About Bioline  All Journals  Testimonials  Membership  News

African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 3, Num. 1, 2006, pp. 36-43

African Journal. Traditional, Complementary and Alternative Medicines Vol. 3, Num. 1, 2006, pp.36-43   


Claudia E. N. MOMO a, *Julius. E. OBEN a,    Dagobert TAZOOb,   Etienne DONGOb.

a Department of biochemistry, Faculty of Science, university of Yaounde I, P.O.Box 812 Yaounde, Cameroon, b Department of organic chemistry, Faculty of Science, university of Yaounde I, P.O.Box 812 Yaounde, Cameroon E-mail:

 Code Number: tc06004


Laportea ovalifolia (Scham and Thonn) is widly use in Cameroon for the treatment of diabetes mellitus. The present study was designed to evaluate the antidiabetic and hypolipidaemic effects of aqueous extract of Laportea ovalifolia aerial part in normal and alloxan diabetic rats. Diabetes was induced by intraperitoneal injection of alloxan (150 mg kg –1 body weight). The treatment was given for 2 weeks. After the treatment a significant reduction was observed in fasting serum glucose levels in the treated diabetics rats. L. ovalifolia treatment showed considerable lowering of serum total cholesterol, triglycerides, LDL cholesterol, T.C/HDL.C and an increase in HDL cholesterol in the treated diabetic group. These results suggest that the Laportea ovalifolia aqueous extract of the aerial part possesses antidiabetic and hypolipideamic effects in alloxan-induced diabetic rats.

Keywords: Laportea ovalifolia, Alloxan, antidiabetic ,  hypolipidemic


Diabetes is a disorder of carbohydrate, fat and protein attributed to a diminished production of insulin or mounting resistance to its action. Chronic hyperglycaemia during diabetes causes glycation of body proteins that in turn leads to secondary complications affecting eyes, kidneys, nerves and arteries (Kameswara, 1999). Along with hyperglycaemia and abnormalities in serum lipids (Virella and Virella, 2003; NCEP, 2002) diabetes is associated with microvascular and macravascular complications which are the majors causes of morbidity and death in diabetic subjects (Nagappa et al., 2003). It can be managed by exercise, diet and pharmaceutical drugs, which are either too expensive or have undesirable sides effects or contraindications (Serrano, 1990). The search for more effective and safer hypoglycemic agents therefore has continued to be an area of research of interest (Krishna et al., 2004; Pepato et al., 2003). The World Health Organisation has recommended and encouraged the use of alternative therapy especially in countries where access to the conventional treatment of diabetes is not adequate (WHO, 1980).

Laportea ovalifolia (L. ovalifolia) belongs to the family of Urticaceae.  It is a tropical plant commonly found in swampy areas in Cameroon and other parts of the world in both dry and rainy seasons (Letouzey, 1968). Some people in Cameroon use the leaves as vegetable, which serves as a major component of their diet. The leaves of Laportea ovalifolia are used in traditional medicine for the remedy of bacterial infections, headaches, urinary infections, pneumonia, dysentery and epilepsy (Adjanohoun et al., 1996; Letouzey, 1968). Locally, the decoction of the aerial part is used as a cure for diabetes (personal information from users), and this has not previously been reported.

The present communication is to show the effect of aqueous extract of Laportea ovalifolia  on alloxan-induced diabetic rats.

Materials and Methods 

Collection of plant materials 

The aerial part (leaves and stem) of Laportea ovalifolia were collected in October and November 2002, in Yaounde, Cameroon and identified at the National Herbarium, YaoundeCameroon, where a voucher sample had been deposited under the number 50623/HNC.                                  

Preparation of extract 

The plant material was air-dried for 30 days at room temperature and ground into a powder. The plant powder (500g) was decocted in 4L of distilled water for 15min. This was repeated four times, until the resulting extract gave no further colouration.  The extract was then filtered and evaporated to dryness in an oven at 40°C, to obtain 84g of crude residue  (yield: 16.8%).                                                                             

Experimental induction of diabetes in rats 

Three month old male Wistar Albino rats weighing 180-240g were obtained from the animal house of the laboratory of Biochemistry, Department of Biochemistry, University of Yaounde I, Cameroon. All animals were kept in an environmentally controlled room with a 12h light/12h dark cycle .The animals had free access to water and standard rat diet. The study was approved by institutional animal ethical committee. The rats were injected alloxan monohydrate dissolved in sterile normal saline at a dose of 150 mg/kg body weight, intraperitoneally. Since alloxan is capable of producing fatal hypoglycaemia as a result of massive pancreatic insulin release, rats were treated with 20 % glucose solution intraperitoneally after 6h. The rats were then kept for the next 24h on 5 % glucose solution bottles in their cages to prevent hypoglycaemia (Dhandapani et al., 2002). After a fortnight, rats with marked hyperglycaemia were selected and used for the  study.  

Experimental design

In the experiment a total of 25 rats (15 diabetic surviving rats, 10 normal rats) were used. Diabetes was induced in rats 2 weeks before starting the treatment. The rats were  divided into five groups as follows after the induction of alloxan diabetes and each group comprised of 5 rats. Group I (untreated normal rats), Group II (treated normals rats given 200 mg/kg (dose which produces high hypoglycaemic activity in acute treatment after 5h) in distilled water daily using an intragastric gavage tube for 2 weeks), Group III (untreated diabetic rats), Group IV (treated diabetic given aqueous extract of L.ovalifolia  at dose of 200 mg/kg daily using an intragastric gavage), Group V (Diabetic rats given Tolbutamide orally at 80mg/kg in distilled water daily for 2 weeks).

The animals were carefully monitored every day and weighed every week (2 weeks). No sign of toxicity was noticed on the behaviour and general health of the animals when exposed to extract. Animals described as fasted were deprived of food for at least 12 h but allowed free access to drinking water. Blood samples were drawn at weekly intervals till the end of study. Fasting blood glucose estimation, body weight, food and water intake measurement were done on day 0, 7 and 14 of the study.

On day 14, rats were sacrificed by cervical dislocation under ether anaesthesia. Blood was collected from overnight fasted rats and processed for the estimation of serum glucose and serum lipids profile.                      

Determination of Serum glucose

Fasting serum glucose was estimated by the oxidase method  (Trinder, 1969).

Determination of serum total cholesterol, triglycerides, HDL cholesterol, LDL cholesterol and total cholesterol/HDL cholesterol (T.C/HDL C.). Serum was separated and analysed for serum total cholesterol (Roeschlau et al.,1974), triglycerides (Buccolo et al., David, 1973), HDL cholesterol (Allain et al., 1974) and serum LDL ( Friedewald et al., 1972).                                                                                  

Statistical analysis.  

Data was expressed as means ± S.E.M.  Statistical analysis was made by  one-way ANOVA and post hoc Dunnet test, with P < 0.05 considered as significantly different.                                                                                                                                                    


Changes in body weight in untreated and treated rats are shown in Table 1. Significant (P < 0.001) weight loss was observed in untreated diabetic rats than untreated normal rats. Treatment with aqueous extract of L. ovalifolia or Tolbutamide improved the weight gain compared to untreated diabetic rats. Alteration in blood glucose on treatment of diabetic rats with Laportea ovalifolia and tolbutamide is given in Table 2.  The blood glucose was increased significantly in untreated alloxan-diabetic rats as compared to untreated normal rats (P < 0.001). Administration of L. ovalifolia or Tolbutamide led to significant decrease in blood glucose levels in diabetics treated groups (P < 0.001), while no decrease in blood glucose levels was observed in normal treated group.

The food and water intake (Tables 3 and 4) (P < 0.05) increased significantly in untreated diabetic rats  compared to untreated normal rats. Significant reduction (P < 0.05) of food intake and water intake were noticed after the treatment with of L. ovalifolia and Tolbutamide. No effect of the extract was noticed in normal rats. Serum total cholesterol, triglycerides, LDL cholesterol and (T. C/HDL. C) were significantly elevated in untreated diabetic rats as compared to untreated normal rats (P < 0.001). All lipids parameters tested were improved after the treatment with aqueous extract of L. ovalifolia and Tolbutamide. No effect was observed in treated normal rats. (Table 5)


Alloxan induces diabetes by damaging the insulin secreting cells of the pancreas leading to hyperglycaemia (Szuldelski, 2001). An observation in this study correlates with the previous research finding, in that the blood glucose levels significantly increased in alloxan untreated diabetic rats. In the present study, the continuous treatment with aqueous extract of L. ovalifolia for a period of 2 weeks caused a significant decrease in the blood glucose levels of treated diabetic rats but no effect was observed in normal treated rats. These results have confirmed the earlier results of our preliminary studies (not published). The possible mechanism by which aqueous extract of L. ovalifolia brings about its hypoglycaemic action may be, by potentiating the insulin effect, either by increasing the pancreatic secretion of insulin from the cells of islets of Langerhan’s or its release from bound insulin (Pari, 2004). We have noticed a significant reduction in food and water intake and increased in the body weight in alloxan diabetic rats. This could be the result of improved glycaemic control produced by aqueous extract of L. ovalifolia.

We have noticed elevated serum lipids in alloxan-diabetic rats. Lipids play an important role in the pathogenesis of diabetes mellitus. The level of serum lipids is usually raised in diabetes and such an elevation represents a risk factor for coronary heart disease (Mironava et al., 2000). Lowering of serum lipids levels through dietary or drugs therapy seems to be associated with a decrease in the risk of vascular disease (Scoot and Grundy, 1999). The abnormal high concentration of serum lipids in diabetes is mainly due to the increase in the mobilisation of fatty acids from the peripheral depots, since insulin inhibits the hormone sensitive lipase. On the other hand, glucagon, catecholamines and other hormones enhance lipolysis. The marked hyperlipidaemia that characterizes the diabetic state may therefore be regarded as a consequence of the uninhibited actions of lipolytic hormones on the fat depots (AL-Shamaony et al.,1994).

In our study, we have also observed an increase in the concentration of total cholesterol, triglycerides, LDL cholesterol and T.C/HDL.C in alloxan untreated diabetic rats. Hyperlipidaemia is a recognized consequence of diabetes mellitus (Pushparaj et al., 2000; Pepato et al., 2003; Sharma et al., 2003). Administration of aqueous extract of L. ovalifolia normalized serum lipids, secondary to the diabetic state. Diabetes-induced hyperlipidaemia is attributable to excess mobilization of fat from the adipose due to the under utilization of glucose (Krishnakumar et al., 2000). The ability of aqueous extract of L. ovalifolia to reduce the levels of plasma  lipids in diabetic rats has never been studied before. The regression of the diabetic state due to aqueous extract of L. ovalifolia administration increased the utilization of glucose, thereby depressing the mobilization of fat.

Our findings indicate that an aqueous extract of L. ovalifolia can lower the blood glucose and serum lipids in alloxan diabetics rats. This is of interest, since elevated concentrations of both are risk factors in the development of arteriosclerosis in diabetes mellitus.

  1. Adjanohoun, J. E., Aboubakar, N., Dramane, K., Ebot, M. E., Ekpere, J. A., Enoworaa E.G., Focho, D., Ogbile, Z., Kamanyi, A., Kamsu-Kom, J., Keita, A., Mbenkum, T., Mbi, C. N., Mbiele, A. L., Mbome, I. L., Muburu, N. K., Nancy, W. L., Nkongmeneck, B., Satabie, B., Sofowora, A., Tamzé, V. and Virmum, C. K. (1996).Traditional medicine and pharmacopoeia, contribution of ethnobotanical and floristic studies in Cameroon. Centre National de Production de Manuels Scolaires.  Porto-Novo,Benin, pp. 423-464.
  2. Allain, C.  C., Poon, L. S., Chan, C. S., Richmond, W. and Fu, P. C. (1974). Enzymatic determination of serum total cholesterol. Clin. Chem. 20: 470-475.
  3. Al-shamaony, L., Al-khaznaji, S.M. and Tway, H.A.A. (1994). Hypoglycaemic effect of Artemisia herba alba II. Effect of valuable extract on some blood parameters, in diabetic animals. J.  ethnopharmacol. 43 : 167-171.
  4. Dhandapani, S., Ramasamy, S.V., Rajagopal, S. and Namasivayam, N. (2002). Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacol. Res. 46 (3): 251-255.
  5. Friedewald, W. T., Levy, R. I. and Frederickson, D. S. (1972). Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin. Chem. 18: 499-502.
  6.   Kameswara  Rao, B., Kesavulu, M. M., Giri, R.  andAppa Rao, ch. (1999). Antidiabetic and hypolipidemic effects of momordica cymbalaria Hook, fruit powder in alloxan diabetic rats. J.  of ethnopharmacol. 67: 103-109.
  7. Krishnakumar, K., augustti, K. T.and Vijayammal, P. L. (2000). Hypolipidaemic effect of Salacia oblonga Wall. root bark in streptozotocin diabetic rats. Med. Science. 28: 65-67.
  8. Krishna, B., Nammi, S., Kota, M. K. and Krishna Rao, R. V. (2004). Evaluation of hypoglycaemic and antihyperglycemic effects of Datura metel Linn seeds in normal and alloxan-induced diabetic rats.J.  of ethnopharmacol. 9: 95-98.
  9. Letouzey, R. (1968). Flore du Cameroun. Muséum National d’Histoire Naturelle. Paris, 8: pp 67-136.
  10. Mironova, M. A., Klein, R. L., Virella, G. T. and Lopes-Virella, M. F. (2000). Anti-modified LDL antibodies, LDL-containing immunune complexes, and susceptibility of LDL to in vitro oxidation in patients with type 2 diabetes. Diabetes. 49:1033-1049.
  11. Nagappa, A. N., Thakurdesai, P. A., Venkat Rao, N. and Jiwan Singh. (2003). Antidiabetic activity of Terminalia catappa Linn fruits. J. of ethnopharmacol. 88: 45-50.
  12. NCEP (Third Report of the National Cholesterol Education Program). (2002). Expert Panel  on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adult (Adult Treatment Panel III) final report. Circulation. 106 : 3143-3421.
  13. Pari, L and Amarnath Satheesh. (2004). Antidiabetic activity of Boerhaavia diffusa L.: effect on hepatic key enzymes in experimental diabetes.J. of ethnopharmacol. 91: 109-113.
  14. Pepato, M. T., Baviera, A. M., Vendramini, R. C., Perez, M. P., Kettelhut, I. C. and Brunetti, I. L. (2003). Cissus sicyoides (Princess wine) in the long terme treatment of streptozotocin – diabetic rats. Biotchnol. Appl. Biochem. 37: 15: 20.
  15. Pushparaj. P., Tan, C. H. and Tan, B. K. H. (2000). Effects of Averrhoa  bilimbi leaf extract  on blood glucose and lipids in streptozotocin-diabetic ratsJ.  of ethnopharmacol. 72 : 69-76.
  16. Roeschlau, P., Bertnt, E. and Gruber, W. A.(1974). Enzymatic determination of total cholesterol  in serum. Clin. Chem. Clin. Bioch. 12: 226.
  17. Scott. M and Grundy. (1999). Diabetes and cardiovascular disease. Circulation. 100 : 1134-1146.
  18. Serrano, J. J. (1990). Toxico-pharmacologie expérimentale des plantes médicinales. Actes du 1er Colloque Europeen d’Ethnopharmacologie. Office de la Recherche Scientifique et Techniques d’Outre Mer (ORSTOM). pp210-218.
  19. Sharma, S. B., Hasir., A., Prabhu, K. M., Murthy, P. S. and Dev, G. (2003). Hypoglycaemic and hypolipidemic effect of ethanolic extract of seeds of Eugenia jambolona in alloxan-induced diabetic rabbits.  J.  of  ethnopharmacol. 85: 201-206.
  20. Szudelski, T. (2001). The mechanism of alloxan and streptozotocin action in ß  cells of the rat pancreas.  Physiolo. Res. 50: 536-546.
  21. Trinder, P. (1969). Determination of blood glucose using an oxydase-peroxidase system with a non-carcinogenic chromogem. J. of Clin. pathol. 21: 158-161.
  22. Virella-Lopes, M. F. and Virella, G. (2003). The role of immune and inflammatory processes in the development of macrovascular disease in diabetes. Frontiers in Biosc. 8: 750–768.
  23. WHO Expert Committee on Diabetes mellitus, 1980. Second Report, Technical Report series 646. World Health Organisation, Geneva.

© Copyright 2006 -African. Journal. Traditional, Complementary and Alternative Medicines

The following images related to this document are available:

Photo images

[tc06004t5.jpg] [tc06004t4.jpg] [tc06004t2.jpg] [tc06004t1.jpg] [tc06004t3.jpg]
Home Faq Resources Email Bioline
© Bioline International, 1989 - 2024, Site last up-dated on 01-Sep-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Google Cloud Platform, GCP, Brazil