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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 4, Num. 2, 2007, pp. 219-225

African Journal of Traditional, Complimentary and Alternative Medicines, Vol.4, No. 2, 2007, pg. 219-225

Research Paper

BRINE SHRIMP TOXICITY EVALUATION OF SOME TANZANIAN PLANTS USED TRADITIONALLY FOR THE TREATMENT OF FUNGAL INFECTIONS

Mainen J. Moshi*a, Carolien J.P. van den Beukelb, Omar J.M. Hamzac, Zakaria H. Mbwamboa, Ramadhani O.S. Nondoa, Pax J.Masimbaa, Mecky I.N. Mateed, Modest C.Kapingua, Frans Mikxf, Paul E. Verweije, and André J.A.M. van der Venb.

aInstitute of Traditional Medicine,Muhimbili University College of Health Sciences (MUCHS), Dar es Salaam, Tanzania.,bDepartment of General Internal Medicine, Radboud University Nijmegen Medical        Center, Nijmegen, The Netherlands,.cDepartment of Oral Surgery and Oral Pathology, MUCHS, Dar es Salaam, Tanzania, .dDepartment of Medical Microbiology and Immunology, MUCHS, Dar es Salaam, Tanzania, .eWHO Collaborating Centre, Dentistry, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.,fDepartment of Medical Microbiology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands.
E-mail: mmoshi@muchs.ac.tz;  Tel: 255 22 2150096; Fax 255 22 2150465.

Code Number: tc07022

Abstract

Plants which are used by traditional healers in Tanzania have been evaluated to obtain preliminary data of their toxicity using the brine shrimps test.  The results indicate that 9 out of 44 plant species whose extracts were tested exhibited high toxicity with LC50 values below 20 μg/ml.  These include Aloe lateritia Engl. (Aloaceae) [19.1μg/ml], Cassia abbreviata Oliv. (Caesalpiniaceae) [12.7μg/ml], Croton scheffleri Pax  (Euphorbiaceae) [13.7μg/ml], Hymenodactyon parvifolium Brig (Rubiaceae) [13.4μg/ml], Kigelia Africana L. (Bignoniaceae) [7.2μg/ml], and Ocimum suave Oliv. (Labiatae) [16.7μg/ml]. Twelve plants gave LC50 values between 21 and 50μg/ml, 11 plants gave LC50 values between 50 and 100 μg/ml, and 18 plants gave LC50 values greater than 100 μg/ml. 

Key words: Brine shrimp test; Toxicity evaluation; Traditional antifungal plantsIntroduction

In sub Saharan Africa, where 70% of the world cases of HIV/AIDS are found, Candida infections are very common and cause significant morbidity among patients (UNAIDS, 2004). Among problems that hamper effective management of Candida infections in these countries include;  limited number of effective antifungal agents, toxicity of the available antifungal agents, resistance of Candida to commonly used antifungals, relapse of candida infections and the high cost of antifungal agents (Debruyne, 1997;Sangeorzan et al., 1994).   Reports of resistance to commonly used antifungal agents like fluconazole abound (Ruhnke et al., 1994; Redding et al., 1994), including shifts from Candida albicans to less sensitive species such as Candida glabrata and Candida krusei (Bastert et al., 2001; Powderly, 1992).  When relapses occur, the infections tend to be increasingly refractory to treatment.

These problems are of even greater relevance to poor countries, where the choice of antifungal agents is rather limited due to limited resources.  In these countries, the most practical option remains to search for cheap alternatives to manage opportunistic infections.  The difficulties associated with the management of Candida infections necessitate the discovery of new antifungal agents, in order to widen the spectrum of activity against Candida and combat strains expressing resistance to the available antifungal agents.

Plants are widely used in Tanzanian traditional medicine and constitute a potentially useful resource for new and safe drugs for the treatment of opportunistic infections. According to Medicine du Monde, a French non-governmental organisation, in Kagera region, five out of every six HIV patients receive their medical attention from a traditional healer rather than from a hospital or primary health care facility (AIDS Analysis Africa, 1996). Likewise, a survey conducted in Dar es Salaam showed that 21% of the people who seek care from public facilities had first consulted a traditional healer (Kilima et al., 1993).

The purpose of the present study was to evaluate the toxicities and/or potential for other biological activities of extracts of the plants that are used by traditional healers in Tanzania for management of fungal infections.

 Materials and Methods        

Plant collection and identification

Plants reported to be used for the treatment of oral candidiasis and skin fungal infections by the interviewed traditional healers (table 1a, b) were collected in four regions of Tanzania from February-March 2004. The plants were identified by Mr. Selemani, an experienced botany technician, and voucher specimens are kept at the Herbarium of the Department of Botany, University of Dar es Salaam.

Extraction of plant materials

All plant samples were air-dried and ground. Approximately 400 grams of the plant materials were macerated with 80% methanol at room temperature and after 24 h filtered through Whatman number 1 filter paper. The procedure was repeated three times to ensure exhaustive extraction of the plant material. The extracts were pooled together, concentrated, and the solvent removed by evaporation under reduced pressure in a rotar vapor, at 40°C. The extracts were further dried by freeze-drying and kept in a freezer, at -20°C, until the time of use.

The Brine shrimp lethality test

The brine shrimp lethality test (BST) was used to predict the presence, in the extracts, of cytotoxic activity (Meyer et al., 1982).  Solutions of the extracts were made in DMSO, at varying concentrations, and 30 µl of each incubated in duplicate vials with the brine shrimp larvae in a total volume of 5 ml. Ten brine shrimp larvae were placed in each of the duplicate vials. Brine shrimp larvae were placed in a mixture of DMSO (30 µl) and seawater to serve as a negative control.  Cyclophosphamide, an anticancer drug, was used as a positive control. After 24 h the nauplii were examined against a lighted background, with a magnifying glass and the average number of survived larvae was determined. The mean percentage mortality was plotted against the logarithm of concentrations and the concentration killing fifty percent of the larvae (LC50) was determined from the graph.

Data analysis

The mean results of brine shrimp mortality against the logarithms of concentrations were plotted using the Fig P computer program (Biosoft Inc, USA), which also gives the regression equations. The regression equations were used to calculate LC16, LC50 and LC84 values. Confidence intervals (95% CI) were calculated according to a previously reported method (Litchfield and Wilcoxon, 1949).

Results

Brine shrimp lethality

Among the 65 plant parts collected and belonging to 56 plant species, 50 (76.9%) plant parts of 44 plant species were tested for brine shrimp lethality.  Nine plants showed high toxicity to the shrimps with LC50 values below 20µg/ml (Table 2).  These include Aloe lateritia (19.1µg/ml), Cassia abbreviata (12.7µg/ml), Croton scheffleri (13.7µg/ml), Hymenodactyon parvifolium (13.4µg/ml), Kigelia Africana (7.2µg/ml), and Ocimum suave (16.7µg/ml). Twelve plants gave LC50 values between 21 and 50µg/ml, 11 plants gave LC50 values between 50 and 100 µg/ml, and 18 plants gave LC50 values greater than 100 µg/ml.

Discussion

Previous investigations of our group on the in vitro antifungal activity of the plants support the therapeutic claims of the traditional healers (Hamza et al., in Press). Identification of herbal medicines for the treatment of fungal infections in HIV/AIDS patients could be pivotal in supporting the needs of these patients in terms of easy availability, affordability, and possibly to cope with the problem of recurrent Candida infections and emergence of resistance.

Apart from efficacy, safety of herbal medicines is of paramount importance as there is not much that is known about many plants that are used in traditional medicine. We have used the brine shrimp lethality test as a preliminary tool to evaluate the toxicity of the identified plants.  Unfortunately not all the plants collected were tested.  However, among those tested 9 were quite toxic to the shrimps.  Since the test is also used to identify potential anticancer substances, the results may mean that these plants are either outright toxic or may have potential anticancer activity. Two of the plants Euphorbia heterophylla L. (Rocha e Silva, 1943) and Jatropha multifida are reported to be toxic (Levin et al., 2000), thus supporting what was reported by the healers. The extracts of the roots and leaves of Jatropha multifida also exhibited relatively high toxicity on the shrimps, while for Euphorbia heterophylla the toxicity was low (LC50 80.2 µg/ml).  Toxicity results from animals will be crucial as a way to definitively judge the safety of these plants, as and when they are found to have enough potential for development.  The present results only suggest possibility of other hitherto unreported biological activities, of toxic nature or even anticancer activity. Among the plants tested were seven plants that in earlier investigations of our group showed to have potent antifungal activity(Hamza et al., in Press). The toxic effect of these plants are shown in Table 2. All these plants need to be further investigated for their potential as a source of antifungal compounds.

The results of this toxicity study showed  the relative toxicities of the plants.  More work is needed in order to determine their usefulness as potential antifungal and anticancer agents.  

Acknowledgements

We are grateful to Mr. Selemani of Botany Department, UDSM, for helping with collection and identification of the plants used in this study.  We are grateful to the NAPRALERT Data base of the University of Illinois, at Chicago, for allowing us access to literature.

References
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