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Ciência Florestal
Centro de Pesquisas Florestais - CEPEF, Departamento de Ciências Florestais - DCFL, Programa de Pós Graduação em Engenharia Florestal - PPGEF
ISSN: 0103-9954
EISSN: 0103-9954
Vol. 29, No. 1, 2019, pp. 116-129
Bioline Code: cf19010
Full paper language: Portuguese
Document type: Research Article
Document available free of charge

Ciência Florestal, Vol. 29, No. 1, 2019, pp. 116-129

 en Environments for seedling production and nodulation by rhizobia in Tachigali vulgaris
Souza, Juciane Casaes de; Pedrozo, Cсssia Тngela; Silva, Krisle da; Oliveira, Vanњbia Ximendes Aragуo & Alencar, Andressa Maria da Silva

Abstract

This study had as objectives to determine the best shading condition and the type of substrate to produce seedlings and the establishment of a spontaneous symbiosis by rhizobia in taxi-branco (Tachigali vulgaris). A randomized block design with four replications and treatments arranged in a split-plot scheme was installed. The plot consisted of two shading levels (50% and 75%) and subplots of four types of substrates: soil; soil + sand (1:1); soil + sand + sawdust (2:1:1); and soil + vermiculite (1:1). Each experimental plot consisted of five seedlings. The seedlings were evaluated for a set of shoot and root morphological and physiological traits. At the end of the experiment, the isolation and the phenotypic characterization of bacteria from nodules collected in the seedlings were also carried out. The characterization was performed by the morphological evaluation of the colonies. The shading of 50% is indicated for seedling productions, while in relation to the substrate, that one composed by soil + sand is what produces seedlings of better quality. Although the number of isolates was not influenced by the substrate type and the shading level, taxibranco seedlings establish symbiosis with a great diversity of rhizobia. Most of the isolates have very fast growth rate and acid pH of the culture medium.

Keywords
Taxi-branco; Nursery; Biological fixation of nitrogen

 
 pt Ambientes para a produчуo de mudas e nodulaчуo por rizѓbios em Tachigali vulgaris
Souza, Juciane Casaes de; Pedrozo, Cсssia Тngela; Silva, Krisle da; Oliveira, Vanњbia Ximendes Aragуo & Alencar, Andressa Maria da Silva

Resumo

O presente estudo teve como objetivos determinar a melhor condiчуo de sombreamento e o tipo de substrato para a produчуo de mudas e estabelecimento da simbiose espontтnea por rizѓbios em taxi-branco (Tachigali vulgaris). O experimento foi instalado no delineamento de blocos casualizados, com quatro repetiчѕes e os tratamentos arranjados em esquema de parcela subdividida, no qual a parcela consistiu de dois nэveis de sombreamento (50% e 75%) e a subparcela de quatro tipos de substrato: solo; solo + areia (1:1); solo + areia + serragem (2:1:1); e solo + vermiculita (1:1). Cada parcela experimental foi constituэda por cinco mudas. As mudas foram avaliadas quanto a um conjunto de variсveis morfolѓgicas e fisiolѓgicas, da parte aщrea e da raiz. No final do experimento foi tambщm realizado o isolamento e a caracterizaчуo fenotэpica de bactщrias oriundas de nѓdulos coletados nas mudas. A caracterizaчуo foi realizada pela avaliaчуo morfolѓgica das colєnias. O sombreamento de 50% щ o indicado na produчуo de mudas de taxi-branco, enquanto que, em relaчуo ao substrato, aquele composto por solo + areia щ o que proporciona mudas de melhor qualidade. A espщcie apresentou simbiose com grande diversidade de rizѓbios, apesar de o nњmero de isolados nуo ter sido influenciado pelo tipo de substrato e nэvel de sombreamento. A maioria dos isolados obtidos apresentam tempo de crescimento muito rсpido e pH сcido do meio de cultura.

Palavras-chave
Taxi-Branco; Viveiro; Fixaчуo biolѓgica de nitrogъnio

 
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Alternative site location: http://cascavel.ufsm.br/revistas/ojs-2.2.2/index.php/cienciaflorestal/index

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