Revista Colombia Médica Universidad del Valle - Facultad de Salud ISSN: 0120-8322 EISSN: 1657-9534 Vol. 41, Num. 2, 2010, pp. 155-160

Colombia Médica, Vol. 41, No. 2, April-June, 2010, pp. 155-160

Original Article

Enzyme-linked immunosorbent assay of phenolic glycolipid-I in patients with Hansen’s disease

Inmunoensayo ligado a enzimas (ELISA) a glicolípido fenólico -1 en pacientes con enfermedad de Hansen

Luis Hernando Moreno¹, Alberto Alzate²

¹ Professor and Director of Graduate Studies in Dermatology, Facultad de Salud, Universidad del Valle, Cali, Colombia
² Professor of Epidemiology, Graduate Studies in Health, Universidad Santiago de Cali, Cali, Colombia

Correspondence Address:Luis Hernando Moreno, Professor and Director of Graduate Studies in Dermatology, Facultad de Salud, Universidad del Valle, Cali, Colombia, lhmorenom@hotmail.com

Date of Submission: 11-Sep-2009
Date of Acceptance: 23-Feb-2010

Code Number: rc10021

Abstract

Keywords: ELISA; Hansen; Mycobacterium leprae; Phenolic glycolipid; Leprosy

RESUMEN

Introducción: La enfermedad de Hansen o lepra es una entidad infecto-contagiosa producida por el bacilo de Hansen o Mycobacterium leprae. El glicolípido fenólico I es un trisacárido especial que se encuentra en la pared celular del bacilo y ha demostrado ser específico de especie e inmunogénico durante la infección de M. leprae.
Objetivo: Determinar la presencia del bacilo de Hansen por el método de inmunoensayo ligado a enzimas (ELISA) a glicolípido fenólico I en un grupo de pacientes del Consultorio Dermatológico del Servicio de Salud del Valle del Cauca clasificados como curados o en vigilancia, según criterios preestablecidos por la Organización Mundial de la Salud (OMS).
Metodología: De la base de datos del Consultorio Dermatológico del Servicio de Salud del Valle del Cauca se estudiaron en total 159 pacientes con enfermedad de Hansen a los cuales se les practicó examen de inmunoensayo ligado con enzimas (ELISA) con el glicolípido fenólico I a fin de cruzar información y observar si eran o no positivos a esta prueba. El ELISA positivo dice que el bacilo aún existe en el paciente.
Resultados: Se encontró como dato importante que de 78 pacientes curados, al tener en cuenta además el período de vigilancia, 9 fueron positivos para el ELISA. Cuando se descartó este período, a 81 enfermos se les clasificó como curados según criterios de la OMS pero siguieron positivos para ELISA los mismos 9.
Conclusión: Se concluye que a pesar de cumplir los criterios de la OMS, estos pacientes aún muestran presencia de bacilo y que el período de vigilancia no se necesita como criterio para dar de alta a un paciente. Se recomienda agregar a los criterios de la OMS el ELISA negativo, con el fin de tener una información más que ayude a certificar que un paciente está curado o no.

Palabras clave: ELISA; Hansen; Mycobacterium leprae; Glicolipido fenólico; Lepra.

Hansen′s disease or leprosy is a contagious infection pathology produced by an alcohol-acid resistant bacillus, known as Hansen′s bacillus or Mycobacterium leprae. M. leprae presents a triple coating rich in external polysaccharides and a basal peptidoglycan that forms the wall surrounding the cytoplasm. Peptidoglycans, arabinogalactans, and mycolates significantly contribute to the wall′s structural integrity, but do not contribute major immunogenicity to the bacteria. Other glycolipids, glycopeptidolipids, and trehalose containing lipooligosaccharides have been found to be active mycobacteria components. The most notable of the glycolipid molecules associated with the M. leprae cell wall is the phenolic glycolipid I (pgl-I), a special trisaccharide that has proven to be species specific and immunogenic during M. leprae infection ^[1],[2] .

Clinically, this entity is characterized by the diversity in its presentation and its dependency on the conditions of the host, above all from the immunologic point of view. It is considered the main route of contamination of the upper respiratory pathway, although we cannot discard the role played in some instances by the sick skin. Currently, leprosy is considered an entity whose principal reservoir is the human being. The immunological conditions of the host are essential in determining any of the known clinical forms: undetermined, tuberculoid, dymorphous or borderline, and lepromatous. The undetermined form is a clinically and immunologically unstable condition of the disease. The tuberculoid form is a stable aspect of the disease; the smear is negative; the lepromin is positive. Lepromatous leprosy is another stable form of the disease, lepromin is negative, but there is presence of bacilli in the smears. It is a contagious form of the disease. The intermediate or borderline form is unstable and ends up in one in any of the tuberculoid or lepromatous stable forms. Mycobacteria are present, although not in the proportion as in the lepromatous form; the Mitsuda reaction is generally slightly positive. It is another contagious form of the disease ^[3] .

Analysis of the circulating immune complex in patients, above all those with phenolic glycolipid I as a specific antigen and the role of macrophages has gained importance in diverse research works and has led to better understanding this disease ^{[3],[4],[5],[6],[7],[8],[9],[10]} . Furthermore, a correlation has been noted between the bacillary index and the presence of the phenolic glycolipid I antigen in serum of patients and it has been shown that levels of antibodies towards this antigen diminish when treatment starts ^[11] .

For the diagnosis of the disease and follow up of patients, different laboratory methods are employed like the smear, the biopsy, and the lepromin. A clinical exam and a good clinical history are vitally important. However, it is difficult to precisely define if a patient is or is not cured, and if the bacillus persists in the patient′s organism with the previous laboratory methods.

The enzyme-linked immune-absorbent assay (ELISA) is a method that has acquired scientific credibility and it is currently used to diagnose diverse diseases like tuberculosis, dengue, rubella, and HIV, among others. Brennan and Hunter ^[9] managed to characterize phenolic glycolipid I as a specific M. leprae antigen. Seroepidemiologic studies in different countries to diagnose the infection, have permitted evaluating the ELISA test with a special antigen, phenolic glycolipid I; works have been done with serum from both paucibacillary and multibacillary patients, in whom sensitivity has been between 82 and 100% for the multibacillary and between 5 and 54.5% for the paucibacillary with a global sensitivity between 43.8% and 62.8%. Specificity in endemic and non-endemic areas varied between 82% and 100% ^[3] .

With ELISA-type tests at antibody level in different populations, it has been shown that:

• The serologic response to this antigen from individuals infected is of IgM type ^[12],[13].
• The level of antibodies corresponds to the bacilli load and increases, then, from the tuberculoid pole to the lepromatous ^[12],[14].
• Almost all patients with the lepromatous form have antibodies for this antigen ^[14] .
• Antibody levels diminish with chemeotherapy ^[15] .
• Seropositivity seems to precede the clinical manifestations of lepromatous patients ^[15] .
• In studies with household contacts of leprosy patients, it has been found that 30% offer a positive response to these types of tests, but most do not develop the disease ^{[16],[17],[18]} .

Through the current study, we sought to find the correlation between the behavior of Hansen′s disease in its different clinical states and its evolution with respect to the ELISA method. We also compared this method to those used traditionally to monitor patients such as the smear and, above all, the clinical evaluation.

Methods

Herein, we studied 159 patients with confirmed diagnosis of Hansen′s disease. Respective and previously defined samples were taken from the patients. Included in the study were patients who were registered as sick within the last three years, in addition to those who were in the surveillance period and those who has been discharged as cured. Three groups were formed; the first group gathered those patients who were under control within the last three years; the second group included patients in the surveillance period, and the third group took in those patients who had left the program as cured. The study also included 20 patients with non-lepromatous tuberculosis for the purpose of evaluating the behavior of the Hansen ELISA technique against the Koch bacillus as a microorganism different from M. leprae and that could eventually have a crossed reaction in this test.

The project was approved by the Ethics Committee of the Faculty of Health at Universidad del Valle, Cali, Colombia, and each patient signed an informed consent prior to taking the blood sample. For the ELISA tests, we used the UMELISA HANSEN equipment that detects M. leprae IgM antibodies via fluorescence.

The data were recorded in an Excel data base and they were analyzed by using the Epi Info program version 6.0 to find the average, mode, and median for each variable according to whether it is categorical or continuous, estimate its standard error and deviation for averages or proportions and, thus, calculate the reliability intervals from each of the variables.

The risk factors to become ELISA positive were analyzed on a four-cell table, where the dependent variable is to continue positive for the phenolic glycolipid I antigen after being considered cured according criteria currently employed like: time of treatment for each clinical form, negative smear, remission of symptoms and medical signs, and regularity of treatment; also analyzed was the surveillance time used.

The currently accepted cure criteria are:

• Disappearance of clinical lesions.
• Negative smear.
• Adequate treatment scheme according to clinical form.
• Regularity during treatment.

Results

We studied 159 patients with Hansen′s disease (leprosy); 81 were under treatment and 78 were cured according to clinical cure criteria, negative smear, and adequate pharmacological treatment scheme for each form of the disease. The surveillance period previously used was also considered as additional criteria to discharge patients and evaluate if it is recommended to continue with the surveillance. Of the total number of patients, 130 corresponded to the multibacillary form (66 cured and 64 under treatment) and 29 to the paucibacillary form (11 cured and 18 under treatment). The 20 patients with tuberculosis were ELISA negative, as expected [Table - 1].

Of the 159 patients, 12 corresponded to dymorphous leprosy (DL), 9 to undetermined (UL), 118 to lepromatous (LL), and 20 to tuberculoid (TL); that is, 130 multibacillary (MB) and 29 paucibacillary (PB). Patient ages ranged from 5 to 93 years of age [Table - 2].

Of the 26 patients with elevated ELISA, 25 are LL and 1 LD. With suspect ELISA of the total of 27, 23 are LL, 1 LD, 2 LT, and 1 LI. Of the 106 with normal ELISA, 70 are LL, 10 LD, 18 LT, and 8 LI. The differences do not manage to be statistically significant (p=0.05814026) because of the cells with very small values or equal to zero.

Of the 26 patients with elevated ELISA, 18 show negative smear and 8 are positive. With suspect ELISA in 27 patients, 2 are positive and 25 negative. With a normal ELISA test in 106 patients, 3 are positive for the smear and 103 are negative. The statistical value is p=0.00001724, significant statistical difference. It was expected for the negative smears to also be negative ELISA under 0.3 according to standard figures given by previous studies [Table - 3].

Of the 78 patients considered cured, 9 had elevated ELISA and 14 had suspect ELISA. Only 55 of the 78 were negative. Of the patients with regular treatment, 28 had elevated or suspect ELISA and 47 had already become negative through treatment.

Discussion

Hansen′s disease (leprosy) continues to be a public health problem in the world. Throughout the history of humanity, many efforts have been made to better understand this entity and reach its elimination and subsequent eradication. This disease bears a historic load that makes it feared by everyone. All scientific research undertaken to understand it and treat it in the best possible way are valid and should be considered. This entity reveals immunological behavior that is special and fascinating for any researcher. Since 1943, when diaminodiphenylsulfone (DDS) therapy was introduced, and passing through the establishment of multidrug therapy, the world′s medical and scientific community has dreamt of eradicating this condition. Criteria have been unified to define if a patient is cured or not of the disease and what′s more, in many instances it is difficult conduct an asserted diagnosis. It is common to find relapses in individuals thought to be cured. For this purpose, surveillance was instituted - initially for a lifetime. Then, this surveillance time was reduced to two years for paucibacillary patients and five years for multibacillary patients; all this in agreement with diverse clinical-epidemiological and bacteriological studies that have been carried out. Currently, the conduct recommended by the World Health Organization (WHO) and the Colombian Ministry of Health is to consider as cured every patient WHO:

• Receives adequate stipulated time of treatment for diverse clinical forms.
• Is clinically cured.
• Has a negative smear; i.e., the patient is no longer under surveillance.

In the current study, we found no statistical difference in age or gender of patients and the greatest number of these corresponded to multibacillary forms, i.e., those considered contagious, which endorses every action and/or recommendation for proper treatment of this entity. We consider it important to suggest the execution of the compared surveillance period, again as a cure criterion, given that it is another motive that when added to the ELISA results would furnish tools to aid in deciding if a patient is free from the disease or does not support the disease.

The most important aspect was to find patients who considered themselves cured with high levels of ELISA, suggesting that these were cases of sub-clinical patients that should be watched closely or, ideally, receive treatment until the test proves negative. ELISA is proposed as an additional criterion, which if added to those currently used would yield greater certainty on the cure of the patient with Hansen′s disease and would bring us closer to whether or not the patient is free from the bacillus. Greater numbers of cure criteria available will improve the patient′s welfare and that of the community in general. Furthermore, we propose that the values to define whether a title is high, suspect, doubtful, or negative should be adjusted and we suggest that only values lower than 0.40 be used for negative titles and those greater than 0.40 for positive titles, which would further simplify the analysis that added to pre-existing criteria lead to precise conclusions and actions according to the values obtained.

Furthermore, we suggest that individuals in contact and those living with the patients -especially multibacillary patients- undergo an ELISA test as part of its surveillance to discover possible early or sub-clinical cases of the disease and follow proper conduct. It is important to highlight that a patient can remain positive for the disease through multiple causes like irregularity in its treatment, inadequate time of treatment, and lack of clear criteria to establish if the patient is indeed cured and can be discharged. Additionally, it is obligatory to manifest the importance of the population follow up (contacts and cohabitants) and the use of lepromin, which along with the ELISA test would help in deciding if an individual should or should not receive treatment or should be merely observed with clinical and/or laboratory follow up.

This research presents the following list of conclusions:

• There is no precise method that tells exactly when a leprosy patient is cured from the disease.
• To discharge a leprosy patient, there must be abundant clinical motives and laboratory aids.
• ELISA is a laboratory test that helps to determine if a patient is free or not from the Hansen bacillus.
• It is necessary to conduct surveillance again in the follow up of patients with Hansen′s disease who complete the treatment cycles.
• According to the results from the current research, ELISA is recommended as a laboratory test for patients with leprosy who are going to be discharged and who meet currently accepted requirements: clinical cure, adequate treatment, compliance with time of treatment, surveillance, and negative smear.

References

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