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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 6, Num. 4, 2009, pp. 526 - 528

African Journal of Traditional, Complementary and Alternative Medicines, Vol. 6, No. 4, 2009, pp. 526-528

Short Communication


A. Sowemimo 1 , M. van de Venter 2 *, L. Baatjies 2 , T. Koekemoer 2 .

1Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, College of Medicine Campus, Idi-Araba, Lagos
2Department of Biochemistry and Microbiology, PO Box 77000, Nelson Mandela Metropolitan University, Port Elizabeth 6031, South Africa.

Code Number: tc09042


Cancer is one of the most prominent human diseases which has stimulated scientific and commercial interest in the discovery of new anticancer agents from natural sources. The current study investigates the cytotoxic activity of ethanolic extracts of sixteen Nigerian plants used locally for the treatment of cancer using the MTT assay on the HeLa cell line. Sapium ellipticum leaves showed activity comparable to the reference compound Cisplatin and greater cytotoxic activity than Combretum paniculatum, Celosia trigyna, Drymaria cordata, Cyathula achyranthoides and Cyathula prostata. Justica extensa, Pupalia lappacea, Hedranthera barteri leaves, Alternanthera sessilis, Ethulia conyzoides leaves, Combretum zenkeri root, Sapium ellipticum stembark and Lannea nigritana stembark showed very low activity while Combretum molle, Adenanthera parvoniana and Lannea acida showed no activity. The results justify the use of Sapium, Combretum, Celosia, Drymaria and Cyathula in traditional treatment of cancer.

Keywords: Medicinal plants; Cytotoxicity; Cancer; Sapium; Combretum


Plants have formed the basis for the treatment of diseases in traditional medicine systems for many years, and continue to play a major role in the primary health care of about 80% of the world's inhabitants (Farnsworth et al., 1985; Sofowora, 1984; Koduru et al, 2007a). Research interest has focused on various plants that possess anticancer properties and this has led to the discovery and development of efficacious anticancer agents such as vinblastine and vincristine from Catharanthus roseus, and taxol from Taxus brevifolia. (Noble, 1990; Wani et al, 1971). Although the use of ethnomedicines is widespread in Africa, many of these plants are yet to be investigated for their anticancer activity.

This paper reports the cytotoxic activity of the ethanolic extracts of sixteen plants against HeLa cervix adenocarcinoma cells. Sapium ellipticum, Combretum paniculatum, Celosia trigyna, Pupalia lappacea, Justica extensa, Hedranthera barteri, Alternanthera sessilis, Ethulia conyzoides, Lannea nigritana, L. acida, Combretum zenkeri, C. molle, Adenanthera parvoniana, Cyathula achyranthoides, Drymaria cordata and Cyathula prostata were selected based on their frequency in recipes for the management of cancer from an ethnobotanical survey of traditional medical practitioners in Western Nigeria.

Materials and Methods

Plant material

All the tested plants collected from the Olokemeji Forest Reserve and from the Campus of Obafemi Awolowo University, Ile-Ife in Nigeria in July 2006 were authenticated by comparison with corresponding herbarium specimens at the Forestry Research Institute, Ibadan, Nigeria (FRIN) where voucher specimens were also deposited. The plants were air dried for two days followed by drying in a hot air oven at 40°C, ground to powder and stored in amber coloured bottles. 100 g each of powdered plant material was macerated with 80% ethanol at room temperature respectively. Extracts were filtered and concentrated to dryness in vacuo at room temperature. The respective plant parts used are as stated in Table 1.

Cytotoxicity assay

The cytotoxic effect of plant extracts on HeLa (cervix adenocarcinoma) cell line was determined using a modification (Koduru et al., 2007b) of the MTT assay (Mossman, 1983). Briefly, cells were seeded into 96- well culture plates (Nunc) at 6 000 cells/well in RPMI1640:10% fetal bovine serum (FBS) and left for 24 hours. Plant extracts or cisplatin (positive control) were added and the cells incubated for a further 48 hrs after which the medium was replaced with 200 μl MTT (Sigma) (0.5 mg/ml in RPMI 1640:10% FBS). After a further 4 hr incubation at 37°C, the MTT was removed and the purple formazan product dissolved in DMSO and absorbance measured at 540 nm on a multiwell scanning spectrophotometer (Multiscan MS, Labsystems). All incubation steps were carried out in a 37°C humidified incubator with 5% CO2.

Results and Discussion

The cytotoxicity results are as shown in Figure 1 with extract numbers corresponding to those in Table 1. Cisplatin at 10 and 100 μM caused 49.25 ± 3.33% and 88.19 ± 0.60% (SEM, n=4) inhibition, respectively. In consideration of the cytotoxicity, the extracts could be classified into four categories. Firstly, potentially cytotoxic are Sapium ellipticum leaves, Combretum paniculatum leaves, Celosia trigyna, Drymaria cordata and Cyathula prostata showing over 50% activity at 500 μg/ml. Secondly, moderate cytotoxic are Ethulia conyzoides leaves, Hedranthera barteri leaves and Cyathula achyranthoides showing between 40-50% activity at 500 μg/ml. Thirdly, low cytotoxic activities are Pupalia lappacea, Justica extensa, Sapium ellipticum stembark, Alternanthera sessilis, Lannea nigritana stem-bark and Combretum zenkeri root showing less than 40% activity at 500 μg/ml. Lastly, non-toxic are Combretum molle, Adenanthera parvoniana fruit and Lannea acida stem bark with no activity against the HeLa cell line.

The plants in the first three categories justify their inclusion in traditional recipes for the treatment of cancer. The crude extracts in the first two categories could be taken for further bioassay guided experiments. However, Sapium leaves possessed the highest activity indicating its potential for biopharmaceutical use.


This work has been supported by the African Laser Centre (ALC 2008-2009-02).


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© Copyright 2009 - African. Journal. Traditional, Complementary and Alternative Medicines

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