 8, 2010, pp. 1054-1056 SHORT COMMUNICATIONS Paleoparasitologic, paleogenetic and paleobotanic analysis of XVIII century coprolites from the church La Concepción in Santa Cruz de Tenerife, Canary Islands, Spain Herminia Gijón BotellaI; José A Afonso VargasII; Matilde Arnay de la RosaII; Daniela LelesIII, IV; Emilio González ReimersV; Ana Carolina P VicenteIII; Alena M IñiguezIII, + IDepartamento
de Parasitología, Facultad de Farmacia, Universidad de Granada, Granada,
España Received 10 June
2010 Code number: oc10180 ABSTRACT We present the results of a paleoparasitologic, paleogenetic and paleobotanic analysis of coprolites recovered during the excavation of the church La Concepción in Santa Cruz de Tenerife. Coprolites (n = 4) were rehydrated and a multidisciplinary analysis was conducted. The paleobotanic analysis showed numerous silicates, seeds and fruits of the family Moraceae. In the paleoparasitologic study, Ascaris sp. eggs (n = 344) were identified. The paleogenetic results confirmed the Ascaris sp. infection as well as the European origin of human remains. These findings contribute to our knowledge of ancient helminthes infections and are the first paleoparasitological record of Ascaris sp. infection in Spain. Key words: paleoparasitology - Ascaris sp. - paleobotany - coprolite - paleogenetic - Canary Islands - ancient DNA La Concepción was the major church of the ancient village of Santa Cruz de Tenerife in the centuries following the Spanish conquest. The church was not only the most important in the region, but also served as the community's cemetery. Following the Catholic liturgy, the dead were buried in the church foundation, a practice which began in the XVI century and persisted until the end of the XVIII century. The location of La Concepción near the seashore led to the relatively poor preservation of skeletal remains, but some archaeological studies have been possible (Gámez-Mendoza 2004, Arnay-de-la-Rosa et al. 2009). During the excavation, both bones and coprolites were recovered, which allowed us to conduct a multidisciplinary analysis. Samples of coprolites (n = 4) were removed from tomb 146 (Fig. 1A), which contained a young female, whose sex was determined by cranial characteristics and whose age at death was determined by the absence of dental attrition (Ubelaker 1989). Coprolites were rehydrated by immersion in 0.5% aqueous trisodium phosphate for 72 h, as described by Callen and Cameron (1960) (Fig. 1B). Seeds of fruits belonging to the family Moraceae were clearly visible in the coprolite. Microscopic investigation was performed using the methods described by Danielson and Reinhard (1998). This revealed numerous phytoliths derived from the silicated external envelopes of crop grains (silica skeletons) and starch grains corresponding to vegetables in the family Triticeae, which includes wheat and barley, indicating the presence of cereals in the diet. The paleoparasitologic investigation was conducted by microscopic analysis prior to the spontaneous sedimentation (Lutz 1919) of rehydrated coprolite solutions. We examined 20 slides for the presence of parasite eggs at magnifications of 100X and 400X. The results revealed the presence of Ascaris sp. eggs (Fig. 1C). The eggs measured 57.92-70.1 μm in length and 47.14-49.25 μm in width (n = 344). To ensure that authentic original sequences were analyzed by paleogenetic analysis, precautions to prevent contamination by modern DNA were followed as described by Drancourt and Raoult (2005). The preparation of sample, ancient DNA extraction and (aDNA)polymerase chain reaction (PCR) were performed at the Laboratory of Paleogenetics, Oswaldo Cruz Institute/Oswaldo Cruz Foundation (IOC/FIOCRUZ). Electrophoresis, cloning, sequencing and sequence analysis were conducted at the Laboratory of Molecular Genetics of Microorganisms, IOC/FIOCRUZ. These two laboratories are physically distant from each other. The surface of the coprolites was removed, samples were irradiated with ultraviolet light and the aDNA was extracted as described by Iñiguez et al. (2003, 2006). The sediments of nine coprolites were submitted for molecular investigation. Human and Ascaris sp. targets were analyzed by procedures described by Fernandes et al. (2008) and Leles et al. (2008), respectively. For Ascaris sp. analysis, the primers of 18S ribosomal DNA (rDNA) gene were used and others were designed based on the NADH dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) genes (Table). PCR products were directly sequenced in both directions using a 3100 Automated DNA Sequencer (Applied Biosystems, Life Technologies, Foster City, CA, USA). The nucleotide sequences were analyzed with Lasergene© SeqMan v.7.0.0 (DNASTAR, Madison, WI, USA) and Bioedit v.5.0.9 (Department of Microbiology, North Carolina State University, USA) softwares. The human mitochondrial DNA (mtDNA) sequence was compared to mtDNA database from GenBank and from laboratory staff. We successfully recovered a segment of human mtDNA HVS-I region (16209-16356) and the European origin of the sample was confirmed based on signatures HVSI genetic (16224 and 16311) characteristic of haplogroup K. Ascaris sp. PCR products from nad1 and cox1 were obtained, but the sequencing analysis revealed unspecific amplicons. However, the 18S rDNA target was useful in yielding aDNA fragments corresponding to the Ascaris sp. gene in two different sediments. The assembly of aDNA fragments allowed the authentication of the 18S rDNA amplicons (Fig. 2). According to Gonçalves et al. (2003), Ascaris sp. eggs have been found in 39 European archaeological sites from 11 countries, excluding Spain. In this study we showed for the first time, by paleoparasitologic and paleogenetic analysis, that the occurrence of ascariasis in ancient times includes the Canary Islands, Spain. Despite the identification of human remains and human mtDNA haplotype recovery, we could not determine the species of Ascaris sp. parasite as Ascaris lumbricoides, the species of human origin, because the 18S rDNA gene does not discriminate to this taxonomic level. Nuclear and mitochondrial genes have been proposed to discriminate species of Ascaris, but the genetic definition between these closely related forms remains unclear (Anderson et al. 2001, Leles et al. 2010). The first molecular paleoparasitologic study of Ascaris sp. reported the recovery of aDNA from a concentrate of parasite eggs from the Middle Age site Place d´Armes in Namur (XIV century) (Loreille et al. 2001). Later, a sample from Walraversijde, Belgium (XVI century), which was used as positive control on South American paleogenetic analyses, showed a segment of cyt b gene from aDNA directly extracted from coprolites (Leles et al. 2008). In this study, we confirm the ability of this methodology to recover aDNA without the prior concentration of parasite eggs (Iñiguez et al. 2003, 2006, Leles et al. 2008). This paleoanalysis, based on an interdisciplinary approach, is the first of its kind performed in archaeological samples from the Canary Islands. ACKNOWLEDGEMENTS To Koko Otsuki, from the Laboratory of Molecular Genetics of Microorganisms IOC-FIOCRUZ, Rio de Janeiro, for her excellent technical assistance, and PDTIS/FIOCRUZ genomic platform for nucleotide sequencing. REFERENCES
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