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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 92, Num. 2, 1997, pp. 233-234
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Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 92(2),
Mar/Apr 1997, pp.233-234
Research Note
First Record of Anopheles benarrochi Gabaldon, Cova Garcia & Lopez from
the State of Sao Paulo, Southern Brazil
Maria Anice Mureb Sallum, Eduardo Sterlino Bergo*, Oswaldo Paulo
Forattini/+
Departamento de Epidemiologia, Nucleo de Pesquisa Taxonomica e
Sistematica em Entomologia Medica, Faculdade de Saude Publica, Universidade
de Sao Paulo, Av. Dr. Arnaldo 715, 01246-904 Sao Paulo, SP, Brasil
*Superintendência de Controle de Endemias (SUCEN), Rua Italia 1617,
14801-350, Araraquara, SP, Brasil
+Corresponding author. Fax: +55-11-282.1898
Received 26 August 1996
Accepted 30 October 1996
Code Number:OC97046
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Key words: Anopheles benarrochi - first record - State of
Sao Paulo - Brazil
According to ME Faran (1980 Contrib Am Entomol Inst 15:1-215) the
distribution range of Anopheles benarrochi Gabaldon, Cova Garcia &
Lopez is limited to the Orinoco basin and eastern versant of the Andes
including the llanos plateau region of Colombia, localities of the upper
Amazon in Brazil (Rondonia, Acre, Amazonas) and Loreto, Peru. While
conducting ecological studies in Bocaina, near Araraquara City, State of
Sao Paulo, Brazil, it was possible to raise progeny broods of female of
several species of Anopheles (Nyssorhynchus). Among the families we
raised, one of them was identified as An. benarrochi which is, for
the first time, recorded in the State of Sao Paulo.
Females Anopheles were collected on human bait, in open land,
near edge vegetation, at Jacare-Pepira River, Santa Leonor Farm, Route SP
255 km 125, Bocaina, near Araraquara, State of Sao Paulo (22 degrees 04'19"
S and 48 degrees 26'47"W), about 420 m above sea level, in a man-made
environment with intense agricultural activities, mainly sugar-cane and
pasture. The remainder natural vegetation corresponds to edge vegetation
along the Jacare-Pepira River, patches of savanna and tropical deciduous
forest. According to the climatic classification of Koeppen (J Setzer 1966
Atlas climatico do Estado de Sao Paulo, Sao Paulo, Comissao
Interestadual da Bacia Parana - Uruguai - Centrais Eletricas de Sao Paulo)
the climate is subtropical humid. The collections were made on human bait
during the dusk hours following methodology reported by OP Forattini et al.
(1981 Rev Saude Publ 15: 557-586), on 3rd July 1995. Females of
several species of Anopheles were collected besides that of An.
benarrochi and identified as An. darlingi Root, An.
evansae (Brethes), An. galvaoi Causey, Deane & Deane, An.
rondoni (Neiva & Pinto), An. strodei Root, An.
triannulatus (Neiva & Pinto) and An. albitarsis l.s.
The adult female of An. benarrochi was misidentified as An.
strodei. Females were fed and left to lay eggs in small glass vials
with wet cotton and filter paper on the bottom. After eclosion the larvae
were raised to the adult stage in order to have adults associated with
larval and pupal exuviae. Specimens were identified using Forattini's (1962
Entomologia Medica, v.1, Sao Paulo, Fac. Higiene e Saude Publica,
662 pp.), Faran's (loc. cit.) and RAGB Consoli and R
Lourenco-de-Oliveira's (1994 Principais mosquitos de importancia
sanitaria no Brasil. Ed. Fiocruz, Rio de Janeiro, 225 pp.) keys.
According to Faran (loc. cit.) the adult female of An.
benarrochi is indistinguishable from that of An. aquasalis
Curry. Although similar to An. strodei it can be separated from
An. strodei by having hindtarsomere 2 dark in about basal half
(0.3-0.6 basal), light wing spots at least on veins C and R light cream to
yellowish, never white. In An. strodei the hind tarsomere 2 is dark
in less than basal 0.4, or when greater than 0.4 then the light wing spots
are white, never light cream to yellowish. The author emphasized that a
great care should be taken when examining specimens of An.
benarrochi from other localities because he examined only few
individuals.
Consoli and Lourenco-de-Oliveira (loc. cit.) considered that the
adult female of An. benarrochi can be distinguished from those of
An. strodei, An. aquasalis, An. galvaoi and An. evansae by
possessing vein M mostly dark-scaled at least on ventral surface, and in
the other species it is mostly white-scaled. Faran's and Consoli and
Lourenco-de-Oliveira's keys do not allow one to identify An.
benarrochi from Bocaina, State of Sao Paulo. In Faran's key individuals
of An. benarrochi can be misidentified as An. strodei and in
Consoli and Lourenco-de-Oliveira's key as An. galvaoi.
However, An. benarrochi can be easily distinguished from An.
strodei, An. galvaoi and An. aquasalis by characters of
the male genitalia and immature stages. Faran (loc. cit.) did not
include in his key the immature stages of An. galvaoi, An.
rondoni and An. anomalophylus Komp. The later species has not
been found in Brazil, and according to Wilkerson and Strickman (1990 J
Am Mosq Control Assoc 6: 7-34) its distribution is limited to Central
America. The other two species are known from several localities in the
State of Sao Paulo as well as in other localities throughout Brazil. In
Consoli and Lourenco-de-Oliveira's and Faran's keys the larva of An.
benarrochi can be separated from those of similar species by having
seta 3-C with moderately long to long branches, in the other species seta
3-C is single or with minute aciculae. Faran (loc. cit.) did not
include the pupae of An. galvaoi and An. rondoni in his key,
however, he considered that the pupa of An. benarrochi can be
recognized by having seta 9-VII short, less than or equal to 0.33 length of
segment, pinna 4.4-5.5 length of meatus, not appearing to taper toward apex
in lateral aspect, meatal cleft moderately pointed to rounded at base, seta
6-II much longer than 7-II, 10-C subequal to 11-C and more than 0.5 length
of 12-C and 6-I 1.5-2.0 length of 7-I.
Although difficult to recognize adult female of An. benarrochi
from State of Sao Paulo using Faran's and Consoli and
Lourenco-de-Oliveira's adult keys, this species can be easily identified in
the larval and pupal stages and male genitalia. As a conclusion, the
progeny broods of females collected in Bocaina, State of Sao Paulo, were
identified as An. benarrochi by characters of larva, pupa and male
genitalia.
Partially supported by grant no.95/7157-2, Fundacao de Amparo a Pesquisa
do Estado de Sao Paulo.
Copyright 1997 Fundacao Oswaldo Cruz
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