Indian Journal of Pharmacology Medknow Publications on behalf of Indian Pharmacological Society ISSN: 0253-7613 EISSN: 1998-3751 Vol. 40, Num. 1, 2008, pp. 19-22

Indian Journal of Pharmacology, Vol. 40, No. 1, January-February, 2008, pp. 19-22

Research Article

Antidiabetic activity of aqueous root extract of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetes in rats

Barik Rakesh, Jain Sanjay, Qwatra Deep, Joshi Amit, Tripathi GirrajSharan, Goyal Ravi

Department of Pharmacognosy, Smriti College of Pharmaceutical Education, 4/1, Pipliya Kakkad, Mayakhedi Road, Nipania, Indore - 452 010, MP
Correspondence Address:Department of Pharmacognosy, Smriti College of Pharmaceutical Education, 4/1, Pipliya Kakkad, Mayakhedi Road, Nipania, Indore - 452 010, MP
barikrakesh@yahoo.co.uk

Date of Submission: 23-Aug-2007
Date of Decision: 10-Feb-2008
Date of Acceptance: 07-Mar-2008

Code Number: ph08005

Abstract

Keywords: Antidiabetic oral glucose tolerance test, Ichnocarpus frutescens, streptozotocin-nicotinamide induced type-II diabetes mellitus

Diabetes mellitus is a metabolic disorder characterized by disturbances in carbohydrate, protein, and lipid metabolism and by complications like retinopathy, microangiopathy, and nephropathy. ^[1] Currently available synthetic antidiabetic agents produce serious side effects like hypoglycemic coma ^[2] and hepatorenal disturbances. ^[3] Moreover they are not safe for use during pregnancy. ^[4] Hence, the search for safer and more effective hypoglycemic agents has continued. Following the WHO′s recommendation for research on the beneficial uses of medicinal plants in the treatment of diabetes mellitus, ^[5] investigations on hypoglycemic agents derived from medicinal plants have also gained momentum. Several investigations have been conducted and many plants have shown a positive activity. ^[6] Though the active principles have been isolated from some plants, some still remain to be identified.

Ichnocarpus frutescens (Apocynaceae), commonly known as black creeper and ′ dudhilata ′ is found all over India. The roots are used as a diuretic and diaphoretic. ^[7] The stalks and leaves are used in the treatment of skin eruptions and fever. ^[8] The tribals of Madhya Pradesh ^[9] use the roots and the siddis of Uttara Kannada district of Karnataka ^[10] use the roots and flowers as a cure for diabetes. So far, however, there is no scientific evidence to support the antidiabetic effect of Ichnocarpus frutescens (IF). The objective of this study was to ascertain the scientific basis for the use of this plant in the management of diabetes using streptozotocin-nicotinamide induced type-II diabetic rats.

Materials and Methods

Collection of plant material
The roots of IF were collected from the local market in Madhya Pradesh and identified by Dr. S.C. Jena, Director, Chattisgarh Rajya Van Vikas Nigam Limited, Madhya Pradesh, and Mr. A. K. Murthy, Medicinal Plants Survey, Central Ayurvedic Research Institute, Bhubaneswar, Orissa.

Preparation of aqueous root extract of IF
The aqueous extract was prepared by cold maceration of 150 g of the shade-dried roots′ powder in 500 ml of drinking water for 7 days. The extract was filtered, concentrated, dried in vacuo (yield 65 g), and the residue stored in a refrigerator at 2-8°C for use in the experiments. ^[11]

Animals
Healthy adult male Wistar albino rats between 2-3 months of age and weighing 250-280 g were used for the study. Housed individually in polypropylene cages, maintained under standard conditions (12-h light and 12-h dark cycle; 25 ± 5°C; 35-60% humidity), the animals were fed with standard rat pellet diet (Hindustan Lever Ltd, Mumbai, India) and provided water ad libitum .

Acute toxicity study
Animals were starved overnight and divided into five groups ( n = 6). They were fed orally with the aqueous extract of IF in increasing dose levels of 100, 500, 1000, 3000, and 5000 mg/kg body weight. The animals were observed continuously for 2 h for the following: ^[12]

i. Behavioral profile: alertness, restlessness, irritability, and fearfulness
ii. Neurological profile: spontaneous activity, reactivity, touches response, pain response, and gait
iii. Autonomic profile: defecation and urination

The number of deaths, if any were recorded after 24 and 72 h.

Induction of non-insulin dependent diabetes mellitus (NIDDM)
NIDDM was induced ^[13] by a single intraperitoneal injection of 60 mg/kg streptozotocin (Sigma Aldrich, Germany) followed by nicotinamide (Ranbaxy Chemicals Ltd, Mumbai, India) 120 mg/kg, i.p, 15 min afterwards. Streptozotocin (STZ) was dissolved in citrate buffer (pH 4.5) and nicotinamide was dissolved in normal saline. ^[14] Hyperglycemia was confirmed by the elevated glucose levels in plasma, determined at 72 h and then on day 7 after injection. The threshold value of fasting plasma glucose to diagnose diabetes was taken as> 126 mg/dl. ^[15] Only those rats that were found to have permanent NIDDM were used for the study.

Collection of blood and determination of serum glucose
Blood was withdrawn from the retroorbital sinus under ether inhalation anesthesia and glucose levels were estimated using a glucose oxidase-peroxidase reactive strips and a glucometer (Accu-Chek, Roche Diagnostics, USA).

Oral glucose tolerance test (OGTT)
The oral glucose tolerance test ^[16] was performed in overnight (18-h) fasted normal rats. Rats divided into four groups ( n = 6) were administered either drinking water or IF aqueous extract, 250 and 500 mg/kg, respectively. Glucose (2 g/kg) was fed 30 min after the administration of the extract. Glibenclamide (10 mg/kg) was used as the standard drug. Blood was withdrawn from the retroorbital sinus under ether inhalation anesthesia at 30, 60, and 120 min of glucose administration and glucose levels were estimated using glucose oxidase-peroxidase reactive strips and a glucometer (Accu-Chek, Roche Diagnostics, USA).

Experimental design for antidiabetic study
Overnight fasted diabetic rats were divided for the antidiabetic study in the following manner: Group I: diabetic control rats administered gum acacia daily for 15 days; Group II: diabetic rats administered IF aqueous extract (250 mg/kg); Group III: diabetic rats administered IF aqueous extract (500 mg/kg); Group IV: diabetic rats administered the standard drug glibenclamide (0.25 mg/kg) for 15 days.

The fasting blood glucose levels were determined on day 0, 10, and 15. During the experimental period, the rats were weighed daily and the mean change in body weight was calculated.

Statistical analysis
Data were statistically evaluated using one-way ANOVA, followed by post hoc Sheffe′s test using SPSS, version 7.5. The values were considered significant when P < 0.05. ^[17]

Results

Effect of IF aqueous root extract on acute toxicity study
Acute toxicity studies revealed the nontoxic nature of the aqueous root extract of IF . There were no lethality or toxic reactions found at any of the doses selected until the end of the study period. All the animals were alive, healthy, and active during the observation period.

Effect of IF aqueous root extract on oral glucose tolerance test
In OGTT, the aqueous extract induced significant reduction in plasma glucose levels from 30 min onwards [Table - 1].

Effect of IF aqueous root extract on fasting blood sugar level
On repeated administration of the extract for 15 days, a significant decrease in the fasting blood sugar level was observed in the diabetic rats as compared to the control [Table - 2].

Effect of IF aqueous root extract on changes in body weight
Diabetic rats showed a decrease in body weight during the experimental period; this was significantly antagonized by the extract [Table - 3].

Discussion

The present work has detected the antidiabetic effect of the aqueous roots extract of Ichnocarpus frutescens in streptozotocin-nicotinamide induced type-II diabetic rats. Streptozotocin-nicotinamide injection caused diabetes mellitus, probably due to destruction of the β-cells of the islets of Langerhans of the pancreas. ^[18] Over-production of glucose and decreased utilization by the tissues form the fundamental basis of hyperglycemia in diabetes mellitus. ^[19]

When IF aqueous roots extract was administered to glucose-loaded normal rats, hypoglycemia was observed after 30 min, with the maximum effect being seen at 2 h. Our investigations also indicate the efficacy of the aqueous extract in the maintenance of blood glucose levels in normal and streptozotocin-nicotinamide induced diabetic rats. Induction of diabetes with STZ is associated with a characteristic loss of body weight, which is due to increased muscle wasting ^[20] and loss of tissue proteins. ^[21] Diabetic rats treated with the IF extract showed an increase in body weight as compared to the diabetic control, which may be due to its effect in controlling muscle wasting, i.e., by reversal of antagonizing. ^[22]

Conclusion

Acknowledgment

References

Copyright 2008 - Indian Journal of Pharmacology

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